FastQCFastQC Report
Thu 2 Feb 2017
SRR4063914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050358
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12350.11757895879309721No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12230.11643649117729384No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12200.116150874273343No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11670.1111049756368781No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10800.10282208542230363No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10730.10215564597975167No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10720.10206044034510138No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10630.10120358963324885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC952.687375E-823.155544
ATACCGT3950.020.0485946
TTAGGAC3000.019.7979853
TGTAGAA9100.019.5804252
AAATTCG808.981018E-519.25170921
TTACACA2450.018.8552254
CGGATTA600.007417856218.33059718
TACCGTC4200.018.3305957
CCGTCGT4100.018.2420469
ATACACT6650.018.193644
CGTCGTA4150.018.02226310
TTTAGGA3200.017.8731822
TAGGACG3450.017.8532564
ACCGTCG4200.017.8068648
GTATTAT1509.586074E-917.6007231
TCTAGAT2800.017.2839572
AGGTCGT1154.2720712E-617.2172828
CTAGATA2950.017.1507973
TAGAACA9550.017.0453874
GTCGTAG4400.016.99827211
Produced by FastQC (version 0.11.5)