##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063912_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84986
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338514578871813	32.0	32.0	32.0	32.0	32.0
2	31.01803826512602	32.0	32.0	32.0	32.0	32.0
3	31.031416939260584	32.0	32.0	32.0	32.0	32.0
4	31.068081801708516	32.0	32.0	32.0	32.0	32.0
5	31.03938295719295	32.0	32.0	32.0	32.0	32.0
6	34.68303014614172	36.0	36.0	36.0	32.0	36.0
7	34.64130562680912	36.0	36.0	36.0	32.0	36.0
8	34.60506436354223	36.0	36.0	36.0	32.0	36.0
9	34.68500694231991	36.0	36.0	36.0	32.0	36.0
10	34.54464264702422	36.0	36.0	36.0	32.0	36.0
11	34.72515473136752	36.0	36.0	36.0	32.0	36.0
12	34.56544607347092	36.0	36.0	36.0	32.0	36.0
13	34.62444402607488	36.0	36.0	36.0	32.0	36.0
14	34.53760619396136	36.0	36.0	36.0	32.0	36.0
15	34.49285764714188	36.0	36.0	36.0	32.0	36.0
16	34.48296189960699	36.0	36.0	36.0	32.0	36.0
17	34.47070105664462	36.0	36.0	36.0	32.0	36.0
18	34.436718989009954	36.0	36.0	36.0	32.0	36.0
19	34.45148612712682	36.0	36.0	36.0	32.0	36.0
20	34.40932624196927	36.0	36.0	36.0	32.0	36.0
21	34.415786129480146	36.0	36.0	36.0	32.0	36.0
22	34.36567199303415	36.0	36.0	36.0	32.0	36.0
23	34.31699338714612	36.0	36.0	36.0	32.0	36.0
24	34.34162097286612	36.0	36.0	36.0	32.0	36.0
25	33.95835784717483	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	2.0
7	1.0
8	4.0
9	8.0
10	10.0
11	3.0
12	9.0
13	7.0
14	10.0
15	7.0
16	31.0
17	32.0
18	43.0
19	43.0
20	55.0
21	113.0
22	145.0
23	228.0
24	360.0
25	504.0
26	750.0
27	987.0
28	1460.0
29	1954.0
30	2494.0
31	3437.0
32	4882.0
33	7093.0
34	16678.0
35	43634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49173072169317	18.96107259308088	13.403882146598708	25.143314538627244
2	15.121847898991565	21.404280856171233	38.93719920454679	24.536672040290412
3	18.181818181818183	26.292565834372724	28.905937448520934	26.619678535288166
4	11.8847298871539	17.788263535059954	37.147429485897185	33.179577091888966
5	13.395074077124935	37.74461925887572	34.01075559844197	14.849551065557373
6	30.4814470478858	38.04267237828487	18.49764042695915	12.978240146870181
7	28.528553269620073	31.69224612777176	21.742149616308083	18.03705098630008
8	27.699298394311818	32.621132928379716	20.238263408202663	19.441305269105804
9	27.752790467668255	15.076767296189894	19.974803372109452	37.195638864032404
10	15.188159382064809	28.872692162773173	33.311746420497364	22.627402034664655
11	34.370290128108515	23.286784099472495	22.986529766390355	19.356396006028636
12	23.251377702416278	26.732136969525694	28.62677217276624	21.38971315529179
13	28.335766767143934	22.263564431047474	26.03146194423512	23.369206857573474
14	23.0723941406434	20.972869860110215	27.309123451556687	28.645612547689698
15	24.781866352664114	28.739476008242566	23.11569031498381	23.36296732410951
16	23.062067991003616	29.1169647798594	24.736526029461984	23.084441199675
17	22.29208614457122	28.51390017308983	26.029413496296822	23.16460018604213
18	22.59735977483896	28.377121188925656	26.40107398989602	22.624445046339368
19	24.79539325710383	26.665410567717473	26.555894440584556	21.983301734594143
20	25.48165245654529	25.927975882048145	26.238871355221633	22.351500306184935
21	26.74508972728557	25.794828317083514	25.180161085205594	22.27992087042532
22	25.09774825701903	26.017524024872806	26.10702845298662	22.777699265121537
