FastQCFastQC Report
Thu 2 Feb 2017
SRR4063913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696919
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT28190.166124605829742No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA28040.16524065085015843No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27970.16482813852635275No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27100.15970119964476795No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26200.15439746976726645No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG25960.1529831417999327No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25130.14809192424623685No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA24560.14473289532381922No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA24330.14337749768845773No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23650.1393702351143455No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21100.12434300046142449No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20670.12180899618661821No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19290.11367661037444922No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18990.11190870041528204No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC18720.11031758145203159No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT18480.10890325348469786No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG18040.10631031887791934No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT18010.10613352788200262No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG18000.10607459755003039No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC17530.10330487194733515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG852.3528082E-723.2951621
GTATCAA40950.020.0936991
TCGGCTA550.004486240419.99853912
CGCCCCT4450.019.7750035
ATACCGT7050.018.0990356
TACCGTC7050.018.0985037
AACGCAG44650.017.984076
CGCAATA8500.017.86015136
CGTCGTA6900.017.8548210
GATATAC3850.017.715081
CGGTCCA8600.017.65091310
ACCGTAT750.001291538617.5992348
CCGGTCC8650.017.5483689
CGCGGGA4850.017.2368744
ACCGTCG7150.017.230028
GGTCTAG1154.2731117E-617.2181641
CCGTCGT7200.017.1103679
CCGCCCC5300.017.018634
TCCCCGC5050.016.9908621
TAGCGGC8750.016.84597430
Produced by FastQC (version 0.11.5)