FastQCFastQC Report
Thu 2 Feb 2017
SRR4063909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701876
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2450.025.142158
ATACCGT3000.024.199326
TACCGTC2900.022.7579827
TACACCG1158.780262E-921.0428895
CGTCGTA3000.020.53275710
GTATCAA33900.020.5074021
CGATTAA708.123503E-418.85772333
CCGTCGT3350.018.3875459
ACGATTA851.4312523E-418.11767232
GTCCTAC8100.017.925951
AATACGA2500.017.60054239
GTTATAT1751.9099389E-1017.6000231
TAGGACG7650.017.2544174
TTAGGAC6400.017.1865143
GTATTAA3100.017.032281
TCCTACA9600.016.7282052
GTCGTAG3700.016.64769411
CAATACG2650.016.60428438
CGCGGTT3100.016.32212310
CGCAATA2700.016.29679736
Produced by FastQC (version 0.11.5)