FastQCFastQC Report
Thu 2 Feb 2017
SRR4063915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328876
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40770.1750629917608323No Hit
TATCAACGCAGAGTACTTTTTTTTT24600.10563035558784581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAA456.7621475E-414.77834514
GGTATCA9450.014.6749741
CGCGCTA1207.403287E-1012.66797116
TCTATAC2200.012.0872183
GTATCAA23250.011.9701881
GCGTTAT1001.9343497E-611.3982331
GAACCGT600.00587828611.0830456
ACGGTAT1752.0008883E-1110.858269
CTTACAC3000.010.7633813
TAGACTG2750.010.7081225
TGCACCG909.5275616E-510.5546015
CGTCGTA2001.8189894E-1210.45062510
AAGACGG2950.010.30415255
GAGCGAA2600.010.23182216
GTCCTAT3350.010.2073741
GTCTTAA2053.6379788E-1210.19354251
ACGCGCG1502.4439942E-810.13394213
GTTTAAG2450.010.0800711
TGCACTA856.60106E-410.0579135
ATTACAC1701.5679689E-910.0557533
Produced by FastQC (version 0.11.5)