##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2766491
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325253181738166	32.0	32.0	32.0	32.0	32.0
2	31.367428630709444	32.0	32.0	32.0	32.0	32.0
3	31.463399664050957	32.0	32.0	32.0	32.0	32.0
4	31.551115835909098	32.0	32.0	32.0	32.0	32.0
5	31.490418367527674	32.0	32.0	32.0	32.0	32.0
6	35.08391352077415	36.0	36.0	36.0	36.0	36.0
7	35.10427577751021	36.0	36.0	36.0	36.0	36.0
8	35.04332781129597	36.0	36.0	36.0	36.0	36.0
9	35.167469187501425	36.0	36.0	36.0	36.0	36.0
10	35.01281623544049	36.0	36.0	36.0	36.0	36.0
11	35.16680336209299	36.0	36.0	36.0	36.0	36.0
12	35.076358462760226	36.0	36.0	36.0	36.0	36.0
13	35.1119540963625	36.0	36.0	36.0	36.0	36.0
14	35.053971619643804	36.0	36.0	36.0	36.0	36.0
15	35.01972534882636	36.0	36.0	36.0	36.0	36.0
16	35.043264915736216	36.0	36.0	36.0	36.0	36.0
17	35.00415363722492	36.0	36.0	36.0	36.0	36.0
18	35.00590820646082	36.0	36.0	36.0	36.0	36.0
19	34.99677859064064	36.0	36.0	36.0	36.0	36.0
20	34.99303269014791	36.0	36.0	36.0	36.0	36.0
21	34.98565005272022	36.0	36.0	36.0	36.0	36.0
22	34.95976780694389	36.0	36.0	36.0	36.0	36.0
23	34.91476061190873	36.0	36.0	36.0	32.0	36.0
24	34.88425734983414	36.0	36.0	36.0	32.0	36.0
25	34.84732211310284	36.0	36.0	36.0	32.0	36.0
26	34.79927315866923	36.0	36.0	36.0	32.0	36.0
27	34.77549538386353	36.0	36.0	36.0	32.0	36.0
28	34.758611902225596	36.0	36.0	36.0	32.0	36.0
29	34.724920124446456	36.0	36.0	36.0	32.0	36.0
30	34.70407422254401	36.0	36.0	36.0	32.0	36.0
31	34.698843047022386	36.0	36.0	36.0	32.0	36.0
32	34.65971008038703	36.0	36.0	36.0	32.0	36.0
33	34.63812967401665	36.0	36.0	36.0	32.0	36.0
34	34.64089780158331	36.0	36.0	36.0	32.0	36.0
35	34.60691757175425	36.0	36.0	36.0	32.0	36.0
36	34.57848697140168	36.0	36.0	36.0	32.0	36.0
37	34.553784198105106	36.0	36.0	36.0	32.0	36.0
38	34.51409456961906	36.0	36.0	36.0	32.0	36.0
39	34.488831881253184	36.0	36.0	36.0	32.0	36.0
40	34.48287885266932	36.0	36.0	36.0	32.0	36.0
41	34.46300313284952	36.0	36.0	36.0	32.0	36.0
42	34.422795158198596	36.0	36.0	36.0	32.0	36.0
43	34.41033894561739	36.0	36.0	36.0	32.0	36.0
44	34.35223429246652	36.0	36.0	36.0	32.0	36.0
45	34.3297946749149	36.0	36.0	36.0	32.0	36.0
46	34.29388781673246	36.0	36.0	36.0	32.0	36.0
47	34.275325674292816	36.0	36.0	36.0	32.0	36.0
48	34.23466839400526	36.0	36.0	36.0	32.0	36.0
49	34.23445657332701	36.0	36.0	36.0	32.0	36.0
50	33.64411704213027	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	4.0
18	7.0
19	14.0
20	39.0
21	80.0
22	258.0
23	791.0
24	2152.0
25	5020.0
26	10013.0
27	18696.0
28	31120.0
29	47619.0
30	69631.0
31	99338.0
32	145231.0
33	238654.0
34	501382.0
35	1596440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74248998097778	18.34082967553768	12.705378206792039	26.211302136692506
2	15.514286044773208	20.270445143930402	38.132483347045124	26.082785464251273
3	18.18625769434722	24.284406279606678	28.749865804150332	28.779470221895775
4	12.47743079590716	16.47444361828757	36.10566598626202	34.94245959954325
5	14.386817090675516	36.94391198091734	33.434701215366324	15.234569713040816
6	33.70715360054886	36.10948349184397	16.864637783523545	13.318725124083628
7	30.318873604367703	30.39677020106359	20.565532906703375	18.718823287865337
