##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696919
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168319171392387	32.0	32.0	32.0	32.0	32.0
2	31.142890143843047	32.0	32.0	32.0	32.0	32.0
3	31.307662298554025	32.0	32.0	32.0	32.0	32.0
4	31.437531785547808	32.0	32.0	32.0	32.0	32.0
5	31.323955946041032	32.0	32.0	32.0	32.0	32.0
6	34.900905700272084	36.0	36.0	36.0	36.0	36.0
7	34.930119233740676	36.0	36.0	36.0	36.0	36.0
8	34.85402721049149	36.0	36.0	36.0	32.0	36.0
9	35.01862080629659	36.0	36.0	36.0	36.0	36.0
10	34.811247325299554	36.0	36.0	36.0	32.0	36.0
11	35.0217317385214	36.0	36.0	36.0	36.0	36.0
12	34.88142392182538	36.0	36.0	36.0	32.0	36.0
13	34.95147381813746	36.0	36.0	36.0	36.0	36.0
14	34.88244164865854	36.0	36.0	36.0	32.0	36.0
15	34.83230077569996	36.0	36.0	36.0	32.0	36.0
16	34.847091110418354	36.0	36.0	36.0	32.0	36.0
17	34.78754495647701	36.0	36.0	36.0	32.0	36.0
18	34.805548172894525	36.0	36.0	36.0	32.0	36.0
19	34.80606617051256	36.0	36.0	36.0	32.0	36.0
20	34.79466256197261	36.0	36.0	36.0	32.0	36.0
21	34.78281933315615	36.0	36.0	36.0	32.0	36.0
22	34.77103444536834	36.0	36.0	36.0	32.0	36.0
23	34.71015175149786	36.0	36.0	36.0	32.0	36.0
24	34.67580656472112	36.0	36.0	36.0	32.0	36.0
25	34.6422268829567	36.0	36.0	36.0	32.0	36.0
26	34.56190542978186	36.0	36.0	36.0	32.0	36.0
27	34.57804998352897	36.0	36.0	36.0	32.0	36.0
28	34.54506667672411	36.0	36.0	36.0	32.0	36.0
29	34.498562394551534	36.0	36.0	36.0	32.0	36.0
30	34.48678281049361	36.0	36.0	36.0	32.0	36.0
31	34.4894806410913	36.0	36.0	36.0	32.0	36.0
32	34.441884379867275	36.0	36.0	36.0	32.0	36.0
33	34.39404650428217	36.0	36.0	36.0	32.0	36.0
34	34.388013216894855	36.0	36.0	36.0	32.0	36.0
35	34.34139048475502	36.0	36.0	36.0	32.0	36.0
36	34.30975668255232	36.0	36.0	36.0	32.0	36.0
37	34.308644077884686	36.0	36.0	36.0	32.0	36.0
38	34.249930020230785	36.0	36.0	36.0	32.0	36.0
39	34.25679658251219	36.0	36.0	36.0	32.0	36.0
40	34.226258295180855	36.0	36.0	36.0	32.0	36.0
41	34.195663434730825	36.0	36.0	36.0	32.0	36.0
42	34.11174251687912	36.0	36.0	36.0	32.0	36.0
43	34.11375439841265	36.0	36.0	36.0	32.0	36.0
44	34.01443321690664	36.0	36.0	36.0	32.0	36.0
45	33.9864495594663	36.0	36.0	36.0	32.0	36.0
46	33.96169646282468	36.0	36.0	36.0	32.0	36.0
47	33.926662380467185	36.0	36.0	36.0	32.0	36.0
48	33.873999289300194	36.0	36.0	36.0	32.0	36.0
49	33.8433814460207	36.0	36.0	36.0	32.0	36.0
50	33.13861474825846	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	8.0
20	11.0
21	59.0
22	196.0
23	530.0
24	1542.0
25	3426.0
26	7116.0
27	13197.0
28	22209.0
29	35170.0
30	52216.0
31	75607.0
32	111922.0
33	182355.0
34	358826.0
35	832525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57125934552175	18.302194363278684	12.007293720775907	26.119252570423658
2	16.277381338937236	20.456145954447873	36.38607282754036	26.880399879074528
3	18.161412118844183	24.545777592342795	28.58629592241432	28.70651436639871
4	12.083605640575655	15.618895185922252	35.82451490023979	36.472984273262306
5	14.523557105554245	36.68772640296915	32.62618899311045	16.162527498366156
6	34.809542498912734	35.86251277318709	16.168291380706385	13.159653347193789
7	30.5179743511472	30.315725332632454	20.50046024616393	18.66584007005642
8	28.728242184806703	32.321578107145946	18.89312336063183	20.05705634741552
9	27.38165885337631	13.490758119977961	18.40874067045987	40.71884235618586
10	16.028954009021167	26.255202139433248	30.715635235588707	27.000208615956883
