##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868356
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255632759495512	32.0	32.0	32.0	32.0	32.0
2	31.380312424398777	32.0	32.0	32.0	32.0	32.0
3	31.474612975257394	32.0	32.0	32.0	32.0	32.0
4	31.56249290820379	32.0	32.0	32.0	32.0	32.0
5	31.517520750863326	32.0	32.0	32.0	32.0	32.0
6	35.09210129118862	36.0	36.0	36.0	36.0	36.0
7	35.12079924810903	36.0	36.0	36.0	36.0	36.0
8	35.06804110137468	36.0	36.0	36.0	36.0	36.0
9	35.18042439449441	36.0	36.0	36.0	36.0	36.0
10	35.027687442864206	36.0	36.0	36.0	36.0	36.0
11	35.18022689466033	36.0	36.0	36.0	36.0	36.0
12	35.090898629597355	36.0	36.0	36.0	36.0	36.0
13	35.12758114620554	36.0	36.0	36.0	36.0	36.0
14	35.06543506697867	36.0	36.0	36.0	36.0	36.0
15	35.03931798864885	36.0	36.0	36.0	36.0	36.0
16	35.05326500945216	36.0	36.0	36.0	36.0	36.0
17	35.02072998935963	36.0	36.0	36.0	36.0	36.0
18	35.009717634112555	36.0	36.0	36.0	36.0	36.0
19	35.011902442575185	36.0	36.0	36.0	36.0	36.0
20	35.0041571306539	36.0	36.0	36.0	36.0	36.0
21	34.99484038373843	36.0	36.0	36.0	36.0	36.0
22	34.9727974754276	36.0	36.0	36.0	36.0	36.0
23	34.927916307170584	36.0	36.0	36.0	36.0	36.0
24	34.90646643359189	36.0	36.0	36.0	32.0	36.0
25	34.86699269304137	36.0	36.0	36.0	32.0	36.0
26	34.81990851850504	36.0	36.0	36.0	32.0	36.0
27	34.79714144413592	36.0	36.0	36.0	32.0	36.0
28	34.76726169959044	36.0	36.0	36.0	32.0	36.0
29	34.73864991468435	36.0	36.0	36.0	32.0	36.0
30	34.71793330607229	36.0	36.0	36.0	32.0	36.0
31	34.71050377979357	36.0	36.0	36.0	32.0	36.0
32	34.65683681268452	36.0	36.0	36.0	32.0	36.0
33	34.629363997011275	36.0	36.0	36.0	32.0	36.0
34	34.6090889530689	36.0	36.0	36.0	32.0	36.0
35	34.576116650145906	36.0	36.0	36.0	32.0	36.0
36	34.54409652121972	36.0	36.0	36.0	32.0	36.0
37	34.52077334298174	36.0	36.0	36.0	32.0	36.0
38	34.48236578039731	36.0	36.0	36.0	32.0	36.0
39	34.46937307451042	36.0	36.0	36.0	32.0	36.0
40	34.460280588924164	36.0	36.0	36.0	32.0	36.0
41	34.45087927568408	36.0	36.0	36.0	32.0	36.0
42	34.38489666851499	36.0	36.0	36.0	32.0	36.0
43	34.377137975846146	36.0	36.0	36.0	32.0	36.0
44	34.299849707443336	36.0	36.0	36.0	32.0	36.0
45	34.270434007223464	36.0	36.0	36.0	32.0	36.0
46	34.21791778440512	36.0	36.0	36.0	32.0	36.0
47	34.18887085758817	36.0	36.0	36.0	32.0	36.0
48	34.13094024907459	36.0	36.0	36.0	32.0	36.0
49	34.107282016917544	36.0	36.0	36.0	32.0	36.0
50	33.52501129335094	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	22.0
21	76.0
22	217.0
23	686.0
24	1618.0
25	3868.0
26	7559.0
27	13889.0
28	22491.0
29	33484.0
30	47880.0
31	67042.0
32	97178.0
33	156251.0
34	325337.0
35	1090751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.224726913798065	18.28370132904539	12.051763665550967	26.439808091605588
2	15.61471182815612	20.503578831983564	38.06787497183339	25.81383436802692
3	18.09255123106015	24.81062157016703	28.864949749752324	28.231877449020498
4	12.083510851251047	16.567934590624056	36.28810569291934	35.060448865205565
5	14.156723879175063	37.24054730468926	33.23386977642377	15.368859039711918
