FastQCFastQC Report
Thu 2 Feb 2017
SRR4063909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701876
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40400.2373850973866486No Hit
TATCAACGCAGAGTACTTTTTTTTT28770.1690487438567792No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17770.10441418763764224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.00527585214.25143310
GTACTAG957.424023E-812.9951941
GCTCGTA751.47724895E-512.667949
AACCGCG751.4776957E-512.6675687
CGCCGGT1900.012.5008917
CCGACCA1457.2759576E-1212.4495289
TATACTC1052.7208443E-711.76170355
CCGGTCC2600.011.3280639
TACGCTA1604.5474735E-1111.2823859
CGACCAT1303.2610842E-810.96264210
TCCAACG1752.0008883E-1110.85823418
ATTATAC1502.1445885E-910.7630023
CGGTCCA2400.010.68857610
CGCGTAA803.7675645E-410.68857510
TCGCGTA803.7675645E-410.6885759
AATTCCG803.7716617E-410.6873195
GTCCTAA4650.010.6197291
ACGCTAT1701.382432E-1010.61871510
CGCGGTC909.5056675E-510.55661810
CAGGACT3900.010.4720264
Produced by FastQC (version 0.11.5)