8	27.79894378797682	33.90252630589664	18.875072745609057	19.42345716051748
9	27.314769798114053	14.383564862425416	18.352893206957766	39.94877213250277
10	15.883561109054737	27.03516112987554	31.435941331605978	25.64533642946375
11	37.02552224136803	20.750597598616874	23.100507538616636	19.123372621398456
12	25.350128158925017	23.19165063851737	29.21018605907058	22.24803514348703
13	29.82909310288862	20.071809742137745	24.991460257938165	25.107636897035473
14	23.502331299342885	19.80323031083148	25.047686900660516	31.646751489165126
15	25.16415096249362	28.373860775691924	21.586628763615384	24.875359498199074
16	25.38972103759815	25.818282241675846	24.353489253075946	24.438507467650066
17	23.824621153656384	26.339648312609725	25.163212170218518	24.67251836351537
18	24.468106256210504	25.35912926267756	26.577210125636054	23.595554355475887
19	26.13991013884019	24.487382929271387	24.919446663461642	24.45326026842678
20	26.443031330924487	24.39355886278099	24.880822767514392	24.282587038780136
21	26.71391304002073	23.777304896347033	24.421153005739036	25.087629057893196
22	26.235003114053146	24.820286782064354	24.591043310822265	24.35366679306023
23	24.66135446646294	25.024092143683884	24.993909187980893	25.320644201872284
24	25.399831337957018	24.74759432477167	25.074833389300988	24.777740947970326
25	25.160594192185275	24.733909403584743	25.249986896540506	24.855509507689472
26	24.383916100094567	25.376561989398283	25.760209142463843	24.47931276804331
27	25.284712431768064	25.309547048404006	24.944691465133932	24.461049054693994
28	24.41747060422559	25.04746268052447	25.12720576752902	25.407860947720923
29	24.972645220712494	24.652015036001313	24.946004358690832	25.429335384595365
30	24.625520085888727	24.96180915784946	25.333379121375664	25.079291634886154
31	25.355000591050093	24.46437666989726	24.68677056375334	25.493852175299313
32	24.969527183342972	25.300173510699825	24.53256940427993	25.197729901677267
33	24.3216835341319	25.331345651035235	25.35252863769899	24.99444217713387
34	25.251119137541135	24.83064730684033	25.47556115927612	24.442672396342417
35	25.193742546799502	24.958674160804417	25.22855276603882	24.619030526357264
36	24.56620829593308	25.06619980066856	25.190845213749892	25.176746689648468
37	25.603505521116233	24.717798971146035	24.380137356450433	25.2985581512873
38	25.03348380107025	24.890981081697582	24.875617394296928	25.19991772293524
39	25.85109974761076	24.305568107138544	24.823938443341834	25.01939370190886
40	26.158951888034437	24.655349039300585	24.946483219118416	24.23921585354657
41	25.28856998264893	24.738288027761712	25.68301764025448	24.290124349334878
42	25.746546912147284	25.239432526386828	24.976242003703682	24.037778557762206
43	24.697183569526956	24.7634780376982	25.466004203951282	25.073334188823566
44	24.45828569404316	25.076370466254755	25.155063404385913	25.310280435316173
45	24.728514883695713	25.40609806792098	24.96195485188599	24.903432196497317
46	25.03523454695367	24.61502258661879	25.082370581041992	25.26737228538555
47	25.342843076229038	24.68835240848662	25.069674735561335	24.899129779723005
48	25.020450522387495	25.103553926662965	24.46428306776271	25.411712483186832