6	33.430531291748025	36.25772873634091	17.08098902134073	13.230750950570341
7	29.995418422224507	30.575501832631108	20.830121947042098	18.598957798102283
8	27.838377696755867	33.710331435764914	19.104549668264507	19.346741199214712
9	27.84548809501508	13.953332780256217	18.431758758466763	39.76942036626194
10	16.28184178567166	26.871301193198878	31.313321618828276	25.53353540230119
11	37.0015136301647	20.600945430100044	23.217416809216232	19.180124130519022
12	25.288783603107333	23.429767592222888	29.294876666841507	21.98657213782827
13	29.935274128223917	19.737831584277174	25.25636380082651	25.0705304866724
14	23.63896096667009	20.238852207721028	25.116519799266946	31.005667026341932
15	25.03348002813286	28.263031518863897	22.09232244880045	24.6111660042028
16	25.406870786712304	25.659661358046826	24.55879401288306	24.374673842357808
17	23.660158984690284	26.2079068443059	25.441297054736893	24.690637116266924
18	24.319907940161105	24.943773918163085	27.209355848743545	23.526962292932264
19	26.218718488339483	24.301096793116514	25.22303030043525	24.257154418108755
20	26.03618609440884	24.42443621135745	25.2656633528551	24.273714341378614
21	26.96209930013338	23.594165137693246	24.422754549989403	25.020981012183974
22	25.89837145336026	24.576083855673954	24.973706788813463	24.551837902152325
23	24.35010525380538	24.59422557231255	25.761303744873125	25.29436542900895
24	25.26370890219595	24.907190737485735	25.33751742180793	24.491582938510383
25	24.821832199250345	24.777728476285954	25.599106792562097	24.801332531901604
26	24.34479141018916	25.55934398852408	26.073833406485182	24.02203119480158
27	25.12842362641451	25.004884299639606	25.123980920988892	24.742711152956996
28	24.166116694687137	25.066732609778956	25.699187004924855	25.067963690609048
29	24.61045050291386	24.597979352485027	25.312635148733186	25.47893499586793
30	24.024411719209564	25.170597816283724	26.08063125340219	24.724359211104527
31	25.4426926934964	24.497839126529662	25.00125787779423	25.058210302179717
32	24.756033331049604	25.179790267881614	24.808006046177034	25.25617035489175
33	24.193226371920623	24.91571101684709	25.668865664851264	25.222196946381022
34	24.974321507042518	24.913142731742045	25.78993146157325	24.322604299642187
35	25.31947475272989	24.943946398358722	25.570826114207506	24.165752734703883
36	24.22493068268191	25.378220980398453	25.420935435869435	24.975912901050197
37	25.230987613218993	25.08898549576563	24.809637945449346	24.87038894556603
38	24.755315350807	25.3619352314314	25.197554250808658	24.685195166952948
39	25.587706339520842	24.60883807566318	24.820369537756655	24.983086047059327
40	25.85666763902233	24.905810850953934	25.26094368454998	23.976577825473754
41	24.8582532648717	24.897700954398577	25.90332265159126	24.34072312913846
42	25.664191898295957	25.735806924177457	25.064670295232677	23.535330882293906
43	24.610934720102083	24.92715396258893	25.554240056863797	24.90767126044519
44	24.270603738319757	25.544043170196208	24.915030888613657	25.270322202870375