11	38.44744504599218	20.638698723981523	21.904522254745217	19.009333975281084
12	25.0558218885448	23.506657669948112	28.205891279174267	23.23162916233282
13	29.511877471403903	18.761395713384918	25.416610191465665	26.310116623745515
14	23.946705607944775	19.66558116675329	24.723115019382245	31.664598205919685
15	25.306067124377524	27.052302796357957	22.104723458172497	25.536906621092026
16	26.34885594386473	25.41916116497073	23.340725239716402	24.89125765144814
17	24.062197429576777	25.867410288882382	24.703889814422492	25.366502467118345
18	25.35825306512112	24.400927565611713	25.98503755344257	24.255781815824598
19	25.835956716824267	24.477670694753666	25.122427836819455	24.563944751602612
20	26.284149752990892	23.536670672007382	24.381504872706188	25.797674702295538
21	27.91028917703202	23.61686091086257	23.947577933890777	24.525271978214636
22	26.463034748880027	23.737269567533613	24.698895291345842	25.10080039224052
23	24.640758367572076	23.381291148371037	25.60227189688539	26.375678587171492
24	25.136188558982436	24.70316669338186	24.97524915847139	25.185395589164315
25	25.197846655214377	24.012976642632573	25.182170965204094	25.607005736948956
26	24.844400938456378	25.48771856209435	25.54865575729788	24.119224742151392
27	25.655340435212054	24.181767391862934	24.74187130339669	25.42102086952832
28	24.572120943532106	24.386955184228736	25.75513095379814	25.285792918441025
29	24.475083520489555	24.44349630411889	25.65860230103442	25.422817874357133
30	24.24823528441122	24.849929929977122	25.947065014844945	24.954769770766717
31	25.312765205091935	24.620815652993873	24.51049033474776	25.555928807166435
32	25.09816609296298	24.591350304590396	24.159967563789543	26.15051603865708
33	24.370645257673452	24.100498745622055	25.216385352448672	26.31247064425582
34	25.60049879749965	24.326867941829743	25.23948349943219	24.833149761238413
35	26.225915285451194	24.014615101289134	25.41082872928177	24.3486408839779
36	24.411953295725375	25.251413834479408	24.735384876509016	25.6012479932862
37	25.877396401721526	25.006084743235625	24.566215266134588	24.550303588908264
38	24.705532904996126	24.53297218165552	25.13092337667389	25.630571536674456
39	25.810339269623555	24.42235647089815	24.151619676683456	25.615684582794835
40	25.952851142773127	24.896383423469295	24.885834657822205	24.26493077593538
41	24.42227692401983	25.36995904829873	25.591247921917738	24.616516105763704
42	26.527141321565967	25.580707362148296	24.389650279097836	23.5025010371879
43	25.262478637515468	24.177735871294715	25.05781130296423	25.50197418822559
44	24.758030488394187	25.107253899445343	24.293833254366177	25.840882357794293
45	24.930255253397075	25.461753242084693	24.50477399401969	25.103217510498542
46	24.556178321878193	25.027152310465677	24.523884309647716	25.892785058008418
47	25.16229671658538	24.714713723678347	25.082032327337274	25.040957232399
48	26.399016081782605	25.365890167241346	23.65163543402073	24.583458316955323
49	25.026828096869906	25.750730001208073	23.988649991307756	25.23379191061426
50	25.279273970180917	25.724026165360364	23.838764806411692	25.157935058047027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	22.0
2	16.0
3	76.0
4	136.0
5	167.0
6	198.0
7	197.0
8	196.0
9	305.5
10	415.0
11	641.5
12	868.0
13	1585.5
14	2303.0
15	3326.5
16	4350.0
17	4885.0
18	5420.0
19	5827.5
20	6235.0
21	6807.5
22	7380.0
23	8456.0
24	9532.0
25	10069.5
26	10607.0
27	13137.5
28	15668.0
29	17527.5
