##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063909_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701876
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286715365866844	32.0	32.0	32.0	32.0	32.0
2	31.016037596158593	32.0	32.0	32.0	32.0	32.0
3	31.02861078010384	32.0	32.0	32.0	32.0	32.0
4	31.041478932660194	32.0	32.0	32.0	32.0	32.0
5	30.991887188020748	32.0	32.0	32.0	32.0	32.0
6	34.54128209105716	36.0	36.0	36.0	32.0	36.0
7	34.51024398957386	36.0	36.0	36.0	32.0	36.0
8	34.47708822499406	36.0	36.0	36.0	32.0	36.0
9	34.605075222871704	36.0	36.0	36.0	32.0	36.0
10	34.391026138214535	36.0	36.0	36.0	32.0	36.0
11	34.561000331399	36.0	36.0	36.0	32.0	36.0
12	34.44248523394184	36.0	36.0	36.0	32.0	36.0
13	34.50207006855964	36.0	36.0	36.0	32.0	36.0
14	34.42846717387166	36.0	36.0	36.0	32.0	36.0
15	34.37100411545847	36.0	36.0	36.0	32.0	36.0
16	34.37116100115402	36.0	36.0	36.0	32.0	36.0
17	34.3202530619152	36.0	36.0	36.0	32.0	36.0
18	34.310475028732995	36.0	36.0	36.0	32.0	36.0
19	34.3097358444446	36.0	36.0	36.0	32.0	36.0
20	34.27595253708261	36.0	36.0	36.0	32.0	36.0
21	34.24534572436535	36.0	36.0	36.0	32.0	36.0
22	34.22593479195899	36.0	36.0	36.0	32.0	36.0
23	34.18772754301723	36.0	36.0	36.0	32.0	36.0
24	34.163753998528684	36.0	36.0	36.0	32.0	36.0
25	33.65893637374286	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	10.0
5	22.0
6	65.0
7	29.0
8	95.0
9	119.0
10	152.0
11	47.0
12	103.0
13	89.0
14	203.0
15	394.0
16	678.0
17	906.0
18	1131.0
19	1440.0
20	1787.0
21	2495.0
22	3398.0
23	4777.0
24	6873.0
25	9706.0
26	13705.0
27	18505.0
28	26264.0
29	37316.0
30	52158.0
31	77093.0
32	117969.0
33	183354.0
34	419624.0
35	721365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32854867246234	17.453666966442714	11.546726856995068	25.671057504099874
2	16.364545560849947	19.66986739342614	38.5255385324136	25.44004851331031
3	18.582664210523223	24.199829010109557	28.789232925249213	28.428273854118007
4	12.266477535481195	15.98121302114152	36.30331580892658	35.44899363445071
5	14.078941028109107	37.145462900607974	34.275670701393764	14.49992536988916
6	33.66185818797906	35.95404922407526	17.28866210206935	13.09543048587633
7	29.95723157775337	30.71292262247949	20.99196644821095	18.337879351556197
8	27.879749718595058	33.86963383560074	19.17074446668254	19.079871979121663
9	27.507934800308746	14.130029023233284	18.273232254647272	40.088803921810694
10	15.174634492781571	27.605995329597327	32.69593468020911	24.523435497411995
11	36.66552025185851	20.89020579615569	23.100493546272535	19.34378040571326
12	24.53817945782718	23.694587855408823	29.76223863388489	22.004994052879105
13	29.912922818219766	20.071790168098357	25.069350125529898	24.945936888151977
14	23.13044725798931	19.82300656008175	25.98743724059101	31.05910894133792
15	24.80651419836557	27.90469751308131	22.56799341525075	24.720794873302367
16	25.00956920075566	25.93814915488229	24.63620867972076	24.416072964641288
17	23.560337478319664	26.177852250345417	25.85448452244466	24.40732574889026
18	23.984205481626965	25.63633797681981	26.80499120893337	23.574465332619855
19	25.237157508571656	25.239921136639065	25.608836083339266	23.914085271450016
20	25.449826267301972	25.069196125369796	25.451178730342978	24.029798876985254
21	26.054211642619713	24.620567307076023	24.90134544178014	24.42387560852413
22	25.318753458319932	25.053730751748343	25.234254317129924	24.393261472801804
23	24.332491836421468	24.926361669609847	25.654718116175967	25.086428377792714
24	24.53333223569508	25.333863335804978	25.626344729366508	24.506459699133433
25	24.54338416414355	25.16698429797796	25.682564304904894	24.607067232973602