45	24.53240402005424	25.601913577504654	25.04660571750325	24.81907668493786
46	24.66793981382364	24.93678867184489	25.07043700918892	25.324834505142547
47	25.26746010846131	24.92806356098219	25.163997953237033	24.640478377319468
48	25.283329711537327	25.469968174582704	24.356292665381375	24.890409448498595
49	24.895349005246867	25.517394196921035	24.699775684787618	24.887481113044483
50	24.723430516015043	26.082981817520075	24.29465348060869	24.89893418585619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	31.5
2	26.0
3	87.0
4	148.0
5	178.5
6	209.0
7	205.0
8	201.0
9	301.5
10	402.0
11	611.0
12	820.0
13	1573.0
14	2326.0
15	3297.5
16	4269.0
17	4866.0
18	5463.0
19	5587.0
20	5711.0
21	6161.5
22	6612.0
23	7293.5
24	7975.0
25	8871.0
26	9767.0
27	12834.0
28	15901.0
29	19937.5
30	23974.0
31	29196.0
32	34418.0
33	41854.0
34	49290.0
35	57807.0
36	66324.0
37	73038.0
38	79752.0
39	82400.0
40	85048.0
41	90314.5
42	95581.0
43	96599.0
44	97617.0
45	106329.5
46	115042.0
47	122584.5
48	130127.0
49	135513.5
50	140900.0
51	138399.5
52	135899.0
53	134227.0
54	132555.0
55	132261.0
56	131967.0
57	128907.5
58	125848.0
59	114889.0
60	103930.0
61	91250.0
62	78570.0
63	67332.5
64	56095.0
65	48145.5
66	40196.0
67	34150.5
68	28105.0
69	25162.5
70	22220.0
71	17410.0
72	12600.0
73	10609.5
74	8619.0
75	6658.5
76	4698.0
77	4143.0
78	3588.0
79	3003.0
80	2418.0
81	1891.5
82	1365.0
83	1141.5
84	918.0
85	662.0
86	406.0
87	276.5
88	147.0
89	107.5
90	68.0
91	69.0
92	70.0
93	58.0
94	46.0
95	46.0
96	46.0
97	32.5
98	19.0
99	21.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009259477315886265
2	4.8170691238714683E-4
3	3.746609318566697E-4
4	0.0
5	0.0
6	2.1409196106095412E-4
7	2.1409196106095412E-4
8	0.0
9	0.0011239827955700091
10	9.098908345090551E-4
11	0.0
12	1.0704598053047706E-4
13	8.02844853978578E-4
14	2.1409196106095412E-4
15	0.004174793240688606
16	5.887528929176239E-4
17	0.0
18	3.2113794159143117E-4
19	0.0
20	7.493218637133394E-4
21	0.0
22	1.6056897079571558E-4
23	8.02844853978578E-4
24	4.2818392212190824E-4
25	0.0017662586787528713
26	0.005138207065462899
27	0.006690373783154816
28	0.004228316230953844
29	0.0023550115716704954
30	0.003907178289362413
31	0.006957988734481009
32	0.0036395633380362203
33	0.004335362211484321
34	0.00326490240617955
35	0.002943764464588119
36	0.0072791266760724406
37	0.003960701279627651
38	0.007225603685807202
39	0.004067747260158129
40	0.0021944426008747796
41	0.0031578564256490734
42	0.0017662586787528713
43	0.001712735688487633
44	0.001605689707957156
45	0.001231028776100486
46	0.0010704598053047705
47	0.002140919610609541
48	0.0020338736300790643
49	1.6056897079571558E-4
50	0.0010169368150395321
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1868356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.3055777416611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08818560543511	33.62867690671544
2	15.188254029740145	14.369782635279396
3	5.829656851444252	8.2732585617961
4	2.647090723090465	5.008886239615435
5	1.4589915666165194	3.4509219489502843
6	0.8887757332298657	2.5226429725924353
