##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2328876
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.365371535453153	32.0	32.0	32.0	32.0	32.0
2	31.462946502948203	32.0	32.0	32.0	32.0	32.0
3	31.51629326765358	32.0	32.0	32.0	32.0	32.0
4	31.590369774947227	32.0	32.0	32.0	32.0	32.0
5	31.558408004548117	32.0	32.0	32.0	32.0	32.0
6	35.154792698280204	36.0	36.0	36.0	36.0	36.0
7	35.16968829598484	36.0	36.0	36.0	36.0	36.0
8	35.119444315626936	36.0	36.0	36.0	36.0	36.0
9	35.208814037329596	36.0	36.0	36.0	36.0	36.0
10	35.0854291941692	36.0	36.0	36.0	36.0	36.0
11	35.207612599382706	36.0	36.0	36.0	36.0	36.0
12	35.126751703396835	36.0	36.0	36.0	36.0	36.0
13	35.16313191427968	36.0	36.0	36.0	36.0	36.0
14	35.111899044861126	36.0	36.0	36.0	36.0	36.0
15	35.090009944711525	36.0	36.0	36.0	36.0	36.0
16	35.099475025720565	36.0	36.0	36.0	36.0	36.0
17	35.07265693836855	36.0	36.0	36.0	36.0	36.0
18	35.063498013634046	36.0	36.0	36.0	36.0	36.0
19	35.066832669493785	36.0	36.0	36.0	36.0	36.0
20	35.057129705488826	36.0	36.0	36.0	36.0	36.0
21	35.05293927199215	36.0	36.0	36.0	36.0	36.0
22	35.03811795904977	36.0	36.0	36.0	36.0	36.0
23	34.98999388546234	36.0	36.0	36.0	36.0	36.0
24	34.96838474869422	36.0	36.0	36.0	36.0	36.0
25	34.94340746351459	36.0	36.0	36.0	36.0	36.0
26	34.883961619253235	36.0	36.0	36.0	32.0	36.0
27	34.88149819913126	36.0	36.0	36.0	32.0	36.0
28	34.86079851396124	36.0	36.0	36.0	32.0	36.0
29	34.84444083755425	36.0	36.0	36.0	32.0	36.0
30	34.831000877676615	36.0	36.0	36.0	32.0	36.0
31	34.81913893225745	36.0	36.0	36.0	32.0	36.0
32	34.788400498781385	36.0	36.0	36.0	32.0	36.0
33	34.76695453085523	36.0	36.0	36.0	32.0	36.0
34	34.76338456834971	36.0	36.0	36.0	32.0	36.0
35	34.7342129851482	36.0	36.0	36.0	32.0	36.0
36	34.71319426195298	36.0	36.0	36.0	32.0	36.0
37	34.704180901001166	36.0	36.0	36.0	32.0	36.0
38	34.680724950577016	36.0	36.0	36.0	32.0	36.0
39	34.67453827511641	36.0	36.0	36.0	32.0	36.0
40	34.65985050298943	36.0	36.0	36.0	32.0	36.0
41	34.65226701636326	36.0	36.0	36.0	32.0	36.0
42	34.60296254502172	36.0	36.0	36.0	32.0	36.0
43	34.58666970675983	36.0	36.0	36.0	32.0	36.0
44	34.54721762773114	36.0	36.0	36.0	32.0	36.0
45	34.5187592641257	36.0	36.0	36.0	32.0	36.0
46	34.50696258624332	36.0	36.0	36.0	32.0	36.0
47	34.48944469349163	36.0	36.0	36.0	32.0	36.0
48	34.44531224504868	36.0	36.0	36.0	32.0	36.0
49	34.43048234427252	36.0	36.0	36.0	32.0	36.0
50	33.8575570360981	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	24.0
21	59.0
22	198.0
23	616.0
24	1663.0
25	3795.0
26	7580.0
27	14337.0
28	23941.0
29	37597.0
30	53835.0
31	76564.0
32	110428.0
33	175851.0
34	371211.0
35	1451169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.483350553492855	17.58796667276458	11.809722819846211	27.11895995389635
2	15.959312386371735	19.93940398460023	37.050693341738	27.05059028729004
3	18.336719814089935	23.260614169630912	28.804938708419712	29.59772730785944
4	12.69273245977888	15.96426774117643	35.30458470094586	36.038415098098824
5	14.444006464921275	36.42886096125341	33.725840276596955	15.401292297228364
