##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701876
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178594092636597	32.0	32.0	32.0	32.0	32.0
2	31.09128867203016	32.0	32.0	32.0	32.0	32.0
3	31.29764154380225	32.0	32.0	32.0	32.0	32.0
4	31.45138247439884	32.0	32.0	32.0	32.0	32.0
5	31.35313501101138	32.0	32.0	32.0	32.0	32.0
6	34.92844484557042	36.0	36.0	36.0	36.0	36.0
7	34.97235344995757	36.0	36.0	36.0	36.0	36.0
8	34.890717655105306	36.0	36.0	36.0	32.0	36.0
9	35.07334611922373	36.0	36.0	36.0	36.0	36.0
10	34.83637938369188	36.0	36.0	36.0	32.0	36.0
11	35.07505070874729	36.0	36.0	36.0	36.0	36.0
12	34.9553998058613	36.0	36.0	36.0	36.0	36.0
13	35.020683645577	36.0	36.0	36.0	36.0	36.0
14	34.947618392879384	36.0	36.0	36.0	32.0	36.0
15	34.90433909403505	36.0	36.0	36.0	32.0	36.0
16	34.92236390900395	36.0	36.0	36.0	32.0	36.0
17	34.89285294580804	36.0	36.0	36.0	32.0	36.0
18	34.878076311082594	36.0	36.0	36.0	32.0	36.0
19	34.88690656663588	36.0	36.0	36.0	32.0	36.0
20	34.88542114701659	36.0	36.0	36.0	32.0	36.0
21	34.874521410490544	36.0	36.0	36.0	32.0	36.0
22	34.85860309446752	36.0	36.0	36.0	32.0	36.0
23	34.80215068547885	36.0	36.0	36.0	32.0	36.0
24	34.790351353447605	36.0	36.0	36.0	32.0	36.0
25	34.75525890252874	36.0	36.0	36.0	32.0	36.0
26	34.68687260411452	36.0	36.0	36.0	32.0	36.0
27	34.68161663952015	36.0	36.0	36.0	32.0	36.0
28	34.65738631956735	36.0	36.0	36.0	32.0	36.0
29	34.62498442894782	36.0	36.0	36.0	32.0	36.0
30	34.60203211044753	36.0	36.0	36.0	32.0	36.0
31	34.60040390721768	36.0	36.0	36.0	32.0	36.0
32	34.553288253668306	36.0	36.0	36.0	32.0	36.0
33	34.518698777114196	36.0	36.0	36.0	32.0	36.0
34	34.5057107568354	36.0	36.0	36.0	32.0	36.0
35	34.46754463897487	36.0	36.0	36.0	32.0	36.0
36	34.44135530438175	36.0	36.0	36.0	32.0	36.0
37	34.435347228587744	36.0	36.0	36.0	32.0	36.0
38	34.39861658546216	36.0	36.0	36.0	32.0	36.0
39	34.38717509383763	36.0	36.0	36.0	32.0	36.0
40	34.377438779323526	36.0	36.0	36.0	32.0	36.0
41	34.35855726269129	36.0	36.0	36.0	32.0	36.0
42	34.29036839346698	36.0	36.0	36.0	32.0	36.0
43	34.282162155174646	36.0	36.0	36.0	32.0	36.0
44	34.223404642876446	36.0	36.0	36.0	32.0	36.0
45	34.17250845537513	36.0	36.0	36.0	32.0	36.0
46	34.153502370325455	36.0	36.0	36.0	32.0	36.0
47	34.12022321250197	36.0	36.0	36.0	32.0	36.0
48	34.07080715633806	36.0	36.0	36.0	32.0	36.0
49	34.04338917758991	36.0	36.0	36.0	32.0	36.0
50	33.37262291729832	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	5.0
20	15.0
21	50.0
22	145.0
23	479.0
24	1311.0
25	2867.0
26	5881.0
27	11334.0
28	19278.0
29	30892.0
30	46099.0
31	68906.0
32	104743.0
33	175596.0
34	360742.0
35	873531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1486918157095	18.34096470217682	12.271734710427161	26.238608771686522
2	15.510553997462786	20.471870010993808	37.92423262217318	26.093343369370224
3	17.831098053617332	24.799941241278002	29.180580242379726	28.188380462724936
4	11.906801670627003	16.761033118746607	36.49032009382587	34.84184511680052
5	13.903539388298562	37.482578049164566	33.82202933703748	14.791853225499391
6	33.886428858506726	36.361286016137484	16.923265854856638	12.829019270499142
