FastQCFastQC Report
Thu 2 Feb 2017
SRR4063915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328876
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26550.028.0891061
GGTATCA10800.024.6470181
TCAACGC37600.019.4823134
ATCAACG37750.019.3466263
CAACGCA38500.019.0268825
TATCAAC39350.018.951712
AACGCAG39900.018.4144066
TACCGTC2600.017.7676537
CGTTAAG750.001291940917.5988142
ATCGATT1307.320905E-716.92375429
GTATAGG2800.016.4992431
ACGCAGA45950.016.0377487
AGAGTAC47000.015.72761311
CCTATAC2252.5465852E-1115.6430553
CGCAGAG47050.015.52253158
CAGAGTA48550.015.2708110
CGTCGTA2601.8189894E-1215.23072310
TAAGACT4300.014.8358934
CGGATCG1351.956377E-514.66756826
ATACCGT2850.014.6653656
Produced by FastQC (version 0.11.5)