##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063913_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696919
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.186323566416547	32.0	32.0	32.0	32.0	32.0
2	30.94555131977425	32.0	32.0	32.0	32.0	32.0
3	30.96601841337153	32.0	32.0	32.0	32.0	32.0
4	30.958578458960034	32.0	32.0	32.0	32.0	32.0
5	30.951755505124286	32.0	32.0	32.0	32.0	32.0
6	34.45874257993457	36.0	36.0	36.0	32.0	36.0
7	34.41137673630857	36.0	36.0	36.0	32.0	36.0
8	34.381131332727136	36.0	36.0	36.0	32.0	36.0
9	34.46653493773127	36.0	36.0	36.0	32.0	36.0
10	34.26396545739661	36.0	36.0	36.0	32.0	36.0
11	34.44400822903156	36.0	36.0	36.0	32.0	36.0
12	34.32121863212092	36.0	36.0	36.0	32.0	36.0
13	34.39649505957562	36.0	36.0	36.0	32.0	36.0
14	34.30586845924879	36.0	36.0	36.0	32.0	36.0
15	34.26477221364131	36.0	36.0	36.0	32.0	36.0
16	34.25288478707587	36.0	36.0	36.0	32.0	36.0
17	34.181170108885574	36.0	36.0	36.0	32.0	36.0
18	34.17572258899806	36.0	36.0	36.0	32.0	36.0
19	34.17872037498549	36.0	36.0	36.0	32.0	36.0
20	34.13411247089578	36.0	36.0	36.0	32.0	36.0
21	34.09491319267449	36.0	36.0	36.0	32.0	36.0
22	34.09399800461896	36.0	36.0	36.0	32.0	36.0
23	34.06635378589078	36.0	36.0	36.0	32.0	36.0
24	34.0451636171202	36.0	36.0	36.0	32.0	36.0
25	33.53717236945311	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	15.0
6	61.0
7	32.0
8	94.0
9	99.0
10	161.0
11	49.0
12	100.0
13	99.0
14	224.0
15	416.0
16	731.0
17	962.0
18	1272.0
19	1712.0
20	2267.0
21	3012.0
22	4091.0
23	5921.0
24	7909.0
25	11135.0
26	15669.0
27	21016.0
28	29466.0
29	40614.0
30	56109.0
31	80700.0
32	121616.0
33	183081.0
34	411197.0
35	697073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66961998675929	17.275065540360895	11.370921007874275	25.684393465005545
2	17.140117583826804	19.580194150039603	37.03772677554407	26.24196149058952
3	18.81596611220265	23.88304749736872	28.369126179949955	28.93186021047868
4	12.37756126135842	14.867617948115027	35.66627712569444	37.088543664832116
5	14.77771864655105	36.3079483566332	33.04414038028254	15.87019261653321
6	34.651282311213166	35.46114756745734	16.519223764691738	13.368346356637758
7	30.575930117414064	30.110930353185267	20.683029188475505	18.630110340925164
8	28.682920905456875	32.06568034535591	19.172605443375936	20.078793305811285
9	27.21689441989843	13.77397068863663	18.717683862242016	40.29145102922293
10	15.977226603082286	26.11027975004923	30.803755157681483	27.108738489187
11	38.0870901343327	20.74329618935704	22.143580267368236	19.02603340894203
12	25.04381257886854	23.370207446369392	28.53272086139186	23.05325911337021
13	29.378015032929866	18.702771486375486	25.56470781784292	26.35450566285173
14	23.834315744285387	19.718980934387215	24.91408326787858	31.532620053448817
15	25.16209376319334	26.995127163117388	22.355790208569683	25.486988865119592
16	26.089338365373276	25.387781578016277	23.6335652789244	24.889314777686046
17	23.881920681747555	25.70363298753388	25.082302549064583	25.332143781653976
18	25.152007322300346	24.354846129076773	26.317642521239744	24.175504027383138
19	25.560832981461257	24.363563666043124	25.589965630550832	24.485637721944787
20	25.90228749940287	23.648581304512188	24.70973521576639	25.73939598031855
21	27.663260943282175	23.63347929290695	24.243324929600305	24.459934834210564
22	26.23979797348343	23.631723871931136	24.98503496363275	25.143443190952684
23	24.317884513079655	23.46670724385356	25.93389457244738	26.281513670619404
24	24.897087790896546	24.610290041400788	25.28367204916196	25.208950118540713
25	24.799468749207517	23.97976906395946	25.48977631204143	25.730985874791596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	512.0
3	859.0
4	859.0
5	859.0
6	2539.5
7	4220.0
8	4220.0
9	4220.0
10	4175.0
11	4130.0
12	4130.0
13	4130.0
14	3926.5
15	3723.0
16	3723.0
17	3723.0
18	6751.5
19	9780.0
20	9780.0
21	9780.0
22	16803.5
23	23827.0
24	23827.0
25	23827.0
26	36097.5
27	48368.0
28	48368.0
29	48368.0
30	59721.0
31	71074.0
32	71074.0
33	71074.0
34	87303.5
35	103533.0
36	103533.0
37	103533.0
38	117201.0
39	130869.0
40	130869.0
41	130869.0
42	150977.0
43	171085.0
44	171085.0
45	171085.0
46	192605.5
47	214126.0
48	214126.0
49	214126.0
50	220321.0
51	226516.0
52	226516.0
53	226516.0
54	209467.5
55	192419.0
56	192419.0
57	192419.0
58	180445.0
59	168471.0
60	168471.0
61	168471.0
62	150771.0
63	133071.0
64	133071.0
65	133071.0
66	111125.0
67	89179.0
68	89179.0
69	89179.0
70	67450.5
71	45722.0
72	45722.0
73	45722.0
74	35617.0
75	25512.0
76	25512.0
77	25512.0
78	21653.5
79	17795.0
80	17795.0
81	17795.0
82	11973.0
83	6151.0
84	6151.0
85	6151.0
86	4859.5
87	3568.0
88	3568.0
89	3568.0
90	2509.0
91	1450.0
92	1450.0
93	1450.0
94	937.5
95	425.0
96	425.0
97	425.0
98	653.0
99	881.0
100	881.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037479691134344066
2	0.005126938881584802
3	0.0014732582993059774