6	34.982216689732226	35.18690832331058	16.42398091090568	13.40689407605151
7	30.61495357200365	30.37320594707745	20.381729375771563	18.630111105147336
8	28.08367641729315	33.6635355424677	18.90134983571474	19.351438204524417
9	27.41265209236498	14.223556024266074	18.05751169677025	40.3062801865987
10	15.650846895774666	27.206295961368188	32.1118333535149	25.031023789342242
11	37.20721927659523	21.106104404012925	22.260266325901423	19.426409993490424
12	24.53679331728552	23.463484928768153	28.984779769107305	23.014941984839023
13	29.890710664083432	19.688998584717698	24.762416354067906	25.657874397130964
14	23.60864985651846	19.11144928164763	25.20199701915649	32.07790384267742
15	25.21841852364279	27.190089600332534	21.963117263673006	25.628374612351674
16	25.64238428795511	25.852357996155206	23.56351575854827	24.941741957341414
17	24.34037893832859	25.849047286780095	25.075154312704633	24.735419462186677
18	24.807900150588246	25.453535989818228	25.64581833140321	24.092745528190317
19	25.577360065542347	24.984799534195894	25.251709408315428	24.18613099194633
20	25.91941822279017	24.698221965730934	24.84065192299765	24.541707888481252
21	26.257130049002182	24.54570359263439	24.607922448425764	24.589243909937668
22	25.89726799420321	24.587794535988404	24.762857602919865	24.75207986688852
23	25.125190759715327	24.446829030343782	25.03776548556667	25.390214724374218
24	25.01433103993755	24.85515451544768	25.076893812039447	25.053620632575324
25	25.281084627685246	24.41687607027717	25.110613199040206	25.191426102997376
26	24.899935201037813	25.111165217600412	25.165572298508078	24.823327282853697
27	25.28859226406976	24.921952765427157	24.800856093352653	24.98859887715043
28	25.14004240026555	24.76950169596768	25.07004803846169	25.02040786530508
29	24.975030165621067	24.967429717323437	25.075467728153004	24.982072388902488
30	25.03064918736176	25.01557687245601	25.04731032178748	24.90646361839475
31	25.102525102009793	24.837312523994097	24.797805468978805	25.26235690501731
32	25.16861825993943	24.878380577649853	24.76621889306036	25.18678226935036
33	24.905775752087063	24.69755272456976	24.97216321353102	25.42450830981216
34	25.324932411310243	24.699586738532254	25.18163923613616	24.793841614021346
35	25.25459464101683	24.690870834764684	25.24781003091721	24.80672449330127
36	24.99478251011506	25.01573835426198	24.98786879923872	25.00161033638424
37	25.418866310601317	24.66246361838314	24.801935429017522	25.11673464199802
38	24.939461593593297	24.716245678358664	24.978882949919846	25.36540977812819
39	25.275124486637544	24.690479584878503	24.908449288979046	25.125946639504903
40	25.478140785945797	24.50468307446028	25.095241694048298	24.921934445545627
41	24.838210874857168	24.75902856531875	25.340872542675356	25.061888017148725
42	25.36442009466528	24.815004227431974	25.094800537779154	24.72577514012359
43	25.01720816012456	24.35447392840289	25.29408455808629	25.33423335338626
44	24.674053765079965	24.5663604334356	25.316263281855523	25.443322519628914