49	25.041975738836257	24.821948765922315	24.916726279143994	25.21934921609743
50	24.733593402080935	25.778904935137042	24.353430825376076	25.134070837405943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	20.0
2	26.0
3	90.0
4	154.0
5	179.0
6	204.0
7	206.5
8	209.0
9	281.5
10	354.0
11	522.5
12	691.0
13	1300.5
14	1910.0
15	2517.0
16	3124.0
17	3176.0
18	3228.0
19	3288.0
20	3348.0
21	4030.5
22	4713.0
23	5956.5
24	7200.0
25	9763.5
26	12327.0
27	17560.0
28	22793.0
29	29540.0
30	36287.0
31	44093.0
32	51899.0
33	63432.0
34	74965.0
35	89211.5
36	103458.0
37	112654.0
38	121850.0
39	127328.0
40	132806.0
41	139917.5
42	147029.0
43	148873.5
44	150718.0
45	159796.5
46	168875.0
47	178461.5
48	188048.0
49	198944.0
50	209840.0
51	209424.0
52	209008.0
53	205912.5
54	202817.0
55	196706.5
56	190596.0
57	182695.5
58	174795.0
59	159930.0
60	145065.0
61	128975.0
62	112885.0
63	97704.5
64	82524.0
65	70081.5
66	57639.0
67	51158.5
68	44678.0
69	39209.0
70	33740.0
71	26927.5
72	20115.0
73	18993.0
74	17871.0
75	14514.5
76	11158.0
77	8904.0
78	6650.0
79	5070.0
80	3490.0
81	3246.0
82	3002.0
83	2611.0
84	2220.0
85	1541.0
86	862.0
87	663.0
88	464.0
89	397.5
90	331.0
91	264.5
92	198.0
93	145.5
94	93.0
95	87.5
96	82.0
97	88.5
98	95.0
99	69.0
100	43.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009145158975756653
2	5.422031013294459E-4
3	4.337624810635567E-4
4	0.0
5	0.0
6	3.6146873421963056E-4
7	1.0844062026588917E-4
8	3.614687342196306E-5
9	0.0010121124558149655
10	9.759655823930025E-4
11	7.229374684392612E-5
12	1.4458749368785224E-4
13	5.060562279074828E-4
14	2.891749873757045E-4
15	0.004879827911965013
16	9.036718355490765E-4
17	0.0
18	2.891749873757045E-4
19	3.614687342196306E-5
20	8.313780887051503E-4
21	0.0
22	0.0
23	0.0010482593292369287
24	4.337624810635567E-4
25	0.0016627561774103007
26	0.00531359039302857
27	0.006904052823594944
28	0.003867715456150047
29	0.002313399899005636
30	0.00437377168405753
31	0.008241487140207577
32	0.0032893654813986387
33	0.004337624810635567
34	0.002711015506647229
35	0.0022411061521617095
36	0.008819837114958986
37	0.004048449823259862
38	0.008241487140207577
39	0.004662946671433235
40	0.002494134266115451
41	0.0032893654813986387
42	0.001951931164786005
43	0.0016627561774103007
44	0.0015181686837224485
45	0.0014820218103004854
46	0.0010844062026588918
47	0.002349546772427599
48	0.0020965186584738574
49	4.337624810635567E-4
50	0.0015543155571444115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2766491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.04893080202752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37319751006743	33.58032630770605
2	15.108633293235824	14.216900846533228
3	5.6457637504746625	7.968814440620332
4	2.652983957312192	4.992802345058819
5	1.4191991407119398	3.3385901082826486
6	0.8289039472035816	2.339942667210527
7	0.5533328402885586	1.8223602959258016
8	0.38116527199158196	1.434673480485435
9	0.27932767091073973	1.1827861433793818
>10	1.5407782933511878	13.423880644800093
>50	0.12429438100401505	4.0354632031612265
>100	0.08238327418358565	7.612984661719422
>500	0.0071800787816866316	2.3635065097732366
>1k	0.0028565904830228	1.6869683453438817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.229374684392612E-5
