##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063911_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868356
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19499335244461	32.0	32.0	32.0	32.0	32.0
2	30.681569251256185	32.0	32.0	32.0	32.0	32.0
3	30.708981050720528	32.0	32.0	32.0	32.0	32.0
4	30.741339980175084	32.0	32.0	32.0	32.0	32.0
5	30.587966104960724	32.0	32.0	32.0	32.0	32.0
6	34.28117500090989	36.0	36.0	36.0	32.0	36.0
7	34.2082290527073	36.0	36.0	36.0	32.0	36.0
8	34.15078657386494	36.0	36.0	36.0	32.0	36.0
9	34.34539563123944	36.0	36.0	36.0	32.0	36.0
10	33.933789384892385	36.0	36.0	36.0	32.0	36.0
11	34.345939424820536	36.0	36.0	36.0	32.0	36.0
12	34.0842885402996	36.0	36.0	36.0	32.0	36.0
13	34.211926420874825	36.0	36.0	36.0	32.0	36.0
14	34.08069821811261	36.0	36.0	36.0	32.0	36.0
15	33.99758450744933	36.0	36.0	36.0	32.0	36.0
16	34.00004602977163	36.0	36.0	36.0	32.0	36.0
17	33.92223055991471	36.0	36.0	36.0	32.0	36.0
18	33.918917486817286	36.0	36.0	36.0	32.0	36.0
19	33.92606976400643	36.0	36.0	36.0	32.0	36.0
20	33.90320420733522	36.0	36.0	36.0	32.0	36.0
21	33.8852418918022	36.0	36.0	36.0	32.0	36.0
22	33.861826654020966	36.0	36.0	36.0	32.0	36.0
23	33.81034021353532	36.0	36.0	36.0	32.0	36.0
24	33.782814410101714	36.0	36.0	36.0	32.0	36.0
25	33.36114958819411	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	14.0
5	20.0
6	75.0
7	41.0
8	122.0
9	122.0
10	158.0
11	66.0
12	125.0
13	97.0
14	180.0
15	291.0
16	540.0
17	747.0
18	988.0
19	1490.0
20	2285.0
21	3408.0
22	5426.0
23	8434.0
24	12367.0
25	17776.0
26	24448.0
27	31675.0
28	42475.0
29	56734.0
30	73900.0
31	98896.0
32	137834.0
33	190767.0
34	390111.0
35	766738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22690071818563	17.388627147984362	11.505748215780166	25.87872391804984
2	16.41730728262859	19.75983993611147	38.54748872476655	25.275364056493387
3	18.893658473923097	24.311895178556135	28.466483257686047	28.327963089834718
4	12.452810596332755	15.9196025839962	36.13408989818918	35.493496921481864
5	14.545899315696165	36.84845539178321	33.51525766308956	15.09038762943106
6	33.3936802729209	35.902182285942786	17.384736363991614	13.319401077144693
7	30.149178620853956	30.4247829776299	20.802186418401465	18.623851983114683
8	28.04010785665944	33.4570886412117	19.211164320777186	19.291639181351677
9	27.95525146285262	14.164890144815315	18.492052909559913	39.38780548277215
10	16.436844890116127	26.658594163376613	31.329595917833984	25.574965028673276
11	36.77599292246197	20.673951275520587	23.33622161433844	19.213834187678998
12	25.47452354836498	23.40011654212655	29.167559299376155	21.95780061013231
13	29.948444718663268	19.750825066599827	25.221276381511228	25.079453833225678
14	23.706277376015905	20.317462697918753	25.0745827383675	30.90167718769784
15	25.07313081284189	28.197431236684622	22.107302798518855	24.622135151954634
16	25.30159522503685	25.64159282555788	24.628348558598702	24.428463390806566
17	23.633642945779528	26.06287687321957	25.527575645106264	24.77590453589464
18	24.302953168823212	24.982751934247375	27.20057464984284	23.513720247086578
19	26.187248351985037	24.400852088146237	25.125298415645254	24.28660114422347
20	25.931510664966844	24.55197497402055	25.222191273046718	24.29432308796589
21	26.988202072055955	23.635600110983866	24.3059490995416	25.070248717418576
22	25.97946184704106	24.66560285226532	24.805141448212435	24.549793852481187
23	24.336550787257533	24.690633207574255	25.617622055317206	25.355193949851003
24	25.121417533297084	25.025804741001817	25.329194576049662	24.52358314965143
25	24.80524774500913	24.862402570306596	25.51349619869524	24.818853485989035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	156.0
2	452.0
3	748.0
4	748.0
5	748.0
6	1881.5
7	3015.0
8	3015.0
9	3015.0
10	2973.0
11	2931.0
12	2931.0
13	2931.0
14	3285.0
15	3639.0
16	3639.0
17	3639.0
18	6662.5
19	9686.0
20	9686.0
21	9686.0
22	17448.5
23	25211.0
24	25211.0
25	25211.0
26	40824.0