4	0.002946516598611955
5	0.007543082492446605
6	0.012787882037975883
7	0.021155989178033838
8	0.03429745320784316
9	0.04626031059820769
10	0.05333195043487638
11	0.05339088076684863
12	0.062407221558601206
13	0.061405405915073145
14	0.06429299218171286
15	0.05816423765659999
16	0.06594304147693555
17	0.061405405915073145
18	0.07525403392854932
19	0.07230751732993737
20	0.0773165955475777
21	0.07330933297346544
22	0.07660943156391083
23	0.08161850978155115
24	0.0765505012319386
25	0.07649157089996635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1696919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.76276606596101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29322751377325	26.472344020058447
2	15.924064881576738	12.345216036474884
3	6.552908198000957	7.620265425924876
4	3.151221456240498	4.886002405211497
5	1.7851857648714247	3.459936909399736
6	1.091340147172855	2.5382017693951706
7	0.6713645848385361	1.8216763842947046
8	0.46370991761485947	1.437974324717668
9	0.32200553660099945	1.1233642758487907
>10	1.4704610271027185	9.804523301716994
>50	0.11333186291736326	3.0758577161464773
>100	0.1260211907804008	10.746112192070466
>500	0.022221022088150633	5.988612417988044
>1k	0.012784697639757899	8.353072445652117
>5k	1.5219878142568927E-4	0.32684037510002917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5540	0.3264740391262046	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4376	0.25787913271051827	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3318	0.19553084148388936	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3029	0.17849997554391223	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3013	0.1775570902323564	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2936	0.173019454670494	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2906	0.17125154471132684	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2651	0.15622431005840584	No Hit
GAACTACGACGGTATCTGATCGTCT	2628	0.15486891242304435	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2627	0.1548099820910721	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2596	0.1529831417999327	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2528	0.14897587922582045	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2483	0.14632401428706968	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2446	0.14414359200409682	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2406	0.14178637872520727	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2401	0.14149172706534607	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2327	0.1371308824994004	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2220	0.13082533697837082	No Hit
TCGTAGTTCCGACCATAAACGATGC	2128	0.1254037464369248	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2090	0.12316439382197972	No Hit
GTACATGGGGAATAATTGCAATCCC	2078	0.12245722983831285	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2074	0.1222215085104239	No Hit
CTTTAATATACGCTATTGGAGCTGG	2048	0.12068931987914568	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1986	0.11703563929686685	No Hit
CATCTAAGGGCATCACAGACCTGTT	1919	0.11308730705472682	No Hit
GAATAATGGAATAGGACCGCGGTTC	1917	0.11296944639078235	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1894	0.11161404875542084	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1876	0.11055330277992055	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1872	0.11031758145203159	No Hit
GTATCAACGCAGAGTACATGGGGAA	1839	0.10837288049694771	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1812	0.10678176153369726	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1783	0.10507278190650231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.178606639444782E-4
2	0.0	0.0	0.0	0.0	1.178606639444782E-4
3	0.0	0.0	0.0	0.0	1.178606639444782E-4
4	0.0	0.0	0.0	0.0	1.178606639444782E-4
5	0.0	0.0	0.0	0.0	1.178606639444782E-4
6	0.0	0.0	0.0	0.0	1.178606639444782E-4
7	0.0	0.0	0.0	0.0	1.178606639444782E-4
8	0.0	0.0	0.0	0.0	1.178606639444782E-4
9	0.0	0.0	0.0	0.0	1.178606639444782E-4
10	0.0	0.0	0.0	0.0	1.178606639444782E-4
11	0.0	0.0	0.0	0.0	1.178606639444782E-4
12	0.0	0.0	0.0	0.0	2.357213278889564E-4
13	0.0	0.0	0.0	0.0	2.946516598611955E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	230	0.0	14.044961	16
ATCGCCA	395	0.0	13.710308	16
CCGTCGT	385	0.0	13.572862	9
CGTCTTA	50	0.0014981205	13.301797	15
CGTCGTA	400	0.0	13.301403	10
CGGACAT	245	0.0	13.181954	5
CGCATCG	405	0.0	13.136802	13
GCATCGC	405	0.0	13.136802	14
AACCGCG	225	0.0	13.089498	7
AGAACCG	240	0.0	13.060797	5
GCGTTAT	190	0.0	12.9975395	1
CGCCAGT	410	0.0	12.977363	18
ACTCTAA	380	0.0	12.751346	10
CGCGGTC	235	0.0	12.533237	10
CCGCATC	440	0.0	12.523679	12
TCGCCAG	425	0.0	12.518968	17
AAGACGG	540	0.0	12.489113	5
AGGCTTA	70	1.0917406E-4	12.213414	6
GTCCTAA	125	1.4151738E-9	12.157698	1
TAGAACC	300	0.0	12.03141	4
>>END_MODULE