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	130.0
2	355.5
3	581.0
4	581.0
5	581.0
6	1581.5
7	2582.0
8	2582.0
9	2582.0
10	2713.5
11	2845.0
12	2845.0
13	2845.0
14	3629.0
15	4413.0
16	4413.0
17	4413.0
18	7794.0
19	11175.0
20	11175.0
21	11175.0
22	18563.0
23	25951.0
24	25951.0
25	25951.0
26	39428.5
27	52906.0
28	52906.0
29	52906.0
30	68697.5
31	84489.0
32	84489.0
33	84489.0
34	99650.5
35	114812.0
36	114812.0
37	114812.0
38	131208.5
39	147605.0
40	147605.0
41	147605.0
42	166850.5
43	186096.0
44	186096.0
45	186096.0
46	205744.5
47	225393.0
48	225393.0
49	225393.0
50	227886.0
51	230379.0
52	230379.0
53	230379.0
54	214413.5
55	198448.0
56	198448.0
57	198448.0
58	178945.5
59	159443.0
60	159443.0
61	159443.0
62	137695.0
63	115947.0
64	115947.0
65	115947.0
66	93486.0
67	71025.0
68	71025.0
69	71025.0
70	52972.0
71	34919.0
72	34919.0
73	34919.0
74	26071.0
75	17223.0
76	17223.0
77	17223.0
78	13133.5
79	9044.0
80	9044.0
81	9044.0
82	6165.5
83	3287.0
84	3287.0
85	3287.0
86	2444.0
87	1601.0
88	1601.0
89	1601.0
90	1138.5
91	676.0
92	676.0
93	676.0
94	454.0
95	232.0
96	232.0
97	232.0
98	453.0
99	674.0
100	674.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03560776460799729
2	0.004406901560395704
3	0.0012339324369107973
4	0.003466762560844621
5	0.008813803120791408
6	0.013455739431074884
7	0.022739612051641832
8	0.03472638429591815
9	0.04847591716435275
10	0.055938270473289475
11	0.054116751161659246
12	0.06187289790795569
13	0.0638119345945298
14	0.066691110280655
15	0.05734847897261611
16	0.06504586703144061
17	0.060286413346213236
18	0.07556367208891834
19	0.07127428790346653
20	0.07491732652672697
21	0.0731545659025687
22	0.07444725702695144
23	0.07844284777504354
24	0.07374215277728813
25	0.07444725702695144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1701876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.50473612776566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6434731580524	40.87561545169892
2	15.013897013680827	16.666847839876063
3	5.275035716933204	8.7836839659875
4	2.3248666347621927	5.1616443637888825
5	1.1728638645139478	3.254974965681908
6	0.6629389269512899	2.2077750125553273
7	0.4448785002145174	1.7285004634326053
8	0.27294598492091376	1.2119835896134739
9	0.19792908270237236	0.9887401356665296
>10	0.8750309778097909	8.324132920434167
>50	0.0563682979057354	2.1546914807605186
>100	0.05414549162116402	5.961616069529508
>500	0.004670932018738248	1.7466975738225323
>1k	9.554179129237325E-4	0.933096167151991
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4040	0.2373850973866486	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2877	0.1690487438567792	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1777	0.10441418763764224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	5.8758687471942724E-5
11	0.0	0.0	0.0	0.0	5.8758687471942724E-5
12	0.0	0.0	0.0	0.0	4.113108123035991E-4
13	0.0	0.0	0.0	0.0	5.875868747194273E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	40	0.005275852	14.251433	10
GTACTAG	95	7.424023E-8	12.995194	1
GCTCGTA	75	1.47724895E-5	12.66794	9
AACCGCG	75	1.4776957E-5	12.667568	7
CGCCGGT	190	0.0	12.500891	7
CCGACCA	145	7.2759576E-12	12.449528	9
TATACTC	105	2.7208443E-7	11.7617035	5
CCGGTCC	260	0.0	11.328063	9
TACGCTA	160	4.5474735E-11	11.282385	9
CGACCAT	130	3.2610842E-8	10.962642	10
TCCAACG	175	2.0008883E-11	10.858234	18
ATTATAC	150	2.1445885E-9	10.763002	3
CGGTCCA	240	0.0	10.688576	10
CGCGTAA	80	3.7675645E-4	10.688575	10
TCGCGTA	80	3.7675645E-4	10.688575	9
AATTCCG	80	3.7716617E-4	10.687319	5
GTCCTAA	465	0.0	10.619729	1
ACGCTAT	170	1.382432E-10	10.618715	10
CGCGGTC	90	9.5056675E-5	10.556618	10
CAGGACT	390	0.0	10.472026	4
>>END_MODULE