FastQCFastQC Report
Thu 2 Feb 2017
SRR4063912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84986
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT970.11413644600287107No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT860.10119313769326713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTAC207.793192E-444.0253261
GTAGACT207.8158465E-443.9994134
GGTATCA751.8189894E-1235.220261
TAGGTCC250.002340693335.199534
GTTTGGG353.193187E-431.4281520
GCCCAGA300.00569931429.3502161
TGTTTGG300.00571575529.33294119
TGCATCT300.00571575529.33294130
GTATCAA2000.028.616461
AAACCAC450.001387662524.44411719
GTTTCGT500.00256112221.99970611
TATAGTA500.00256112221.9997062
GAATTTT500.00256112221.99970620
TATCAAC2700.020.3700982
CTTCTAG654.8615207E-420.319381
GGAATTT550.004450321719.99973319
ATAGTAG550.004450321719.9997333
CCCTACA600.007357512618.3330882
ATGGAAT600.007357512618.33308817
AAAGCTC600.007357512618.33308840
Produced by FastQC (version 0.11.5)