FastQCFastQC Report
Thu 2 Feb 2017
SRR4063914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050358
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39410.375205406156758No Hit
TATCAACGCAGAGTACTTTTTTTTT29150.27752442500556956No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23570.22439968087071266No Hit
GTACATGGGGTGGTATCAACGCAAA15620.14871120132373916No Hit
GGTATCAACGCAGAGTACTTTTTTT15450.14709270553468434No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14020.13347829977969417No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13800.13138377581738797No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13750.13090774764413657No Hit
GATTAAGAGGGACGGCCGGGGGCAT12380.11786457569704806No Hit
GAATAGGACCGCGGTTCTATTTTGT12300.1171029306198458No Hit
GTATCTGATCGTCTTCGAACCTCCG11530.10977209675177417No Hit
GTACATGGGGAATAATTGCAATCCC11410.1086296291359708No Hit
TTGTAGAACAGTGTATATCAATGAG11350.10805839532806909No Hit
CTATTGGAGCTGGAATTACCGCGGC11090.10558304882716178No Hit
ATCAGATACCGTCGTAGTTCCGACC11090.10558304882716178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA903.6237907E-813.72525216
AACGAAC903.6237907E-813.72525214
ACGAACG903.6237907E-813.72525215
TAACGAA903.6259735E-813.72459813
GTCTTAC852.706165E-713.4076951
CGGTTCT2200.013.38804212
ATAACGA1001.00390025E-813.30166812
TTCATAC655.472069E-513.1479753
AACCGCG1450.013.1025937
TCCAACG2250.013.09178118
CGCGCGC604.0824446E-412.66946414
TATACTA604.0956005E-412.6646335
GGATAGG604.1005434E-412.6628231
GTAGGAC2500.012.5344023
CCCGTTT1750.012.4872812
GACGTGG1750.012.48489957
CGGACAT1600.012.4667485
AGGCCCG2300.012.39226810
CCAACGT853.9301467E-612.29683319
ACATCTA1551.8189894E-1212.2590188
Produced by FastQC (version 0.11.5)