45	24.785751520715483	24.716317751599725	25.29252786153438	25.20540286615042
46	24.721836719270645	24.524228287977575	25.339008814658577	25.414926178093207
47	24.887829699526584	24.423732998400478	25.605230105310618	25.083207196762313
48	24.89343329226824	24.871190296557533	25.11281449899799	25.122561912176238
49	24.66258285141107	24.866630483136014	25.222168023262803	25.248618642190113
50	24.665628671343086	24.961869559541885	25.192455669069	25.180046100046034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	26.0
2	44.0
3	106.5
4	169.0
5	223.0
6	277.0
7	266.5
8	256.0
9	356.5
10	457.0
11	629.0
12	801.0
13	1497.0
14	2193.0
15	2934.5
16	3676.0
17	3728.0
18	3780.0
19	3735.5
20	3691.0
21	4373.0
22	5055.0
23	6212.0
24	7369.0
25	9570.5
26	11772.0
27	15240.5
28	18709.0
29	22312.0
30	25915.0
31	30614.0
32	35313.0
33	41195.5
34	47078.0
35	55123.5
36	63169.0
37	71820.5
38	80472.0
39	90157.5
40	99843.0
41	109646.0
42	119449.0
43	128007.0
44	136565.0
45	147685.0
46	158805.0
47	166634.5
48	174464.0
49	180375.0
50	186286.0
51	185771.0
52	185256.0
53	183989.0
54	182722.0
55	177741.5
56	172761.0
57	165353.5
58	157946.0
59	143364.0
60	128782.0
61	113121.0
62	97460.0
63	84598.5
64	71737.0
65	60810.5
66	49884.0
67	42084.5
68	34285.0
69	29194.0
70	24103.0
71	18565.0
72	13027.0
73	11139.5
74	9252.0
75	7674.0
76	6096.0
77	5165.0
78	4234.0
79	3433.5
80	2633.0
81	2085.0
82	1537.0
83	1176.5
84	816.0
85	587.5
86	359.0
87	265.5
88	172.0
89	132.0
90	92.0
91	67.5
92	43.0
93	37.5
94	32.0
95	22.0
96	12.0
97	12.5
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009017225477011228
2	4.29391689381487E-4
3	3.435133515051896E-4
4	0.0
5	0.0
6	3.005741825670409E-4
7	4.29391689381487E-5
8	0.0
9	0.0012022967302681637
10	7.729050408866766E-4
11	0.0
12	8.58783378762974E-5
13	5.152700272577844E-4
14	2.146958446907435E-4
15	0.0038645252044333836
16	5.582091961959331E-4
17	4.29391689381487E-5
18	3.864525204433383E-4
19	0.0
20	5.152700272577844E-4
21	0.0
22	4.29391689381487E-5
23	0.0012881750681444611
24	6.440875340722306E-4
25	0.001717566757525948
26	0.005796787806650075
27	0.0067843886922274955
28	0.005023882765763398
29	0.0028339851499178145
30	0.004422734400629316
31	0.007643172070990469
32	0.004079221049124127
33	0.005281517779392291
34	0.0036068901908044913
35	0.0032633768392993016
36	0.007299658719485279
37	0.0038645252044333836
38	0.008115502929310105
39	0.004809186921072655
40	0.0027481068120415167
41	0.0031345593324848555
42	0.0016746275885877995
43	0.0016746275885877995
44	0.001760505926464097
45	0.0016316884196496508
46	0.0012022967302681637
47	0.002189897615845584
48	0.0022757759537218813
49	3.435133515051896E-4
50	0.0012022967302681637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2328876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.40657609193811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.94355690340251	28.39514425220118
2	15.919183379340792	14.138328561124268
3	7.416060280188172	9.87965535403728
4	4.00475925811183	7.113505869009465
5	2.456180388756585	5.453528066442271
