##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050358
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245897113174745	32.0	32.0	32.0	32.0	32.0
2	31.387687816915758	32.0	32.0	32.0	32.0	32.0
3	31.473631847427257	32.0	32.0	32.0	32.0	32.0
4	31.566577300310943	32.0	32.0	32.0	32.0	32.0
5	31.50300564188591	32.0	32.0	32.0	32.0	32.0
6	35.087011285675935	36.0	36.0	36.0	36.0	36.0
7	35.11255019717087	36.0	36.0	36.0	36.0	36.0
8	35.05825918401155	36.0	36.0	36.0	36.0	36.0
9	35.17289628869395	36.0	36.0	36.0	36.0	36.0
10	35.025188554759424	36.0	36.0	36.0	36.0	36.0
11	35.17245548660552	36.0	36.0	36.0	36.0	36.0
12	35.07078919758787	36.0	36.0	36.0	36.0	36.0
13	35.119229824497936	36.0	36.0	36.0	36.0	36.0
14	35.054845109952986	36.0	36.0	36.0	36.0	36.0
15	35.03421595303696	36.0	36.0	36.0	36.0	36.0
16	35.04043192892328	36.0	36.0	36.0	36.0	36.0
17	35.004237602798284	36.0	36.0	36.0	36.0	36.0
18	35.001921249707244	36.0	36.0	36.0	36.0	36.0
19	34.99585855489271	36.0	36.0	36.0	36.0	36.0
20	34.98583720978942	36.0	36.0	36.0	36.0	36.0
21	34.97972024776314	36.0	36.0	36.0	36.0	36.0
22	34.96459207241721	36.0	36.0	36.0	36.0	36.0
23	34.90839218628315	36.0	36.0	36.0	32.0	36.0
24	34.87400771927286	36.0	36.0	36.0	32.0	36.0
25	34.85329478139834	36.0	36.0	36.0	32.0	36.0
26	34.79916752193062	36.0	36.0	36.0	32.0	36.0
27	34.78962220500058	36.0	36.0	36.0	32.0	36.0
28	34.77048492037953	36.0	36.0	36.0	32.0	36.0
29	34.73666787895175	36.0	36.0	36.0	32.0	36.0
30	34.70089816995729	36.0	36.0	36.0	32.0	36.0
31	34.70051639536234	36.0	36.0	36.0	32.0	36.0
32	34.643830008435216	36.0	36.0	36.0	32.0	36.0
33	34.6180806924877	36.0	36.0	36.0	32.0	36.0
34	34.6019519059216	36.0	36.0	36.0	32.0	36.0
35	34.550514205632744	36.0	36.0	36.0	32.0	36.0
36	34.52402799807304	36.0	36.0	36.0	32.0	36.0
37	34.5207986229457	36.0	36.0	36.0	32.0	36.0
38	34.45327593068268	36.0	36.0	36.0	32.0	36.0
39	34.443171756677245	36.0	36.0	36.0	32.0	36.0
40	34.43082739408849	36.0	36.0	36.0	32.0	36.0
41	34.40130031855805	36.0	36.0	36.0	32.0	36.0
42	34.316188385293394	36.0	36.0	36.0	32.0	36.0
43	34.31630167999863	36.0	36.0	36.0	32.0	36.0
44	34.225564045782484	36.0	36.0	36.0	32.0	36.0
45	34.180186184139124	36.0	36.0	36.0	32.0	36.0
46	34.13627353721303	36.0	36.0	36.0	32.0	36.0
47	34.102995359677365	36.0	36.0	36.0	32.0	36.0
48	34.022733201441795	36.0	36.0	36.0	32.0	36.0
49	34.003942465330866	36.0	36.0	36.0	32.0	36.0
50	33.40252371096331	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	1.0
20	16.0
21	28.0
22	121.0
23	371.0
24	928.0
25	2113.0
26	4332.0
27	7835.0
28	13018.0
29	19493.0
30	27669.0
31	38982.0
32	56411.0
33	90251.0
34	184385.0
35	604398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36699309221958	17.89062760350959	11.912041246732967	25.830338057537862
2	15.610451333550404	20.7172058182289	37.663682910713916	26.008659937506785
3	18.360822425920354	25.054648138345474	28.99388397838447	27.590645457349698
4	11.95087770074584	16.212281907692425	36.2515447114222	35.58529568013953
5	13.884599346127702	37.421431550004854	33.33549132771874	15.358477776148705
6	33.60965234644254	36.27436792858802	16.919501578512428	13.196478146457011
7	29.477025430401284	30.84484608566421	20.914508114859995	18.763620369074516