30	19387.0
31	21616.5
32	23846.0
33	27445.5
34	31045.0
35	35929.0
36	40813.0
37	50504.5
38	60196.0
39	65858.0
40	71520.0
41	78084.5
42	84649.0
43	85271.5
44	85894.0
45	95843.5
46	105793.0
47	114377.5
48	122962.0
49	127387.5
50	131813.0
51	125214.5
52	118616.0
53	117492.5
54	116369.0
55	120149.5
56	123930.0
57	122542.5
58	121155.0
59	110931.5
60	100708.0
61	89045.0
62	77382.0
63	67533.5
64	57685.0
65	49705.5
66	41726.0
67	36535.5
68	31345.0
69	28792.5
70	26240.0
71	19930.5
72	13621.0
73	11701.5
74	9782.0
75	7566.0
76	5350.0
77	4800.5
78	4251.0
79	3589.0
80	2927.0
81	2402.0
82	1877.0
83	1604.0
84	1331.0
85	963.5
86	596.0
87	428.0
88	260.0
89	208.0
90	156.0
91	141.5
92	127.0
93	101.5
94	76.0
95	60.5
96	45.0
97	43.0
98	41.0
99	47.0
100	53.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006894848840751974
2	3.535819918334346E-4
3	4.714426557779128E-4
4	0.0
5	0.0
6	2.946516598611955E-4
7	5.89303319722391E-5
8	0.0
9	0.0014143279673337384
10	0.001237536971417021
11	0.0
12	2.357213278889564E-4
13	5.303729877501519E-4
14	2.946516598611955E-4
15	0.004066192906084498
16	0.0010607459755003039
17	0.0
18	2.357213278889564E-4
19	5.89303319722391E-5
20	0.0010607459755003039
21	0.0
22	5.89303319722391E-5
23	6.4823365169463E-4
24	4.125123238056737E-4
25	0.0014143279673337384
26	0.005303729877501519
27	0.005362660209473758
28	0.003594750250306585
29	0.001767909959167173
30	0.0026518649387507593
31	0.007012709504696452
32	0.002946516598611955
33	0.003653680582278824
34	0.0022393526149450857
35	0.00259293460677852
36	0.006776988176807496
37	0.0030643772625564333
38	0.007366291496529888
39	0.004007262574112259
40	0.0022393526149450857
41	0.002475073942834042
42	0.0013553976353614995
43	0.0011196763074725429
44	6.4823365169463E-4
45	0.0010018156435280647
46	5.89303319722391E-4
47	0.0015911189632504558
48	0.001767909959167173
49	2.357213278889564E-4
50	0.0011196763074725429
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1696919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.4127400210715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85901524442978	29.629122516900708
2	15.528107118611459	13.171791404820349
3	6.090510240062862	7.749456822223798
4	2.983239864109399	5.061095071078744
5	1.6738122977562422	3.5495482914403906
6	1.000372331730243	2.545711897796874
7	0.6042045841838014	1.7938180363969027
8	0.43085284107526073	1.4618919628692053
9	0.2873566052987011	1.096882289448432
>10	1.3129003131773005	9.540414025335965
>50	0.09444549313700183	2.8413241744291615
>100	0.10644472197648024	9.597668057896856
>500	0.01925190065199844	5.552849177723058
>1k	0.009486443799535463	6.408426271639554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2819	0.166124605829742	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2804	0.16524065085015843	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2797	0.16482813852635275	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2710	0.15970119964476795	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2620	0.15439746976726645	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2596	0.1529831417999327	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2513	0.14809192424623685	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2456	0.14473289532381922	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2433	0.14337749768845773	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2365	0.1393702351143455	