7	29.92395441730704	30.810800329519104	20.83896927739656	18.42627597577729
8	27.777346880736314	34.068639548357226	19.00473359986274	19.14927997104372
9	27.45945901479442	13.879477606239767	18.039695438749884	40.62136794021593
10	15.107949600995147	27.755883419249184	32.65840472095514	24.477762258800524
11	36.977018302155976	20.798753845756096	22.910188521372884	19.314039330715048
12	24.503106575457405	23.66262132214253	29.692444916603694	22.141827185796366
13	30.058153643842573	19.987120057865727	25.06306294660406	24.891663351687644
14	23.122876971430887	19.64065473745691	26.028264153670694	31.208204137441513
15	24.901295164917432	27.95660571715328	22.42449278436659	24.7176063335627
16	25.189410187194966	25.88934943021232	24.56603414377899	24.35520623881372
17	23.572633963931565	26.34622028866968	25.708629770911628	24.372515976487126
18	24.13173501399342	25.575087653529554	26.703257767480615	23.589919564996407
19	25.372412561197173	25.17069398710599	25.557091115921487	23.899802335775345
20	25.644733486655234	24.96089586371665	25.477799074662755	23.91657157496536
21	26.284934977636443	24.398311040287307	24.89934636836056	24.41740761371569
22	25.414498714652957	25.02005141388175	25.199911861917002	24.365538009548292
23	24.484404480491133	24.79435819602824	25.600179097426533	25.121058226054092
24	24.718015733300113	25.238150079794675	25.541346332383004	24.502487854522208
25	24.749286508802516	24.97627571991071	25.691911651074744	24.582526120212027
26	24.406628275943117	25.573040310259724	25.88688447526149	24.13344693853567
27	24.741181849058687	25.3516036794545	25.339381139916345	24.56783333157047
28	24.30656428192002	25.324183107927063	25.612933478119842	24.756319132033074
29	24.552778169510646	25.06316692521036	25.413669910214825	24.970384995064165
30	24.287646666165244	25.392990109435527	25.785392614042348	24.533970610356885
31	24.94151591454654	24.95409124163726	25.21588134532495	24.888511498491255
32	24.553246148856054	25.399040563345853	25.241328168702903	24.806385119095186
33	24.267484933670545	25.0806789014977	25.687797918449085	24.96403824638267
34	24.736740976389687	25.28614600306374	25.738362161917593	24.23875085862898
35	24.962055663445636	25.216545669821105	25.59366801129369	24.22773065543957
36	24.29753153961013	25.588379954975732	25.400749464227683	24.71333904118645
37	24.881759009907622	25.346496488173553	25.10628321074896	24.665461291169873
38	24.604185238311544	25.41394323349648	25.40119160178711	24.58067992640487
39	24.98247461389797	25.046347521502017	25.231502564611823	24.73967529998819
40	25.256470447212536	25.200472661269803	25.49456386656192	24.04849302495574
41	24.58424166926191	25.231251065029998	25.82543497294089	24.3590722927672
42	25.16730313053258	25.689616776086744	25.29128578236285	23.851794311017827
43	24.555426546076752	25.273054829550794	25.583598142263504	24.587920482108945
44	24.310841445055935	25.522517154780246	25.207155943680377	24.959485456483442
45	24.37966000508858	25.60638458523641	25.240547193082346	24.77340821659266
46	24.420460341225052	25.16922581539814	25.309483461702083	25.100830381674726