8	28.068620413230537	33.33073104598623	19.270001275755504	19.330647265027736
9	27.639637718345337	13.676675143262726	18.35486122152478	40.32882591686715
10	15.859810939750732	27.08099086687999	31.448474941281646	25.61072325208763
11	37.631264768774074	20.776249621557604	22.60372177866975	18.988763830998572
12	24.648142770777497	23.702176883053824	29.31275616339238	22.3369241827763
13	29.887761543024105	19.3151254863841	25.60253552853184	25.194577442059956
14	23.65133692894307	19.85452537475425	25.415692789580667	31.078444906722012
15	25.105493668475674	27.53184804341617	22.699990478910788	24.662667809197373
16	25.436759175512925	25.57518922264007	24.427381349074118	24.560670252772884
17	23.575295280275867	25.74103305730046	25.648112357881793	25.03555930454188
18	24.4765331274343	24.995882336700138	27.03005560987592	23.497528925989645
19	25.587705501753693	24.443331594240426	25.68805243174695	24.280910472258928
20	25.632552258870394	24.314036871589227	25.452421483165576	24.600989386374803
21	27.173211419344646	23.916607480497127	24.735851966662796	24.174329133495437
22	25.67193820767606	24.176922703423692	25.587300317891916	24.563838771008335
23	24.65032984337557	23.817081402622183	25.83907984694582	25.69350890705643
24	24.55426804944252	24.9515638105738	25.799756462363533	24.69441167762015
25	24.93440144412031	24.395046118926658	25.91952228256316	24.751030154389866
26	24.232259661617267	25.455064791628978	26.399946681392763	23.912728865360997
27	24.85982376212171	24.58332738256759	25.870692240677528	24.68615661463317
28	24.270996083987193	25.07495456073152	26.01182706323794	24.642222292043346
29	24.05214420775422	24.409750189942475	25.991842470566056	25.546263131737245
30	24.036735855829093	25.432325153835684	26.101556779957136	24.42938221037809
31	24.825924885624104	24.879720832361357	25.153270841168656	25.14108344084588
32	24.65312588961915	25.137745177847666	25.034251824939137	25.17487710759405
33	24.23743288828631	24.626845066319085	25.807935058616533	25.32778698677808
34	25.07554826043823	24.947968337328934	25.81265554753685	24.163827854695988
35	25.41118695093748	24.671788314531	25.97863684444542	23.938387890086098
36	24.03700036181517	25.708110372669623	25.302496524670083	24.952392740845124
37	25.075667919943868	25.347967701056735	25.35453717130036	24.221827207699036
38	24.55513269609248	25.016638784602247	25.626584731165803	24.801643788139476
39	25.14376735205292	24.706276635044198	25.222601370264723	24.927354642638157
40	25.69902239689848	25.2135531512181	25.270202337564406	23.81722211431901
41	24.022819755540702	25.360224236850172	26.515683821471214	24.101272186137912
42	25.75180560924198	25.91956113174997	25.170659349656965	23.157973909351085
43	24.48185480223891	24.75015066650291	25.846945682940554	24.92104884831763
44	24.23521727912439	25.71855919705312	24.920525053530746	25.125698470291745
45	24.45265342650951	25.67111601957461	25.21307386179713	24.663156692118744
46	24.210751807756573	25.335821428401417	24.956609081864357	25.496817681977657
47	24.693786423111234	25.315020341245457	25.38233258340958	24.60886065223373
48	25.411346910018757	25.415726485961553	24.358344520293624	24.814582083726066
49	24.48631408578093	25.95782358261532	24.854762698148235	24.701099633455513
