FastQCFastQC Report
Thu 2 Feb 2017
SRR4063912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84986
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG2340.27533946767702916No Hit
GTATTGGAATTAGTGAAATTGGAGT1150.13531640505495024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGAAA250.005979829519.00353211
GGTATCA359.830012E-518.9923481
GTATAGT350.00215336116.2791561
GTATCAA1500.014.5608011
GAGAGAT600.0057904811.0853957
TATCAAC2355.456968E-129.2941282
TCAACGC2502.3646862E-118.736484
ATCAACG2502.3646862E-118.736483
CAACGCA2659.094947E-128.6003095
AACGCAG2605.2750693E-118.4004626
CATGGGG1751.6956976E-47.05430134
AGTACAT3153.0395313E-96.93779813
GCAGAGT3153.0395313E-96.9377989
ACGCAGA3204.2200554E-96.82939537
CAGAGTA3204.2200554E-96.829395310
AGAGTAC3255.826223E-96.724327611
CGCAGAG3255.826223E-96.72432768
GAGTACA3152.2399036E-86.636154712
GTACATG4453.0649971E-95.7617241
TACATGG4351.1172233E-85.6758742
Produced by FastQC (version 0.11.5)