FastQCFastQC Report
Thu 2 Feb 2017
SRR4063910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766491
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37690.13623756592737876No Hit
GTCCTAAAGTGTGTATTTCTCATTT32590.11780266048217759No Hit
GTCCTACAGTGGACATTTCTAAATT28240.10207877054362367No Hit
CTTTAGGACGTGAAATATGGCGAGG27830.1005967487333232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA250.0060322819.0005259
GGTATCA10350.014.9566241
GTATCAA36150.013.73977851
CGGGTTC3200.013.65885117
TCGCGCG1403.6379788E-1212.8932139
TCCAACG2300.012.807713518
AGCCGAC3200.012.7666711
GATCGCT2150.011.9312111
ATCGCTT2100.011.76308212
GACGGGT3600.011.613111515
CGTCGTA3600.011.61185110
GTCTTAC1501.7826096E-1011.3963981
AACGATC2300.011.1524828
TAGGTCG1803.6379788E-1211.0818355
ACCGTAA951.3607294E-511.0003038
GTCCTAT5050.010.9074431
TTAGAAC1408.401912E-910.8537123
GCGTTAT1752.0008883E-1110.8537121
CGTGTCG1158.820916E-710.7394289
ACCGACA3900.010.7164995
Produced by FastQC (version 0.11.5)