47	24.84688658119462	25.25056659163037	25.35533536289775	24.547211464277257
48	24.901224789111108	25.45909025739139	24.90915736107422	24.730527592423275
49	24.587100762221276	25.636065781835022	25.037546977791465	24.73928647815224
50	24.58027978996949	25.778212860372957	24.94088778786355	24.700619561794003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	22.0
2	27.0
3	76.5
4	126.0
5	136.0
6	146.0
7	162.0
8	178.0
9	239.0
10	300.0
11	505.5
12	711.0
13	1316.0
14	1921.0
15	2650.0
16	3379.0
17	3849.0
18	4319.0
19	4571.0
20	4823.0
21	5384.0
22	5945.0
23	7033.5
24	8122.0
25	9507.0
26	10892.0
27	13973.0
28	17054.0
29	20348.5
30	23643.0
31	27948.0
32	32253.0
33	38127.5
34	44002.0
35	51443.0
36	58884.0
37	64752.0
38	70620.0
39	74828.0
40	79036.0
41	84769.5
42	90503.0
43	93219.0
44	95935.0
45	103771.5
46	111608.0
47	118413.5
48	125219.0
49	128177.0
50	131135.0
51	129027.0
52	126919.0
53	124563.5
54	122208.0
55	119961.0
56	117714.0
57	112975.0
58	108236.0
59	98551.5
60	88867.0
61	78017.5
62	67168.0
63	58250.5
64	49333.0
65	41968.5
66	34604.0
67	29000.0
68	23396.0
69	19960.0
70	16524.0
71	13026.0
72	9528.0
73	7950.0
74	6372.0
75	5066.5
76	3761.0
77	3128.5
78	2496.0
79	2051.5
80	1607.0
81	1316.5
82	1026.0
83	822.5
84	619.0
85	475.5
86	332.0
87	235.0
88	138.0
89	102.0
90	66.0
91	61.0
92	56.0
93	46.0
94	36.0
95	38.5
96	41.0
97	27.5
98	14.0
99	15.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007521111996408669
2	5.288281872474845E-4
3	5.8758687471942724E-5
4	0.0
5	0.0
6	0.0
7	1.1751737494388545E-4
8	0.0
9	0.001057656374494969
10	9.988976870230265E-4
11	0.0
12	1.1751737494388545E-4
13	2.937934373597136E-4
14	1.7627606241582818E-4
15	0.0049944884351151315
16	8.226216246071982E-4
17	0.0
18	2.937934373597136E-4
19	0.0
20	5.875868747194272E-4
21	0.0
22	5.8758687471942724E-5
23	5.288281872474845E-4
24	4.700694997755418E-4
25	0.00193903668657411
26	0.004465660247867647
27	0.005993386122138158
28	0.0035255212483165637
29	0.002115312748989938
30	0.0030554517485410215
31	0.007697388058824498
32	0.0025853822487654802
33	0.003995590748092106
34	0.0020565540615179955
35	0.0024091061863496517
36	0.007462353308936727
37	0.00323172781095685
38	0.007814905433768383
39	0.003466762560844621
40	0.0013514498118546827
41	0.0027028996237093653
42	0.0014689671867985682
43	0.0011751737494388544
44	0.001057656374494969
45	0.0015864845617424535
46	4.700694997755418E-4
47	0.00193903668657411
48	0.0018802779991021672
49	4.700694997755418E-4
50	0.0016452432492143963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1701876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.465832561570096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20694742582906	44.72243743091157
2	14.49193586733334	17.235500635597127
3	4.856637040187234	8.664118951322804
4	2.125389564204947	5.055522398124793
5	1.0682352182848813	3.1761748313450515
6	0.6038465445592809	2.1544942506986926
7	0.3852933087039721	1.6038251167738622
8	0.25567465733173556	1.216312509050064
9	0.17802597249008528	0.9527816404535486
>10	0.740200889848772	7.421837580620489
>50	0.044237377612668424	1.844239793987754
