FastQCFastQC Report
Thu 2 Feb 2017
SRR4063911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868356
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48530.2597470717572026No Hit
TATCAACGCAGAGTACTTTTTTTTT33970.1818175979310153No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21980.11764353260299429No Hit
GTCCTAAAGTGTGTATTTCTCATTT20620.11036440592692184No Hit
GTACATGGGGTGGTATCAACGCAAA18800.10062322169864844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT954.9003575E-914.00067113
TTAGACC551.9654841E-413.81403353
GTCCAAT655.4731405E-513.1488421
CGCGCTA905.400252E-712.66795316
CCCTTAT1600.012.4640061
GCGAAAT1001.4411125E-712.35125312
CGGACAT1551.8189894E-1212.257015
CGGTGAA550.00306528312.09278317
ACCGTAT550.003067128412.0918128
TAATACC802.8711822E-511.8733434
GCGACGG658.0215244E-411.69286711
GTTCTAG1157.11334E-811.5608181
TAGAAAT3900.011.4471214
GTATAAA1850.011.2929991
CTAGGAC2200.011.2239023
GTCCTAA6300.011.1544851
GAGTTAG600.005893075411.0791171
GTATCAA35750.010.890961
CGCCCGA1053.4626137E-610.85824516
TAGACCT1053.4726272E-610.8556284
Produced by FastQC (version 0.11.5)