##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84986
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34875155908032	32.0	32.0	32.0	32.0	32.0
2	31.499929400136494	32.0	32.0	32.0	32.0	32.0
3	31.54353658249594	32.0	32.0	32.0	32.0	32.0
4	31.594721483538464	32.0	32.0	32.0	32.0	32.0
5	31.59794554397195	32.0	32.0	32.0	32.0	32.0
6	35.15451956792884	36.0	36.0	36.0	36.0	36.0
7	35.170839903042854	36.0	36.0	36.0	36.0	36.0
8	35.14980114371779	36.0	36.0	36.0	36.0	36.0
9	35.20012707975431	36.0	36.0	36.0	36.0	36.0
10	35.119478502341565	36.0	36.0	36.0	36.0	36.0
11	35.21756524604052	36.0	36.0	36.0	36.0	36.0
12	35.15187207304732	36.0	36.0	36.0	36.0	36.0
13	35.17346386463653	36.0	36.0	36.0	36.0	36.0
14	35.130492081048644	36.0	36.0	36.0	36.0	36.0
15	35.12262019626762	36.0	36.0	36.0	36.0	36.0
16	35.12294966229732	36.0	36.0	36.0	36.0	36.0
17	35.11768997246605	36.0	36.0	36.0	36.0	36.0
18	35.08835572917892	36.0	36.0	36.0	36.0	36.0
19	35.07750688348669	36.0	36.0	36.0	36.0	36.0
20	35.07384745722825	36.0	36.0	36.0	36.0	36.0
21	35.09326241969266	36.0	36.0	36.0	36.0	36.0
22	35.069776198432685	36.0	36.0	36.0	36.0	36.0
23	35.02721624738192	36.0	36.0	36.0	36.0	36.0
24	35.039147624314595	36.0	36.0	36.0	36.0	36.0
25	34.97509001482597	36.0	36.0	36.0	36.0	36.0
26	34.94208457863648	36.0	36.0	36.0	36.0	36.0
27	34.928529404843154	36.0	36.0	36.0	36.0	36.0
28	34.93702492175182	36.0	36.0	36.0	36.0	36.0
29	34.898324429906104	36.0	36.0	36.0	36.0	36.0
30	34.88772268373614	36.0	36.0	36.0	36.0	36.0
31	34.86839008777916	36.0	36.0	36.0	32.0	36.0
32	34.84928105805662	36.0	36.0	36.0	32.0	36.0
33	34.86105946861836	36.0	36.0	36.0	36.0	36.0
34	34.80052008566117	36.0	36.0	36.0	32.0	36.0
35	34.79699009248582	36.0	36.0	36.0	32.0	36.0
36	34.76229025957217	36.0	36.0	36.0	32.0	36.0
37	34.74486385992987	36.0	36.0	36.0	32.0	36.0
38	34.73736850775422	36.0	36.0	36.0	32.0	36.0
39	34.71841244440261	36.0	36.0	36.0	32.0	36.0
40	34.712493822511945	36.0	36.0	36.0	32.0	36.0
41	34.69923281481656	36.0	36.0	36.0	32.0	36.0
42	34.664509448615064	36.0	36.0	36.0	32.0	36.0
43	34.663497517238135	36.0	36.0	36.0	32.0	36.0
44	34.62223189701833	36.0	36.0	36.0	32.0	36.0
45	34.62011390111312	36.0	36.0	36.0	32.0	36.0
46	34.5772480173205	36.0	36.0	36.0	32.0	36.0
47	34.58912056103358	36.0	36.0	36.0	32.0	36.0
48	34.59781611088885	36.0	36.0	36.0	32.0	36.0
49	34.5683995010943	36.0	36.0	36.0	32.0	36.0
50	33.9825030004942	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	1.0
21	3.0
22	13.0
23	28.0
24	70.0
25	140.0
26	293.0
27	554.0
28	846.0
29	1392.0
30	1921.0
31	2638.0
32	3706.0
33	5837.0
34	12177.0
35	55365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47753536209372	20.01812233754619	13.73178940431641	25.77255289604368
2	14.50491263164088	22.378066717656058	37.91374948520327	25.203271165499796
3	17.182830113195113	26.950321229378954	29.38130986280093	26.485538794624997
4	11.445414538865224	18.71484715129551	36.731932318264185	33.107805991575084
5	13.030381474595815	38.367495822841406	33.86910785305815	14.733014849504626
6	30.24968818393618	38.49928223472101	18.301837949779966	12.949191631562845
7	28.176737071247864	31.935047361299052	21.742660469494616	18.145555097958464
8	27.512766808650834	33.21488245122726	20.213917586426	19.0584331536959
9	27.94963817144202	14.622580455374479	20.012943460610696	37.41483791257281
10	14.957757748335021	29.677829289530038	33.253712376156074	22.11070058597887
11	34.51627326853835	23.16734520979926	23.087332031158073	19.22904949050432
12	22.97201892076342	27.333913821099948	28.28701197844351	21.407055279693125
