##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063914_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050358
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21308163502349	32.0	32.0	32.0	32.0	32.0
2	30.815358192159245	32.0	32.0	32.0	32.0	32.0
3	30.852384615531086	32.0	32.0	32.0	32.0	32.0
4	30.871824654070327	32.0	32.0	32.0	32.0	32.0
5	30.77930381831718	32.0	32.0	32.0	32.0	32.0
6	34.438293419957766	36.0	36.0	36.0	32.0	36.0
7	34.35946505858003	36.0	36.0	36.0	32.0	36.0
8	34.317422250318465	36.0	36.0	36.0	32.0	36.0
9	34.43954251788438	36.0	36.0	36.0	32.0	36.0
10	34.149945066348806	36.0	36.0	36.0	32.0	36.0
11	34.442723338138045	36.0	36.0	36.0	32.0	36.0
12	34.24911506362593	36.0	36.0	36.0	32.0	36.0
13	34.34284120271374	36.0	36.0	36.0	32.0	36.0
14	34.234147785802556	36.0	36.0	36.0	32.0	36.0
15	34.17383596830795	36.0	36.0	36.0	32.0	36.0
16	34.16575300992614	36.0	36.0	36.0	32.0	36.0
17	34.08556225591656	36.0	36.0	36.0	32.0	36.0
18	34.08692179237936	36.0	36.0	36.0	32.0	36.0
19	34.09688506204551	36.0	36.0	36.0	32.0	36.0
20	34.06183796381805	36.0	36.0	36.0	32.0	36.0
21	34.045387382206826	36.0	36.0	36.0	32.0	36.0
22	34.02499338320839	36.0	36.0	36.0	32.0	36.0
23	33.966557116716395	36.0	36.0	36.0	32.0	36.0
24	33.96210910946554	36.0	36.0	36.0	32.0	36.0
25	33.55550488500111	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	12.0
5	25.0
6	46.0
7	18.0
8	70.0
9	66.0
10	114.0
11	37.0
12	83.0
13	46.0
14	130.0
15	199.0
16	336.0
17	458.0
18	569.0
19	813.0
20	1207.0
21	1892.0
22	2741.0
23	4131.0
24	6020.0
25	8518.0
26	11962.0
27	15871.0
28	21541.0
29	28778.0
30	38026.0
31	50855.0
32	71802.0
33	101564.0
34	215242.0
35	467181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.18409673621932	17.07932202356688	11.41370810744913	25.322873132764673
2	16.310564434969965	20.0600395884251	38.229584302494416	25.399811674110524
3	19.0527013311956	24.50411012116146	28.625370596400764	27.817817951242173
4	12.306769494430164	15.636960868323335	36.094544415881174	35.96172522136533
5	14.288067505020313	37.07786748273955	33.496852603184244	15.137212409055895
6	33.52923476558483	35.86195995383684	17.250720823319256	13.358084457259082
7	29.65929230086498	30.640456575171964	20.917854751880576	18.782396372082484
8	28.13620600815857	33.030109368258536	19.511800209345495	19.3218844142374
9	27.640162166693337	13.913719393703847	18.478658466884614	39.967459972718196
10	16.05873420229823	26.89396176732293	31.375050253506316	25.672253776872527
11	37.47025368911414	20.926160558630478	22.67264292042564	18.930942831829746
12	24.682743534827708	23.6659032186764	29.366897098389515	22.28445614810637
13	29.82825196571327	19.402183077859682	25.51935511838105	25.250209838045993
14	23.720445433619414	19.808133508132748	25.488098141739968	30.983322916507873
15	25.021054516908396	27.462139977287798	22.75755104529484	24.759254460508966
16	25.3374683206616	25.68141542331218	24.42824748947198	24.55286876655424
17	23.426956176278086	25.780817558832243	25.668969367505145	25.123256897384522
18	24.47052904693799	25.116461217866565	26.902374579677197	23.510635155518248
19	25.522062543650115	24.441207044235615	25.656502521583285	24.380227890530985
20	25.453161194174918	24.51115382086614	25.387699018829668	24.64798596612928
21	27.203950063935086	23.921536119035505	24.590329508662204	24.28418430836721
22	25.726024838920537	24.243674498466863	25.38328432502892	24.647016337583686
23	24.622585504882114	23.999111909841698	25.62397386041929	25.7543287248569
24	24.465770941102598	25.10979006253436	25.649092951616147	24.775346044746893
25	24.793499461178662	24.521087399584378	25.807684135403754	24.877729003833206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	187.0
2	421.0
3	655.0
4	655.0
5	655.0
6	1800.5
7	2946.0
8	2946.0
9	2946.0
10	2766.5
11	2587.0
12	2587.0
13	2587.0
14	2567.5
15	2548.0
16	2548.0
17	2548.0
18	4493.0
19	6438.0
20	6438.0
21	6438.0
22	10967.0
23	15496.0
24	15496.0
25	15496.0
26	24197.5
27	32899.0
28	32899.0
29	32899.0
30	42719.0
31	52539.0
32	52539.0
33	52539.0
34	60339.5
35	68140.0
36	68140.0
37	68140.0
38	76622.0
39	85104.0
40	85104.0
41	85104.0
42	97554.0
43	110004.0
44	110004.0
45	110004.0
46	121943.5
47	133883.0
48	133883.0
