##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063910_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2766491
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279181461280736	32.0	32.0	32.0	32.0	32.0
2	30.851266098461913	32.0	32.0	32.0	32.0	32.0
3	30.871320022367684	32.0	32.0	32.0	32.0	32.0
4	30.908248752661766	32.0	32.0	32.0	32.0	32.0
5	30.790839008693684	32.0	32.0	32.0	32.0	32.0
6	34.461497615571496	36.0	36.0	36.0	32.0	36.0
7	34.41468379980271	36.0	36.0	36.0	32.0	36.0
8	34.37892586673877	36.0	36.0	36.0	32.0	36.0
9	34.529094076214236	36.0	36.0	36.0	32.0	36.0
10	34.207173636205574	36.0	36.0	36.0	32.0	36.0
11	34.49886842212752	36.0	36.0	36.0	32.0	36.0
12	34.31367353083744	36.0	36.0	36.0	32.0	36.0
13	34.38372544859173	36.0	36.0	36.0	32.0	36.0
14	34.2927072598465	36.0	36.0	36.0	32.0	36.0
15	34.22514369285857	36.0	36.0	36.0	32.0	36.0
16	34.22652378048582	36.0	36.0	36.0	32.0	36.0
17	34.15530034256392	36.0	36.0	36.0	32.0	36.0
18	34.150652216110586	36.0	36.0	36.0	32.0	36.0
19	34.152791749548435	36.0	36.0	36.0	32.0	36.0
20	34.133673668195556	36.0	36.0	36.0	32.0	36.0
21	34.12391184355922	36.0	36.0	36.0	32.0	36.0
22	34.0971486984776	36.0	36.0	36.0	32.0	36.0
23	34.046441141503806	36.0	36.0	36.0	32.0	36.0
24	34.017469060987366	36.0	36.0	36.0	32.0	36.0
25	33.60929784336909	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	25.0
5	27.0
6	116.0
7	46.0
8	140.0
9	185.0
10	259.0
11	91.0
12	171.0
13	144.0
14	226.0
15	397.0
16	738.0
17	1023.0
18	1276.0
19	1808.0
20	2767.0
21	4001.0
22	6158.0
23	9455.0
24	13982.0
25	20852.0
26	29630.0
27	39833.0
28	54343.0
29	74149.0
30	98164.0
31	134618.0
32	190871.0
33	271407.0
34	577458.0
35	1232122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72973476281032	17.412520832677934	12.108138638872648	25.749605765639107
2	16.20720543559831	19.558401015632118	38.656521431081174	25.577872117688393
3	18.904003721735897	23.606490771027573	28.464303181223887	29.025202326012646
4	12.80226634465891	15.811347122408131	35.952050196772475	35.434336336160484
5	14.72758309299489	36.54598772773709	33.67363907227072	15.052790106997302
6	33.576957142598914	35.81982177394573	17.136163982430457	13.46705710102489
7	30.422078133372093	30.23739951682752	20.488190764189124	18.852331585611264
8	27.78468832738263	33.708612336729225	19.02510867843694	19.481590657451207
9	27.351164547614214	14.534142981703349	18.44698535450686	39.66770711617557
10	16.05543841120921	26.7415104507626	31.377967137695446	25.825084000332748
11	36.7217956354696	20.840182410593094	23.25387861210841	19.184143341828904
12	25.42859519736104	23.165083027999117	29.211243050858492	22.195078723781343
13	29.713081548442354	20.104076843390647	24.955293327854715	25.227548280312277
14	23.457135312862842	19.862549418909584	25.072143469469694	31.60817179875788
15	25.069081755182943	28.289702116578177	21.658359977430884	24.982856150808004
16	25.367150509825763	25.7812324798258	24.428014916789344	24.423602093559087
17	23.787290051031203	26.236708250460527	25.281912225172427	24.694089473335843
18	24.328870714279514	25.313497541507857	26.698226239158178	23.65940550505445
19	26.030035505056258	24.49932082788458	24.93341219326471	24.537231473794446
20	26.32369863989949	24.42269798186219	24.88173235644278	24.371871021795542
21	26.662979376375272	23.80186879781917	24.411393075876546	25.12375874992901
22	26.18562093773096	24.809364792890527	24.577661485990543	24.427352783387978
23	24.59402241233605	24.96888783413019	25.124629548164528	25.312460205369234
24	25.26620153070176	24.7609838540881	25.223876979531916	24.74893763567822
25	24.998552989523645	24.770467963188054	25.256554957457894	24.97442408983041
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	77.0
2	269.0
3	461.0
4	461.0
5	461.0
6	1112.0
7	1763.0
8	1763.0
9	1763.0
10	2207.0
11	2651.0
12	2651.0
13	2651.0
14	3939.0
15	5227.0
16	5227.0
17	5227.0
18	10056.0
19	14885.0
20	14885.0
21	14885.0
22	25551.5
23	36218.0
24	36218.0
25	36218.0
26	58602.5
27	80987.0
28	80987.0
29	80987.0