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2110	0.12434300046142449	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2067	0.12180899618661821	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1929	0.11367661037444922	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1899	0.11190870041528204	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	1872	0.11031758145203159	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1848	0.10890325348469786	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1804	0.10631031887791934	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1801	0.10613352788200262	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1800	0.10607459755003039	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1753	0.10330487194733515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.89303319722391E-5
2	0.0	0.0	0.0	0.0	5.89303319722391E-5
3	0.0	0.0	0.0	0.0	5.89303319722391E-5
4	0.0	0.0	0.0	0.0	5.89303319722391E-5
5	0.0	0.0	0.0	0.0	5.89303319722391E-5
6	0.0	0.0	0.0	0.0	1.767909959167173E-4
7	0.0	0.0	0.0	0.0	2.357213278889564E-4
8	0.0	0.0	0.0	0.0	2.357213278889564E-4
9	0.0	0.0	0.0	0.0	2.357213278889564E-4
10	0.0	0.0	0.0	0.0	2.357213278889564E-4
11	0.0	0.0	0.0	0.0	2.357213278889564E-4
12	0.0	0.0	0.0	0.0	5.303729877501519E-4
13	0.0	0.0	0.0	0.0	5.303729877501519E-4
14	0.0	0.0	0.0	0.0	5.303729877501519E-4
15	0.0	0.0	0.0	0.0	5.303729877501519E-4
16	0.0	0.0	0.0	0.0	5.303729877501519E-4
17	0.0	0.0	0.0	0.0	5.89303319722391E-4
18	0.0	0.0	0.0	0.0	6.482336516946301E-4
19	0.0	0.0	0.0	0.0	6.482336516946301E-4
20	0.0	0.0	0.0	5.89303319722391E-5	8.839549795835865E-4
21	0.0	0.0	0.0	5.89303319722391E-5	8.839549795835865E-4
22	0.0	0.0	0.0	5.89303319722391E-5	8.839549795835865E-4
23	0.0	0.0	0.0	1.767909959167173E-4	8.839549795835865E-4
24	0.0	0.0	0.0	2.946516598611955E-4	8.839549795835865E-4
25	0.0	0.0	0.0	4.125123238056737E-4	8.839549795835865E-4
26	0.0	0.0	0.0	4.714426557779128E-4	0.0010018156435280647
27	0.0	0.0	0.0	8.250246476113474E-4	0.0011196763074725429
28	0.0	0.0	0.0	0.0016500492952226948	0.0011196763074725429
29	5.89303319722391E-5	0.0	0.0	0.0034179592543898676	0.0011196763074725429
30	5.89303319722391E-5	0.0	0.0	0.00654126684891854	0.0011196763074725429
31	5.89303319722391E-5	0.0	0.0	0.016559423284199185	0.001178606639444782
32	5.89303319722391E-5	0.0	0.0	0.023925714780729076	0.001178606639444782
33	5.89303319722391E-5	0.0	0.0	0.0327063342445927	0.001178606639444782
34	5.89303319722391E-5	0.0	0.0	0.04307807267170678	0.0013553976353614992
35	5.89303319722391E-5	0.0	0.0	0.05680884002123849	0.0013553976353614992
36	5.89303319722391E-5	0.0	0.0	0.07784696853532785	0.0013553976353614992
37	5.89303319722391E-5	0.0	0.0	0.10760678618130859	0.0013553976353614992
38	5.89303319722391E-5	0.0	0.0	0.15145095316865448	0.0014143279673337384
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	85	2.3528082E-7	23.295162	1
GTATCAA	4095	0.0	20.093699	1
TCGGCTA	55	0.0044862404	19.998539	12
CGCCCCT	445	0.0	19.775003	5
ATACCGT	705	0.0	18.099035	6
TACCGTC	705	0.0	18.098503	7
AACGCAG	4465	0.0	17.98407	6
CGCAATA	850	0.0	17.860151	36
CGTCGTA	690	0.0	17.85482	10
GATATAC	385	0.0	17.71508	1
CGGTCCA	860	0.0	17.650913	10
ACCGTAT	75	0.0012915386	17.599234	8
CCGGTCC	865	0.0	17.548368	9
CGCGGGA	485	0.0	17.23687	44
ACCGTCG	715	0.0	17.23002	8
GGTCTAG	115	4.2731117E-6	17.218164	1
CCGTCGT	720	0.0	17.110367	9
CCGCCCC	530	0.0	17.01863	4
TCCCCGC	505	0.0	16.990862	1
TAGCGGC	875	0.0	16.845974	30
>>END_MODULE