FastQCFastQC Report
Thu 2 Feb 2017
SRR4063913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696919
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55400.3264740391262046No Hit
TATCAACGCAGAGTACTTTTTTTTT43760.25787913271051827No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33180.19553084148388936No Hit
GCGCAAGACGGACCAGAGCGAAAGC30290.17849997554391223No Hit
GTATCTGATCGTCTTCGAACCTCCG30130.1775570902323564No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG29360.173019454670494No Hit
GAATAGGACCGCGGTTCTATTTTGT29060.17125154471132684No Hit
GTCTTGCGCCGGTCCAAGAATTTCA26510.15622431005840584No Hit
GAACTACGACGGTATCTGATCGTCT26280.15486891242304435No Hit
GATTAAGAGGGACGGCCGGGGGCAT26270.1548099820910721No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25960.1529831417999327No Hit
CTATTGGAGCTGGAATTACCGCGGC25280.14897587922582045No Hit
GTCCTATTCCATTATTCCTAGCTGC24830.14632401428706968No Hit
ATCAGATACCGTCGTAGTTCCGACC24460.14414359200409682No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24060.14178637872520727No Hit
GGGTAGGCACACGCTGAGCCAGTCA24010.14149172706534607No Hit
GAATAACGCCGCCGCATCGCCAGTC23270.1371308824994004No Hit
GTGCATGGCCGTTCTTAGTTGGTGG22200.13082533697837082No Hit
TCGTAGTTCCGACCATAAACGATGC21280.1254037464369248No Hit
CTCTTAATCATGGCCTCAGTTCCGA20900.12316439382197972No Hit
GTACATGGGGAATAATTGCAATCCC20780.12245722983831285No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG20740.1222215085104239No Hit
CTTTAATATACGCTATTGGAGCTGG20480.12068931987914568No Hit
GTCCTGTATTGTTATTTTTCGTCAC19860.11703563929686685No Hit
CATCTAAGGGCATCACAGACCTGTT19190.11308730705472682No Hit
GAATAATGGAATAGGACCGCGGTTC19170.11296944639078235No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT18940.11161404875542084No Hit
GGTATCAACGCAGAGTACTTTTTTT18760.11055330277992055No Hit
GCTTTGAACACTCTAATTTTTTCAA18720.11031758145203159No Hit
GTATCAACGCAGAGTACATGGGGAA18390.10837288049694771No Hit
ATACAGGACTCTTTCGAGGCCCTGT18120.10678176153369726No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC17830.10507278190650231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2300.014.04496116
ATCGCCA3950.013.71030816
CCGTCGT3850.013.5728629
CGTCTTA500.001498120513.30179715
CGTCGTA4000.013.30140310
CGGACAT2450.013.1819545
CGCATCG4050.013.13680213
GCATCGC4050.013.13680214
AACCGCG2250.013.0894987
AGAACCG2400.013.0607975
GCGTTAT1900.012.99753951
CGCCAGT4100.012.97736318
ACTCTAA3800.012.75134610
CGCGGTC2350.012.53323710
CCGCATC4400.012.52367912
TCGCCAG4250.012.51896817
AAGACGG5400.012.4891135
AGGCTTA701.0917406E-412.2134146
GTCCTAA1251.4151738E-912.1576981
TAGAACC3000.012.031414
Produced by FastQC (version 0.11.5)