27	56437.0
28	56437.0
29	56437.0
30	73587.0
31	90737.0
32	90737.0
33	90737.0
34	108046.5
35	125356.0
36	125356.0
37	125356.0
38	140644.0
39	155932.0
40	155932.0
41	155932.0
42	179317.5
43	202703.0
44	202703.0
45	202703.0
46	222731.5
47	242760.0
48	242760.0
49	242760.0
50	248510.0
51	254260.0
52	254260.0
53	254260.0
54	233179.0
55	212098.0
56	212098.0
57	212098.0
58	193662.5
59	175227.0
60	175227.0
61	175227.0
62	154563.5
63	133900.0
64	133900.0
65	133900.0
66	109674.0
67	85448.0
68	85448.0
69	85448.0
70	63942.5
71	42437.0
72	42437.0
73	42437.0
74	32267.0
75	22097.0
76	22097.0
77	22097.0
78	17622.5
79	13148.0
80	13148.0
81	13148.0
82	9120.5
83	5093.0
84	5093.0
85	5093.0
86	3894.0
87	2695.0
88	2695.0
89	2695.0
90	1935.0
91	1175.0
92	1175.0
93	1175.0
94	831.0
95	487.0
96	487.0
97	487.0
98	733.5
99	980.0
100	980.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03923235186441984
2	0.00545934500705433
3	0.0019268276495485873
4	0.004656500153075752
5	0.009634138247742936
6	0.014397684381349167
7	0.02467409851227496
8	0.03703790926354506
9	0.050472179820119936
10	0.05951756517494525
11	0.05566390987584807
12	0.06529804812359101
13	0.06604736998730434
14	0.06968693332534057
15	0.06176553076608527
16	0.06872351950056628
17	0.06331769748377719
18	0.07921402559255303
19	0.07562798524478205
20	0.08007039343679685
21	0.07589560019610823
22	0.07969573250494018
23	0.08344234182350686
24	0.0776618588748611
25	0.08012391642706208
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1868356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.565720789947996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.30377429975648	30.628346016227127
2	15.232169493058434	13.272008863194952
3	5.961220807533969	7.791146438047597
4	2.824901658609169	4.922755076721122
5	1.5202832251519947	3.311611725430672
6	0.9608471188872364	2.5115998381960387
7	0.6167871445181528	1.8809543567435003
8	0.43835056851166704	1.5277646780715408
9	0.31039888550730554	1.217047606157008
>10	1.5773549756721394	12.40779121477567
>50	0.12752865665686364	3.8537626600166712
>100	0.10910489636617506	9.801940061181195
>500	0.01246503747731337	3.643961911647194
>1k	0.004813232293220015	3.229309553589809
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4853	0.2597470717572026	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3397	0.1818175979310153	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2198	0.11764353260299429	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2062	0.11036440592692184	No Hit
GTACATGGGGTGGTATCAACGCAAA	1880	0.10062322169864844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.352299026523853E-5
2	0.0	0.0	0.0	0.0	5.352299026523853E-5
3	0.0	0.0	0.0	0.0	5.352299026523853E-5
4	0.0	0.0	0.0	0.0	5.352299026523853E-5
5	0.0	0.0	0.0	0.0	5.352299026523853E-5
6	0.0	0.0	0.0	0.0	1.6056897079571558E-4
7	0.0	0.0	0.0	0.0	1.6056897079571558E-4
8	0.0	0.0	0.0	0.0	1.6056897079571558E-4
9	0.0	0.0	0.0	5.352299026523853E-5	1.6056897079571558E-4
10	0.0	0.0	0.0	5.352299026523853E-5	1.6056897079571558E-4
11	0.0	0.0	0.0	1.0704598053047706E-4	1.6056897079571558E-4
12	0.0	0.0	0.0	1.6056897079571558E-4	3.2113794159143117E-4
13	0.0	0.0	0.0	1.6056897079571558E-4	4.2818392212190824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	95	4.9003575E-9	14.000671	13
TTAGACC	55	1.9654841E-4	13.8140335	3
GTCCAAT	65	5.4731405E-5	13.148842	1
CGCGCTA	90	5.400252E-7	12.667953	16
CCCTTAT	160	0.0	12.464006	1
GCGAAAT	100	1.4411125E-7	12.351253	12
CGGACAT	155	1.8189894E-12	12.25701	5
CGGTGAA	55	0.003065283	12.092783	17
ACCGTAT	55	0.0030671284	12.091812	8
TAATACC	80	2.8711822E-5	11.873343	4
GCGACGG	65	8.0215244E-4	11.692867	11
GTTCTAG	115	7.11334E-8	11.560818	1
TAGAAAT	390	0.0	11.447121	4
GTATAAA	185	0.0	11.292999	1
CTAGGAC	220	0.0	11.223902	3
GTCCTAA	630	0.0	11.154485	1
GAGTTAG	60	0.0058930754	11.079117	1
GTATCAA	3575	0.0	10.89096	1
CGCCCGA	105	3.4626137E-6	10.858245	16
TAGACCT	105	3.4726272E-6	10.855628	4
>>END_MODULE