30	108311.5
31	135636.0
32	135636.0
33	135636.0
34	162208.0
35	188780.0
36	188780.0
37	188780.0
38	213715.0
39	238650.0
40	238650.0
41	238650.0
42	267836.0
43	297022.0
44	297022.0
45	297022.0
46	326824.5
47	356627.0
48	356627.0
49	356627.0
50	365260.5
51	373894.0
52	373894.0
53	373894.0
54	344904.0
55	315914.0
56	315914.0
57	315914.0
58	285783.5
59	255653.0
60	255653.0
61	255653.0
62	223556.5
63	191460.0
64	191460.0
65	191460.0
66	159538.0
67	127616.0
68	127616.0
69	127616.0
70	97278.0
71	66940.0
72	66940.0
73	66940.0
74	53984.5
75	41029.0
76	41029.0
77	41029.0
78	29916.5
79	18804.0
80	18804.0
81	18804.0
82	13259.5
83	7715.0
84	7715.0
85	7715.0
86	6288.0
87	4861.0
88	4861.0
89	4861.0
90	3188.5
91	1516.0
92	1516.0
93	1516.0
94	1121.0
95	726.0
96	726.0
97	726.0
98	1052.5
99	1379.0
100	1379.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03629146091565091
2	0.004879827911965013
3	0.0014458749368785222
4	0.0035423935953523794
5	0.009145158975756653
6	0.012326083836889403
7	0.02186885842028765
8	0.03589384530800931
9	0.0484368103854305
10	0.05859408181700212
11	0.054943247601383846
12	0.06365464409607693
13	0.06517281277979939
14	0.06726933143827324
15	0.05967848801966101
16	0.06582345650139472
17	0.06188344729840075
18	0.07934238716120891
19	0.07576384669243456
20	0.07988459026253836
21	0.07373962178080465
22	0.07880018405987947
23	0.08310166199709307
24	0.07728201537615702
25	0.0784748621990818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2766491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42337854309471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.51783370396707	30.621225869663643
2	15.334003071578858	13.317084399162916
3	5.806302512406888	7.5638781579589836
4	2.6708807695456733	4.639146667978155
5	1.4755972891078817	3.203770983104795
6	0.8899348160820848	2.3186385838447037
7	0.5928657703516674	1.8020964339856733
8	0.4186918959553749	1.4544813352797015
9	0.3014727682817629	1.17818695237803
>10	1.72150230008022	13.965359522416954
>50	0.15205398726303537	4.58829228438304
>100	0.10358217212065736	9.07419475513924
>500	0.01060341963861953	3.223054133732379
>1k	0.004675523620172812	3.050589920971771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3769	0.13623756592737876	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3259	0.11780266048217759	No Hit
GTCCTACAGTGGACATTTCTAAATT	2824	0.10207877054362367	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2783	0.1005967487333232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1688124053177835E-4
2	0.0	0.0	0.0	0.0	2.1688124053177835E-4
3	0.0	0.0	0.0	0.0	2.1688124053177835E-4
4	0.0	0.0	0.0	0.0	2.1688124053177835E-4
5	3.614687342196306E-5	0.0	0.0	0.0	2.530281139537414E-4
6	3.614687342196306E-5	0.0	0.0	0.0	2.891749873757045E-4
7	3.614687342196306E-5	0.0	0.0	0.0	2.891749873757045E-4
8	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	3.253218607976675E-4
9	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	3.6146873421963056E-4
10	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	3.6146873421963056E-4
11	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	3.6146873421963056E-4
12	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	4.337624810635567E-4
13	3.614687342196306E-5	0.0	0.0	3.614687342196306E-5	6.14496848173372E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	25	0.00603228	19.000525	9
GGTATCA	1035	0.0	14.956624	1
GTATCAA	3615	0.0	13.7397785	1
CGGGTTC	320	0.0	13.658851	17
TCGCGCG	140	3.6379788E-12	12.893213	9
TCCAACG	230	0.0	12.8077135	18
AGCCGAC	320	0.0	12.76667	11
GATCGCT	215	0.0	11.93121	11
ATCGCTT	210	0.0	11.763082	12
GACGGGT	360	0.0	11.6131115	15
CGTCGTA	360	0.0	11.611851	10
GTCTTAC	150	1.7826096E-10	11.396398	1
AACGATC	230	0.0	11.152482	8
TAGGTCG	180	3.6379788E-12	11.081835	5
ACCGTAA	95	1.3607294E-5	11.000303	8
GTCCTAT	505	0.0	10.907443	1
TTAGAAC	140	8.401912E-9	10.853712	3
GCGTTAT	175	2.0008883E-11	10.853712	1
CGTGTCG	115	8.820916E-7	10.739428	9
ACCGACA	390	0.0	10.716499	5
>>END_MODULE