>100	0.04079076531664813	4.798757415587908
>500	0.0026858908582099343	1.0940141868753748
>1k	9.947743919296053E-5	0.05998325865100195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.8758687471942724E-5
7	0.0	5.8758687471942724E-5	0.0	0.0	5.8758687471942724E-5
8	0.0	5.8758687471942724E-5	0.0	0.0	5.8758687471942724E-5
9	0.0	5.8758687471942724E-5	0.0	0.0	5.8758687471942724E-5
10	0.0	5.8758687471942724E-5	0.0	0.0	5.8758687471942724E-5
11	0.0	5.8758687471942724E-5	0.0	0.0	5.8758687471942724E-5
12	0.0	5.8758687471942724E-5	0.0	0.0	3.5255212483165636E-4
13	0.0	1.1751737494388545E-4	0.0	0.0	4.700694997755418E-4
14	0.0	1.1751737494388545E-4	0.0	0.0	4.700694997755418E-4
15	0.0	1.1751737494388545E-4	0.0	0.0	5.875868747194273E-4
16	0.0	1.7627606241582818E-4	0.0	0.0	8.226216246071982E-4
17	0.0	2.350347498877709E-4	0.0	0.0	8.226216246071982E-4
18	0.0	2.350347498877709E-4	0.0	5.8758687471942724E-5	8.813803120791409E-4
19	0.0	2.350347498877709E-4	0.0	5.8758687471942724E-5	8.813803120791409E-4
20	0.0	2.350347498877709E-4	0.0	5.8758687471942724E-5	9.988976870230263E-4
21	0.0	2.350347498877709E-4	0.0	5.8758687471942724E-5	0.001057656374494969
22	0.0	2.350347498877709E-4	0.0	2.9379343735971364E-4	0.0011164150619669118
23	0.0	2.350347498877709E-4	0.0	6.4634556219137E-4	0.0011164150619669118
24	0.0	2.350347498877709E-4	0.0	0.0013514498118546827	0.0011164150619669118
25	0.0	2.350347498877709E-4	0.0	0.001704001936686339	0.0011164150619669118
26	0.0	2.350347498877709E-4	0.0	0.001704001936686339	0.0011751737494388546
27	0.0	2.350347498877709E-4	0.0	0.0022328301239338236	0.0014102084993266254
28	0.0	2.9379343735971364E-4	0.0	0.0033492451859007356	0.0014102084993266254
29	0.0	3.5255212483165636E-4	0.0	0.005405799247418731	0.0014102084993266254
30	0.0	3.5255212483165636E-4	0.0	0.010047735557702206	0.0014102084993266254
31	0.0	3.5255212483165636E-4	0.0	0.024032303176024575	0.0014689671867985682
32	0.0	3.5255212483165636E-4	0.0	0.03643038623260449	0.0014689671867985682
33	0.0	3.5255212483165636E-4	0.0	0.04853467585182469	0.0014689671867985682
34	0.0	3.5255212483165636E-4	0.0	0.06498710834396866	0.0014689671867985682
35	0.0	3.5255212483165636E-4	0.0	0.08396616439740616	0.0014689671867985682
36	0.0	3.5255212483165636E-4	0.0	0.11005502163494872	0.0014689671867985682
37	0.0	3.5255212483165636E-4	0.0	0.14977589436598202	0.0014689671867985682
38	0.0	3.5255212483165636E-4	0.0	0.20594919958915925	0.001527725874270511
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	245	0.0	25.14215	8
ATACCGT	300	0.0	24.19932	6
TACCGTC	290	0.0	22.757982	7
TACACCG	115	8.780262E-9	21.042889	5
CGTCGTA	300	0.0	20.532757	10
GTATCAA	3390	0.0	20.507402	1
CGATTAA	70	8.123503E-4	18.857723	33
CCGTCGT	335	0.0	18.387545	9
ACGATTA	85	1.4312523E-4	18.117672	32
GTCCTAC	810	0.0	17.92595	1
AATACGA	250	0.0	17.600542	39
GTTATAT	175	1.9099389E-10	17.600023	1
TAGGACG	765	0.0	17.254417	4
TTAGGAC	640	0.0	17.186514	3
GTATTAA	310	0.0	17.03228	1
TCCTACA	960	0.0	16.728205	2
GTCGTAG	370	0.0	16.647694	11
CAATACG	265	0.0	16.604284	38
CGCGGTT	310	0.0	16.322123	10
CGCAATA	270	0.0	16.296797	36
>>END_MODULE