13	28.471748287953307	21.98126750288283	26.191372696679455	23.35561151248441
14	22.617842938836986	20.8740263102158	27.490410185206976	29.01772056574024
15	24.132777934670056	29.04076061376259	23.074931751859175	23.75152969970818
16	23.17911185371708	29.434259760431132	24.569929164803614	22.81669922104817
17	22.228367025157084	28.761207728331723	25.565387240251336	23.445038006259857
18	22.320146847716092	28.388205116136778	26.66674511095945	22.62490292518768
19	24.726425528910646	26.86207139999529	26.414938931118066	21.996564139975998
20	25.801023710066485	25.560981349649936	26.238748014355473	22.399246925928104
21	26.38434565693173	26.057232956016286	25.272397806697573	22.28602358035441
22	25.13472807285906	26.31962911538371	25.903089920692818	22.642552891064412
23	24.454014873387933	26.466158335686718	25.443612915372306	23.636213875553043
24	23.765090720824606	26.791471536488366	26.039582990139554	23.403854752547478
25	22.67367975148263	25.997834886566885	27.509884213499014	23.818601148451474
26	23.560560595897904	26.815405796589825	26.68949529894918	22.93453830856309
27	23.22004377809683	26.89763927789677	26.49045590415892	23.391861039847484
28	22.719463403153682	26.107319369263355	27.591197928924455	23.58201929865851
29	23.56796573472654	26.550880165678247	26.323778593617618	23.557375505977596
30	23.386404019722058	26.643602687659595	26.084654216824937	23.885339075793414
31	24.094471510273248	25.657228929840663	26.410365035656287	23.8379345242298
32	22.29883975430091	27.071615165564474	26.552681744369394	24.076863335765218
33	23.10489280082841	27.377562307312136	26.22673036643054	23.290814525428914
34	22.98631458796672	26.73068097574752	26.790694390510822	23.49231004577494
35	23.352905875292706	26.919501547368295	26.857136133108973	22.870456444230022
36	23.889150388326666	25.737820663685575	26.039068016003764	24.333960931984
37	24.093053904898625	25.795747384771072	26.69475071484885	23.416447995481448
38	23.40723481371649	26.551578055496716	26.013791804937746	24.027395325849042
39	22.799213941939964	26.336475212106237	26.7765735870371	24.0877372589167
40	22.770436440229222	26.375863407975714	26.52295164915336	24.330748502641704
41	23.358789404939813	26.495887412776675	26.521774943223942	23.623548239059577
42	23.591777178965202	25.58276361154584	26.592377298989213	24.233081910499745
43	23.812717688035395	25.557752047444225	26.93212840064012	23.69740186388026
44	22.09853735452973	26.844192367885345	27.43960556817246	23.61766470941247
45	23.019898097266513	25.833401974512547	26.74299565795512	24.40370427026582
46	22.473906546015083	25.911064565854346	27.194850734852853	24.420178153277714
47	22.07290955731802	26.751547386505376	27.229295615542114	23.946247440634487
48	22.4082746934645	25.62778000047069	27.069261726012567	24.894683580052245
49	22.640732373152595	25.391838463710815	28.25590699425774	23.711522168878847
50	22.497175939000282	25.454203144121244	28.088816718441116	23.959804198437354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	2.0
7	1.0
8	0.0
9	4.5
10	9.0
11	11.0
12	13.0
13	30.5
14	48.0
15	65.0
16	82.0
17	77.0
18	72.0
19	75.5
20	79.0
21	104.0
22	129.0
23	204.0
24	279.0
25	438.0
26	597.0
27	831.5
28	1066.0
29	1311.0
30	1556.0
31	1877.0
32	2198.0
33	2528.0
34	2858.0
35	3333.0
36	3808.0
37	4027.0
38	4246.0
39	4674.5
40	5103.0
41	5682.5
42	6262.0
43	5990.5
44	5719.0
45	6108.0
46	6497.0
47	6885.5
48	7274.0
49	7230.0
50	7186.0
51	7147.5
52	7109.0
53	6781.0
54	6453.0
55	5763.5
56	5074.0
57	4345.0
58	3616.0
59	2982.5
60	2349.0
61	2220.5
62	2092.0
63	1685.0
64	1278.0
65	927.0
66	576.0
67	510.0