7	0.5982519246293484	1.9810457050756414
8	0.4045330540944209	1.530933587162818
9	0.28381923745949583	1.2083609701997238
>10	1.4113735456076364	11.73741542758194
>50	0.10373796351358978	3.4218984152680725
>100	0.08549166504925874	8.222793551254343
>500	0.009333886609391504	3.088929647566919
>1k	0.0025042134805684526	1.5544534309415465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.352299026523853E-5
2	0.0	0.0	0.0	0.0	5.352299026523853E-5
3	0.0	0.0	0.0	0.0	5.352299026523853E-5
4	0.0	0.0	0.0	0.0	1.0704598053047706E-4
5	0.0	0.0	0.0	0.0	1.0704598053047706E-4
6	0.0	0.0	0.0	0.0	1.6056897079571558E-4
7	0.0	0.0	0.0	0.0	1.6056897079571558E-4
8	0.0	0.0	0.0	0.0	1.6056897079571558E-4
9	0.0	0.0	0.0	0.0	1.6056897079571558E-4
10	0.0	0.0	0.0	0.0	1.6056897079571558E-4
11	0.0	0.0	0.0	5.352299026523853E-5	1.6056897079571558E-4
12	0.0	0.0	0.0	1.0704598053047706E-4	3.2113794159143117E-4
13	0.0	0.0	0.0	1.6056897079571558E-4	4.2818392212190824E-4
14	0.0	0.0	0.0	1.6056897079571558E-4	4.817069123871468E-4
15	0.0	0.0	0.0	2.6761495132619263E-4	6.422758831828623E-4
16	0.0	0.0	0.0	3.2113794159143117E-4	6.422758831828623E-4
17	0.0	0.0	0.0	3.746609318566697E-4	6.422758831828623E-4
18	0.0	0.0	0.0	3.746609318566697E-4	8.02844853978578E-4
19	0.0	0.0	0.0	5.352299026523853E-4	8.02844853978578E-4
20	0.0	0.0	0.0	8.02844853978578E-4	8.02844853978578E-4
21	0.0	0.0	0.0	0.0010704598053047705	8.02844853978578E-4
22	0.0	0.0	0.0	0.001605689707957156	8.02844853978578E-4
23	0.0	0.0	0.0	0.0028902414743228804	0.0010169368150395321
24	0.0	0.0	0.0	0.004388885201749559	0.0010704598053047705
25	0.0	0.0	0.0	0.005245253045993376	0.0010704598053047705
26	0.0	0.0	0.0	0.006155143880502431	0.0011239827955700091
27	0.0	0.0	0.0	0.006850942753950532	0.0011775057858352477
28	0.0	0.0	0.0	0.009045385354825311	0.0011775057858352477
29	0.0	0.0	0.0	0.01273847168312677	0.0011775057858352477
30	0.0	0.0	0.0	0.019000661544159678	0.0011775057858352477
31	0.0	0.0	0.0	0.035539265536118386	0.0011775057858352477
32	0.0	0.0	0.0	0.05325537531391234	0.0011775057858352477
33	0.0	0.0	0.0	0.06808124361738341	0.0011775057858352477
34	0.0	0.0	0.0	0.08606496834650355	0.0012310287761004863
35	0.0	0.0	0.0	0.10581495175437658	0.0012310287761004863
36	0.0	0.0	0.0	0.13455679752680966	0.0012310287761004863
37	0.0	0.0	0.0	0.17812451160271384	0.0012310287761004863
38	0.0	0.0	0.0	0.24063936423251242	0.0012310287761004863
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGC	30	0.0057461634	29.33296	6
GTATCAA	4110	0.0	19.96782	1
TGTAGGA	1560	0.0	19.884361	2
TTGTCGG	135	2.6011548E-9	19.554783	22
CTGTAGG	1585	0.0	18.878752	1
GTAGGAC	1550	0.0	18.593311	3
TCGCATT	60	0.0074152676	18.33261	18
CGGTACG	85	1.4312087E-4	18.1179	38
GTCCTAG	390	0.0	18.052984	1
TACCGTC	485	0.0	17.690496	7
GTCCTAA	955	0.0	17.279116	1
AACGCAG	4915	0.0	17.008938	6
TAGGACG	1295	0.0	16.9882	4
GTCCTAC	1360	0.0	16.339775	1
CCGTCGT	490	0.0	16.163492	9
ACCGTCG	490	0.0	16.16306	8
TCCTACA	1430	0.0	16.153639	2
CGTCGTA	525	0.0	15.923607	10
CGAAATT	180	5.2714313E-9	15.888685	13
GCGAAAT	180	5.2714313E-9	15.888685	12
>>END_MODULE