50	24.74203542097877	25.957168073515387	24.355683598993846	24.945112906511998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	46.5
2	37.0
3	97.5
4	158.0
5	200.5
6	243.0
7	230.0
8	217.0
9	307.5
10	398.0
11	603.5
12	809.0
13	1493.0
14	2177.0
15	3004.5
16	3832.0
17	4203.5
18	4575.0
19	4666.5
20	4758.0
21	5065.0
22	5372.0
23	6017.5
24	6663.0
25	6926.5
26	7190.0
27	9556.5
28	11923.0
29	13666.0
30	15409.0
31	17031.0
32	18653.0
33	21523.5
34	24394.0
35	28133.5
36	31873.0
37	36173.0
38	40473.0
39	42955.5
40	45438.0
41	48630.5
42	51823.0
43	52777.0
44	53731.0
45	59753.5
46	65776.0
47	70087.0
48	74398.0
49	77546.0
50	80694.0
51	77314.0
52	73934.0
53	73113.5
54	72293.0
55	73188.0
56	74083.0
57	72119.0
58	70155.0
59	63794.5
60	57434.0
61	50920.5
62	44407.0
63	38383.0
64	32359.0
65	27878.0
66	23397.0
67	20054.5
68	16712.0
69	15140.0
70	13568.0
71	10351.5
72	7135.0
73	6168.5
74	5202.0
75	4038.5
76	2875.0
77	2514.0
78	2153.0
79	1819.5
80	1486.0
81	1196.5
82	907.0
83	760.5
84	614.0
85	437.5
86	261.0
87	185.5
88	110.0
89	89.0
90	68.0
91	56.0
92	44.0
93	40.5
94	37.0
95	33.0
96	29.0
97	20.5
98	12.0
99	12.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008854124022476146
2	3.808225386011246E-4
3	1.904112693005623E-4
4	0.0
5	0.0
6	1.904112693005623E-4
7	9.520563465028115E-5
8	0.0
9	0.0014280845197542171
10	4.760281732514057E-4
11	0.0
12	2.856169039508434E-4
13	4.760281732514057E-4
14	2.856169039508434E-4
15	0.005521926809716306
16	7.616450772022492E-4
17	0.0
18	4.760281732514057E-4
19	1.904112693005623E-4
20	9.520563465028114E-4
21	0.0
22	9.520563465028115E-5
23	0.0010472619811530927
24	6.66439442551968E-4
25	0.0028561690395084343
26	0.006473983156219118
27	0.006950011329470524
28	0.005236309905765463
29	0.0038082253860112457
30	0.004665076097863776
31	0.008854124022476146
32	0.004665076097863776
33	0.005426721175066026
34	0.0034274028474101216
35	0.004284253559262651
36	0.009330152195727552
37	0.004284253559262651
38	0.009615769099678395
39	0.004760281732514057
40	0.003236991578109559
41	0.0038082253860112457
42	0.0019041126930056228
43	0.0023801408662570286
44	0.0025705521355575906
45	0.0017137014237050608
46	0.0021897295969564665
47	0.002760963404858153
48	0.002665757770207872
49	7.616450772022492E-4
50	0.0019041126930056228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1050358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.65775973737641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95819963685308	39.47130866823706
2	14.45464498368693	15.222984452801192
3	4.806719087571148	7.59333176515148
4	2.1339866510799186	4.494838254213395
5	1.0772743138753085	2.836342599564651
6	0.6398094744918952	2.021456015129477
7	0.3731556809330525	1.375467953384684
8	0.2631389876280617	1.108504767044395
9	0.19970580593388992	0.9464454312323307
>10	0.9197008629794697	8.548194532707148
>50	0.0779207409921485	2.88013610373099
>100	0.08445838673520391	9.105262833299472
>500	0.00946516349155706	3.3222188896287324
