FastQCFastQC Report
Thu 2 Feb 2017
SRR4063910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766491
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.030.040741
GTATCAA34050.029.2046761
CGCGCGA808.9963185E-519.24932710
ATACCCG1605.2750693E-1119.2482855
CGTCCCG2300.019.13184425
ATCGCGT708.132797E-418.8551228
GTAGGAC26350.018.3668163
TGTAGGA25900.018.3465182
TTAGGAC14650.018.3195173
GTCCTAC21050.018.2901381
AGGACGT25350.018.2232275
TAGGACG25750.018.1964364
GACGTGA13700.017.662347
CGCTAAC1002.4856356E-517.59938414
TAGGACC17700.017.523864
CTGTAGG27250.017.3581331
TATCAAC57250.017.3300882
TCAACGC57100.017.1438314
GGACGTG26900.017.0914136
ATCAACG57600.017.0335123