49	133883.0
50	137764.5
51	141646.0
52	141646.0
53	141646.0
54	129573.5
55	117501.0
56	117501.0
57	117501.0
58	108134.5
59	98768.0
60	98768.0
61	98768.0
62	87410.5
63	76053.0
64	76053.0
65	76053.0
66	62840.0
67	49627.0
68	49627.0
69	49627.0
70	37303.5
71	24980.0
72	24980.0
73	24980.0
74	19188.0
75	13396.0
76	13396.0
77	13396.0
78	10866.5
79	8337.0
80	8337.0
81	8337.0
82	5758.0
83	3179.0
84	3179.0
85	3179.0
86	2477.5
87	1776.0
88	1776.0
89	1776.0
90	1277.5
91	779.0
92	779.0
93	779.0
94	535.5
95	292.0
96	292.0
97	292.0
98	445.0
99	598.0
100	598.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03903431020661527
2	0.004855487367164338
3	0.0022849352316067473
4	0.005521926809716306
5	0.011900704331285143
6	0.01618495789054779
7	0.02656237206742844
8	0.0398911609184678
9	0.05464803428926137
10	0.06473983156219117
11	0.061693251253382174
12	0.06988093583330636
13	0.07035696400655776
14	0.07368916121931761
15	0.06683435552449736
16	0.07406998375791872
17	0.06864326258285271
18	0.08254328524179375
19	0.07797341477858025
20	0.08539945428130218
21	0.08149602326064066
22	0.08340013595364627
23	0.08711315570500724
24	0.08140081762599037
25	0.08044876127948757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1050358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.72667584840818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.97561235242475	36.045856837841015
2	14.730085975797808	14.35496249123766
3	5.045112222989818	7.374946437254006
4	2.215316515253535	4.317800389615368
5	1.1358348902850215	2.7672729258115254
6	0.6635022011857724	1.939815400913067
7	0.4329081665954071	1.4765923134076162
8	0.31119838946989686	1.2130930438597076
9	0.20573298331569134	0.9022215950414695
>10	1.0716196207714448	9.272788440187748
>50	0.09703729764653597	3.3331372027890707
>100	0.10035838665358589	10.28799414087937
>500	0.011564735737909216	3.7731368091195296
>1k	0.004116261872815144	2.9403819720428763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3941	0.375205406156758	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2915	0.27752442500556956	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2357	0.22439968087071266	No Hit
GTACATGGGGTGGTATCAACGCAAA	1562	0.14871120132373916	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1545	0.14709270553468434	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1402	0.13347829977969417	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1380	0.13138377581738797	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1375	0.13090774764413657	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1238	0.11786457569704806	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1230	0.1171029306198458	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1153	0.10977209675177417	No Hit
GTACATGGGGAATAATTGCAATCCC	1141	0.1086296291359708	No Hit
TTGTAGAACAGTGTATATCAATGAG	1135	0.10805839532806909	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1109	0.10558304882716178	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1109	0.10558304882716178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.520563465028115E-5	0.0
6	0.0	0.0	0.0	9.520563465028115E-5	0.0
7	0.0	0.0	0.0	9.520563465028115E-5	0.0
8	0.0	0.0	0.0	9.520563465028115E-5	0.0
9	0.0	0.0	0.0	1.904112693005623E-4	0.0
10	0.0	0.0	0.0	2.856169039508434E-4	0.0
11	0.0	0.0	0.0	2.856169039508434E-4	0.0
12	0.0	0.0	0.0	2.856169039508434E-4	3.808225386011246E-4
13	0.0	0.0	0.0	2.856169039508434E-4	3.808225386011246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	90	3.6237907E-8	13.725252	16
AACGAAC	90	3.6237907E-8	13.725252	14
ACGAACG	90	3.6237907E-8	13.725252	15
TAACGAA	90	3.6259735E-8	13.724598	13
GTCTTAC	85	2.706165E-7	13.407695	1
CGGTTCT	220	0.0	13.388042	12
ATAACGA	100	1.00390025E-8	13.301668	12
TTCATAC	65	5.472069E-5	13.147975	3
AACCGCG	145	0.0	13.102593	7
TCCAACG	225	0.0	13.091781	18
CGCGCGC	60	4.0824446E-4	12.669464	14
TATACTA	60	4.0956005E-4	12.664633	5
GGATAGG	60	4.1005434E-4	12.662823	1
GTAGGAC	250	0.0	12.534402	3
CCCGTTT	175	0.0	12.48728	12
GACGTGG	175	0.0	12.4848995	7
CGGACAT	160	0.0	12.466748	5
AGGCCCG	230	0.0	12.392268	10
CCAACGT	85	3.9301467E-6	12.296833	19
ACATCTA	155	1.8189894E-12	12.259018	8
>>END_MODULE