2	0.0	0.0	0.0	0.0	7.229374684392612E-5
3	0.0	0.0	0.0	0.0	7.229374684392612E-5
4	0.0	0.0	0.0	0.0	7.229374684392612E-5
5	0.0	0.0	0.0	0.0	1.0844062026588917E-4
6	0.0	0.0	0.0	0.0	1.4458749368785224E-4
7	0.0	0.0	0.0	0.0	1.4458749368785224E-4
8	0.0	0.0	0.0	0.0	1.4458749368785224E-4
9	0.0	0.0	0.0	3.614687342196306E-5	1.4458749368785224E-4
10	0.0	0.0	0.0	7.229374684392612E-5	1.4458749368785224E-4
11	0.0	0.0	0.0	7.229374684392612E-5	1.4458749368785224E-4
12	0.0	0.0	3.614687342196306E-5	7.229374684392612E-5	2.891749873757045E-4
13	0.0	0.0	3.614687342196306E-5	7.229374684392612E-5	3.253218607976675E-4
14	0.0	0.0	3.614687342196306E-5	7.229374684392612E-5	3.253218607976675E-4
15	0.0	0.0	3.614687342196306E-5	1.0844062026588917E-4	7.952312152831873E-4
16	0.0	0.0	3.614687342196306E-5	1.8073436710981528E-4	9.036718355490764E-4
17	0.0	0.0	3.614687342196306E-5	1.8073436710981528E-4	9.036718355490764E-4
18	0.0	0.0	3.614687342196306E-5	2.1688124053177835E-4	9.759655823930025E-4
19	0.0	0.0	3.614687342196306E-5	2.530281139537414E-4	0.0010121124558149655
20	0.0	0.0	3.614687342196306E-5	3.6146873421963056E-4	0.001156699949502818
21	0.0	0.0	3.614687342196306E-5	5.060562279074828E-4	0.001228993696346744
22	0.0	0.0	3.614687342196306E-5	6.14496848173372E-4	0.001228993696346744
23	0.0	0.0	3.614687342196306E-5	9.036718355490764E-4	0.0013735811900345962
24	0.0	0.0	3.614687342196306E-5	0.001156699949502818	0.0013735811900345962
25	0.0	0.0	3.614687342196306E-5	0.0015904624305663746	0.0013735811900345962
26	0.0	0.0	3.614687342196306E-5	0.001879637417942079	0.0014097280634565593
27	0.0	0.0	3.614687342196306E-5	0.001988078038207968	0.0014097280634565593
28	0.0	0.0	7.229374684392612E-5	0.0030363373674448966	0.0014458749368785222
29	0.0	0.0	7.229374684392612E-5	0.004482212304323419	0.0014458749368785222
30	0.0	0.0	7.229374684392612E-5	0.008133046519941688	0.0014458749368785222
31	0.0	0.0	7.229374684392612E-5	0.019302430407328274	0.0015543155571444115
32	0.0	0.0	7.229374684392612E-5	0.031845395484749454	0.0016266093039883375
33	0.0	0.0	7.229374684392612E-5	0.04558120738509541	0.0016266093039883375
34	0.0	0.0	7.229374684392612E-5	0.06123280357680542	0.0016989030508322636
35	0.0	0.0	7.229374684392612E-5	0.07789651222433039	0.0016989030508322636
36	0.0	0.0	7.229374684392612E-5	0.10334391111339238	0.0016989030508322636
37	0.0	0.0	7.229374684392612E-5	0.14494896242207186	0.0017350499242542268
38	0.0	0.0	7.229374684392612E-5	0.20603717850518943	0.001843490544520116
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1245	0.0	30.04074	1
GTATCAA	3405	0.0	29.204676	1
CGCGCGA	80	8.9963185E-5	19.249327	10
ATACCCG	160	5.2750693E-11	19.248285	5
CGTCCCG	230	0.0	19.131844	25
ATCGCGT	70	8.132797E-4	18.855122	8
GTAGGAC	2635	0.0	18.366816	3
TGTAGGA	2590	0.0	18.346518	2
TTAGGAC	1465	0.0	18.319517	3
GTCCTAC	2105	0.0	18.290138	1
AGGACGT	2535	0.0	18.223227	5
TAGGACG	2575	0.0	18.196436	4
GACGTGA	1370	0.0	17.66234	7
CGCTAAC	100	2.4856356E-5	17.599384	14
TAGGACC	1770	0.0	17.52386	4
CTGTAGG	2725	0.0	17.358133	1
TATCAAC	5725	0.0	17.330088	2
TCAACGC	5710	0.0	17.143831	4
GGACGTG	2690	0.0	17.091413	6
ATCAACG	5760	0.0	17.033512	3
>>END_MODULE