>1k	0.0018202237483763578	1.0735077338750123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1235	0.11757895879309721	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1223	0.11643649117729384	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1220	0.116150874273343	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1167	0.1111049756368781	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1080	0.10282208542230363	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1073	0.10215564597975167	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1072	0.10206044034510138	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1063	0.10120358963324885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.904112693005623E-4
2	0.0	0.0	0.0	0.0	1.904112693005623E-4
3	0.0	0.0	0.0	0.0	1.904112693005623E-4
4	0.0	0.0	0.0	0.0	1.904112693005623E-4
5	0.0	0.0	0.0	9.520563465028115E-5	1.904112693005623E-4
6	0.0	0.0	0.0	9.520563465028115E-5	1.904112693005623E-4
7	0.0	0.0	0.0	9.520563465028115E-5	1.904112693005623E-4
8	0.0	0.0	0.0	9.520563465028115E-5	1.904112693005623E-4
9	0.0	0.0	0.0	9.520563465028115E-5	1.904112693005623E-4
10	0.0	0.0	0.0	1.904112693005623E-4	1.904112693005623E-4
11	0.0	0.0	0.0	1.904112693005623E-4	1.904112693005623E-4
12	0.0	0.0	0.0	1.904112693005623E-4	1.904112693005623E-4
13	0.0	0.0	0.0	1.904112693005623E-4	1.904112693005623E-4
14	0.0	0.0	0.0	1.904112693005623E-4	1.904112693005623E-4
15	0.0	0.0	0.0	2.856169039508434E-4	2.856169039508434E-4
16	0.0	0.0	0.0	2.856169039508434E-4	2.856169039508434E-4
17	0.0	0.0	0.0	4.760281732514057E-4	2.856169039508434E-4
18	0.0	0.0	0.0	6.66439442551968E-4	2.856169039508434E-4
19	0.0	0.0	0.0	6.66439442551968E-4	2.856169039508434E-4
20	0.0	0.0	0.0	6.66439442551968E-4	2.856169039508434E-4
21	0.0	0.0	0.0	9.520563465028114E-4	2.856169039508434E-4
22	0.0	0.0	0.0	0.001237673250453655	3.808225386011246E-4
23	0.0	0.0	0.0	0.0023801408662570286	3.808225386011246E-4
24	0.0	0.0	0.0	0.004189047924612371	3.808225386011246E-4
25	0.0	0.0	0.0	0.005521926809716306	3.808225386011246E-4
26	0.0	0.0	0.0	0.006759600060169961	3.808225386011246E-4
27	0.0	0.0	0.0	0.00752124513737221	4.760281732514057E-4
28	0.0	0.0	0.0	0.01085344235013205	4.760281732514057E-4
29	0.0	0.0	0.0	0.017137014237050607	4.760281732514057E-4
30	0.0	0.0	0.0	0.029037718568335747	4.760281732514057E-4
31	0.0	0.0	0.0	0.052934332865556315	4.760281732514057E-4
32	0.0	0.0	0.0	0.07921108802903391	4.760281732514057E-4
33	0.0	0.0	0.0	0.09872824313234155	4.760281732514057E-4
34	0.0	0.0	0.0	0.12376732504536549	4.760281732514057E-4
35	0.0	0.0	0.0	0.1534714830562532	4.760281732514057E-4
36	0.0	0.0	0.0	0.19621881301422944	4.760281732514057E-4
37	0.0	0.0	0.0	0.259054531883415	4.760281732514057E-4
38	0.0	0.0	0.0	0.3438827523568155	4.760281732514057E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACC	95	2.687375E-8	23.15554	4
ATACCGT	395	0.0	20.048594	6
TTAGGAC	300	0.0	19.797985	3
TGTAGAA	910	0.0	19.580425	2
AAATTCG	80	8.981018E-5	19.251709	21
TTACACA	245	0.0	18.855225	4
CGGATTA	60	0.0074178562	18.330597	18
TACCGTC	420	0.0	18.330595	7
CCGTCGT	410	0.0	18.242046	9
ATACACT	665	0.0	18.19364	4
CGTCGTA	415	0.0	18.022263	10
TTTAGGA	320	0.0	17.873182	2
TAGGACG	345	0.0	17.853256	4
ACCGTCG	420	0.0	17.806864	8
GTATTAT	150	9.586074E-9	17.600723	1
TCTAGAT	280	0.0	17.283957	2
AGGTCGT	115	4.2720712E-6	17.21728	28
CTAGATA	295	0.0	17.150797	3
TAGAACA	955	0.0	17.045387	4
GTCGTAG	440	0.0	16.998272	11
>>END_MODULE