6	1.5598369125026181	4.156020992763679
7	1.0852241589871223	3.3733762435009833
8	0.7416771213202666	2.634827321884643
9	0.5450943553777788	2.1785196572432364
>10	2.210464850632375	15.95152352455511
>50	0.07583657107203698	2.2918429754868885
>100	0.03803436296873288	3.1793905179642987
>500	0.0037992103865505406	1.1155619356370874
>1k	2.922469528113993E-4	0.1387747281496063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.2939168938148704E-5
2	0.0	0.0	0.0	0.0	4.2939168938148704E-5
3	0.0	0.0	0.0	0.0	4.2939168938148704E-5
4	0.0	0.0	0.0	0.0	4.2939168938148704E-5
5	0.0	0.0	0.0	0.0	8.587833787629741E-5
6	0.0	0.0	0.0	0.0	8.587833787629741E-5
7	0.0	0.0	0.0	0.0	8.587833787629741E-5
8	0.0	0.0	0.0	0.0	8.587833787629741E-5
9	0.0	0.0	0.0	0.0	8.587833787629741E-5
10	0.0	0.0	0.0	0.0	8.587833787629741E-5
11	0.0	0.0	0.0	0.0	8.587833787629741E-5
12	0.0	0.0	0.0	0.0	3.8645252044333835E-4
13	0.0	0.0	0.0	4.2939168938148704E-5	4.2939168938148706E-4
14	0.0	0.0	0.0	4.2939168938148704E-5	4.2939168938148706E-4
15	0.0	0.0	0.0	8.587833787629741E-5	5.152700272577844E-4
16	0.0	0.0	0.0	8.587833787629741E-5	5.152700272577844E-4
17	0.0	0.0	0.0	8.587833787629741E-5	5.152700272577844E-4
18	0.0	0.0	0.0	8.587833787629741E-5	5.582091961959331E-4
19	0.0	0.0	0.0	8.587833787629741E-5	6.011483651340818E-4
20	0.0	0.0	0.0	1.288175068144461E-4	7.29965871948528E-4
21	0.0	0.0	0.0	1.7175667575259482E-4	8.158442098248254E-4
22	0.0	0.0	0.0	2.1469584469074353E-4	8.587833787629741E-4
23	0.0	0.0	0.0	3.005741825670409E-4	8.587833787629741E-4
24	0.0	0.0	0.0	3.8645252044333835E-4	8.587833787629741E-4
25	0.0	0.0	0.0	4.2939168938148706E-4	8.587833787629741E-4
26	0.0	0.0	0.0	6.440875340722306E-4	9.876008855774201E-4
27	0.0	0.0	0.0	0.0012881750681444611	0.0011164183923918663
28	0.0	0.0	0.0	0.00429391689381487	0.0011164183923918663
29	0.0	0.0	0.0	0.011464758106485704	0.0011164183923918663
30	0.0	0.0	0.0	0.024389447956868465	0.0011164183923918663
31	0.0	0.0	0.0	0.06002895817553189	0.001159357561330015
32	0.0	0.0	0.0	0.0979442443479172	0.001159357561330015
33	0.0	0.0	0.0	0.13676125306800363	0.001159357561330015
34	0.0	0.0	0.0	0.1766088018426056	0.0012881750681444611
35	0.0	0.0	0.0	0.22336955681624956	0.0012881750681444611
36	0.0	0.0	0.0	0.297139049051989	0.0012881750681444611
37	0.0	0.0	0.0	0.4150929461250835	0.0012881750681444611
38	0.0	0.0	0.0	0.5763724646567701	0.0012881750681444611
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2655	0.0	28.089106	1
GGTATCA	1080	0.0	24.647018	1
TCAACGC	3760	0.0	19.482313	4
ATCAACG	3775	0.0	19.346626	3
CAACGCA	3850	0.0	19.026882	5
TATCAAC	3935	0.0	18.95171	2
AACGCAG	3990	0.0	18.414406	6
TACCGTC	260	0.0	17.767653	7
CGTTAAG	75	0.0012919409	17.598814	2
ATCGATT	130	7.320905E-7	16.923754	29
GTATAGG	280	0.0	16.499243	1
ACGCAGA	4595	0.0	16.037748	7
AGAGTAC	4700	0.0	15.727613	11
CCTATAC	225	2.5465852E-11	15.643055	3
CGCAGAG	4705	0.0	15.5225315	8
CAGAGTA	4855	0.0	15.27081	10
CGTCGTA	260	1.8189894E-12	15.230723	10
TAAGACT	430	0.0	14.835893	4
CGGATCG	135	1.956377E-5	14.667568	26
ATACCGT	285	0.0	14.665365	6
>>END_MODULE