68	444.0
69	347.5
70	251.0
71	222.5
72	194.0
73	183.5
74	173.0
75	162.0
76	151.0
77	107.0
78	63.0
79	47.0
80	31.0
81	24.5
82	18.0
83	14.0
84	10.0
85	7.5
86	5.0
87	6.0
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009413315134257407
2	0.001176664391782176
3	0.0
4	0.0
5	0.0
6	0.0
7	0.001176664391782176
8	0.0
9	0.001176664391782176
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.002353328783564352
16	0.0
17	0.0
18	0.0
19	0.0
20	0.001176664391782176
21	0.0
22	0.0
23	0.002353328783564352
24	0.0
25	0.002353328783564352
26	0.00588332195891088
27	0.014119972701386112
28	0.007059986350693056
29	0.002353328783564352
30	0.00588332195891088
31	0.009413315134257407
32	0.004706657567128704
33	0.004706657567128704
34	0.00588332195891088
35	0.003529993175346528
36	0.007059986350693056
37	0.003529993175346528
38	0.009413315134257407
39	0.00588332195891088
40	0.003529993175346528
41	0.003529993175346528
42	0.003529993175346528
43	0.002353328783564352
44	0.003529993175346528
45	0.003529993175346528
46	0.003529993175346528
47	0.004706657567128704
48	0.004706657567128704
49	0.002353328783564352
50	0.002353328783564352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	84986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.2027628079919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.047617580928325	23.703904172451935
2	13.46906089259864	10.29110677052691
3	6.831552037453415	7.829524862918598
4	4.244309606677549	6.485774127503354
5	2.861366926417593	5.465606099828206
6	2.1190747528259464	4.8572706092768225
7	1.5677457110296606	4.192455227919893
8	1.1642590938491393	3.5582331207493
9	0.9609757600024641	3.3040736121243497
>10	4.693996981550497	29.340126609088557
>50	0.040040656666769335	0.9719247876120773
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	97	0.11413644600287107	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	86	0.10119313769326713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.001176664391782176	0.0
18	0.0	0.0	0.0	0.001176664391782176	0.0
19	0.0	0.0	0.0	0.001176664391782176	0.0
20	0.0	0.0	0.0	0.001176664391782176	0.0
21	0.0	0.0	0.0	0.001176664391782176	0.0
22	0.0	0.0	0.0	0.001176664391782176	0.0
23	0.0	0.0	0.0	0.001176664391782176	0.0
24	0.0	0.0	0.0	0.002353328783564352	0.0
25	0.0	0.0	0.0	0.002353328783564352	0.0
26	0.0	0.0	0.0	0.002353328783564352	0.0
27	0.0	0.0	0.0	0.002353328783564352	0.0
28	0.0	0.0	0.0	0.0035299931753465276	0.0
29	0.0	0.0	0.0	0.011766643917821758	0.0
30	0.0	0.0	0.0	0.024709952227425693	0.0
31	0.0	0.0	0.0	0.08236650742475231	0.0
32	0.0	0.0	0.0	0.15296637093168286	0.0
33	0.0	0.0	0.0	0.23180288518108866	0.0
34	0.0	0.0	0.0	0.31299272821405877	0.0
35	0.0	0.0	0.0	0.41771585908267245	0.0
36	0.0	0.0	0.0	0.5647989080554444	0.0
37	0.0	0.0	0.0	0.8013084508036618	0.0
38	0.0	0.0	0.0	1.1095945214505918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTTAC	20	7.793192E-4	44.025326	1
GTAGACT	20	7.8158465E-4	43.999413	4
GGTATCA	75	1.8189894E-12	35.22026	1
TAGGTCC	25	0.0023406933	35.19953	4
GTTTGGG	35	3.193187E-4	31.42815	20
GCCCAGA	30	0.005699314	29.350216	1
TGTTTGG	30	0.005715755	29.332941	19
TGCATCT	30	0.005715755	29.332941	30
GTATCAA	200	0.0	28.61646	1
AAACCAC	45	0.0013876625	24.444117	19
GTTTCGT	50	0.002561122	21.999706	11
TATAGTA	50	0.002561122	21.999706	2
GAATTTT	50	0.002561122	21.999706	20
TATCAAC	270	0.0	20.370098	2
CTTCTAG	65	4.8615207E-4	20.31938	1
GGAATTT	55	0.0044503217	19.999733	19
ATAGTAG	55	0.0044503217	19.999733	3
CCCTACA	60	0.0073575126	18.333088	2
ATGGAAT	60	0.0073575126	18.333088	17
AAAGCTC	60	0.0073575126	18.333088	40
>>END_MODULE