23	24.5445230888813	26.600793772302765	25.53968272661964	23.315000412196298
24	23.906489223884112	26.13473089153221	26.244258626781296	23.714521257802378
25	23.073480396198192	25.7458160104585	27.37936801441577	23.80133557892753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	7.0
3	10.0
4	10.0
5	10.0
6	22.0
7	34.0
8	34.0
9	34.0
10	60.0
11	86.0
12	86.0
13	86.0
14	136.5
15	187.0
16	187.0
17	187.0
18	478.5
19	770.0
20	770.0
21	770.0
22	1103.5
23	1437.0
24	1437.0
25	1437.0
26	2205.0
27	2973.0
28	2973.0
29	2973.0
30	4061.0
31	5149.0
32	5149.0
33	5149.0
34	6032.5
35	6916.0
36	6916.0
37	6916.0
38	7630.0
39	8344.0
40	8344.0
41	8344.0
42	9385.5
43	10427.0
44	10427.0
45	10427.0
46	11823.0
47	13219.0
48	13219.0
49	13219.0
50	12819.0
51	12419.0
52	12419.0
53	12419.0
54	10930.5
55	9442.0
56	9442.0
57	9442.0
58	7850.0
59	6258.0
60	6258.0
61	6258.0
62	4910.5
63	3563.0
64	3563.0
65	3563.0
66	2741.0
67	1919.0
68	1919.0
69	1919.0
70	1483.0
71	1047.0
72	1047.0
73	1047.0
74	646.0
75	245.0
76	245.0
77	245.0
78	224.0
79	203.0
80	203.0
81	203.0
82	126.5
83	50.0
84	50.0
85	50.0
86	39.5
87	29.0
88	29.0
89	29.0
90	18.0
91	7.0
92	7.0
93	7.0
94	7.0
95	7.0
96	7.0
97	7.0
98	124.0
99	241.0
100	241.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0388299249288118
2	0.003529993175346528
3	0.0
4	0.003529993175346528
5	0.00823665074247523
6	0.015296637093168287
7	0.025886616619207874
8	0.044713246887722685
9	0.0635398771562375
10	0.0682465347233662
11	0.0682465347233662
12	0.0729531922904949
13	0.0682465347233662
14	0.0729531922904949
15	0.07177652789871274
16	0.07412985668227709
17	0.06942319911514838
18	0.08118984303297014
19	0.07883651424940578
20	0.08236650742475231
21	0.0729531922904949
22	0.08707316499188102
23	0.08824982938366319
24	0.08942649377544537
25	0.0929564869507919
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	84986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.13486927258607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.16477272727273	18.610124020426895
2	14.691051136363637	9.735721177605724
3	7.613636363636364	7.568305367942956
4	4.815340909090909	6.382227661026523
5	3.5546875	5.88920528086979
6	2.723721590909091	5.415009530981574
7	1.960227272727273	4.5466312098463275
8	1.6015624999999998	4.24540512555009
9	1.0511363636363635	3.1346339397077165
>10	5.7350852272727275	32.31826418468924
>50	0.08167613636363635	1.7438166286211847
>100	0.007102272727272727	0.41065587273197934
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	234	0.27533946767702916	No Hit
GTATTGGAATTAGTGAAATTGGAGT	115	0.13531640505495024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGAAA	25	0.0059798295	19.003532	11
GGTATCA	35	9.830012E-5	18.992348	1
GTATAGT	35	0.002153361	16.279156	1
GTATCAA	150	0.0	14.560801	1
GAGAGAT	60	0.00579048	11.085395	7
TATCAAC	235	5.456968E-12	9.294128	2
TCAACGC	250	2.3646862E-11	8.73648	4
ATCAACG	250	2.3646862E-11	8.73648	3
CAACGCA	265	9.094947E-12	8.600309	5
AACGCAG	260	5.2750693E-11	8.400462	6
CATGGGG	175	1.6956976E-4	7.0543013	4
AGTACAT	315	3.0395313E-9	6.937798	13
GCAGAGT	315	3.0395313E-9	6.937798	9
ACGCAGA	320	4.2200554E-9	6.8293953	7
CAGAGTA	320	4.2200554E-9	6.8293953	10
AGAGTAC	325	5.826223E-9	6.7243276	11
CGCAGAG	325	5.826223E-9	6.7243276	8
GAGTACA	315	2.2399036E-8	6.6361547	12
GTACATG	445	3.0649971E-9	5.761724	1
TACATGG	435	1.1172233E-8	5.675874	2
>>END_MODULE