FastQCFastQC Report
Thu 2 Feb 2017
SRR4063908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5000202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA471660.9432818914115869No Hit
GTCCTACAGTGGACATTTCTAAATT467000.9339622679243759No Hit
GTCCTAAAGTGTGTATTTCTCATTT461860.9236826832195979No Hit
CTTTAGGACGTGAAATATGGCGAGG400050.8000676772658385No Hit
GTCCTACAGTGTGCATTTCTCATTT275960.5518977033327854No Hit
CTGTAGGACCTGGAATATGGCGAGA203060.40610359341482605No Hit
CTGAAGGACCTGGAATATGGCGAGA200190.40036382530145787No Hit
ATTTAGAAATGTCCACTGTAGGACG187670.3753248368765902No Hit
GTCCTTCAGTGTGCATTTCTCATTT163670.32732677599824966No Hit
TTTCTAAATTTTCCACCTTTTTCAG144640.28926831356013216No Hit
GATATACACTGTTCTACAAATCCCG132360.26470930574404794No Hit
GAATATGGCAAGAAAACTGAAAATC131070.26212940997183715No Hit
TTGTAGAACAGTGTATATCAATGAG118250.23649044578599024No Hit
GGAATATGGCGAGAAAACTGAAAAT109960.21991111559093013No Hit
ATTCCAGGTCCTTCAGTGTGCATTT101890.20377176762058813No Hit
GCCATATTCCACGTCCTACAGTGGA97860.19571209323143343No Hit
GAAATATGGCGAGGAAAACTGAAAA95260.19051230330294655No Hit
CTGTAGGACATGGAATATGGCAAGA93390.18677245439284254No Hit
GTGTATATCAATGAGTTACAATGAA92840.18567249883104722No Hit
TTCCAGGTCCTTCAGTGTGCATTTC91210.1824126305297266No Hit
ACCTGGAATATGGCGAGAAAACTGA84410.16881317994753012No Hit
GACCTGGAATATGGCGAGAAAACTG84170.16833319933874671No Hit
ACAGTGGACATTTCTAAATTTTCCA83750.16749323327337576No Hit
CCATATTTCACGTCCTAAAGTGTGT83210.1664132769036131No Hit
GTCCACTGTAGGACGTGGAATATGG81210.16241343849708473No Hit
CCATATTCCAGGTCCTTCAGTGTGC81150.16229344334488888No Hit
GATATACACTGTTCTACAATGCCGG80090.16017352898942883No Hit
ATACACACTTTAGGACGTGAAATAT74110.14821401215390897No Hit
CCACTGTAGGACGTGGAATATGGCA71870.1437341931385972No Hit
CACTTTAGGACGTGAAATATGGCGA70940.1418742682795615No Hit
GTGTATATCAATGAGTTACAATGAG70710.14141428686281074No Hit
CCTAAAGTGTGTATTTCTCATTTTC70710.14141428686281074No Hit
TCCTAAAGTGTGTATTTCTCATTTT70390.1407743127177662No Hit
GTTCTACAGTGTGGTTTTTATCATT68080.13615449935822593No Hit
GTAGGACGTGGAATATGGCAAGAAA65200.13039473205282506No Hit
ATTCCACGTCCTACAGTGGACATTT63230.12645489122239462No Hit
GAAATACACACTTTAGGACGTGAAA61030.1220550689752134No Hit
GTGTATTTCTCATTTTCCGTGATTT60290.1205751287647979No Hit
CTACAGTGGACATTTCTAAATTTTC60250.12049513199666732No Hit
GTTCTACAAATCCCGTTTCCAACGA58880.1177552426881954No Hit
GTCCTACAGTGGACATTTCTAAATA57490.11497535499565817No Hit
CTGTAGAACATATTAGATGAGTGAG56780.11355541236134059No Hit
GTATCAACGCAGAGTACATGGGAAA56170.11233546164734945No Hit
ATATTTCACGTCCTAAAGTGTGTAT50220.10043594238792752No Hit
AGTGTGTATTTCTCATTTTCCGTGA50030.10005595773930732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.015.2824221
GCGTTAT3900.014.6143371
CCGCTAT400.005280623714.2502639
CGTCGTG400.005280623714.2502639
TAGGACC83400.013.8251944
GTATCAA78250.013.5114931
CCACCTT42550.013.28621213
TAGAAAT51800.013.1647714
CCAACGT7350.013.056119
ACCTTTT45600.013.00219215
TCCAACG21000.012.98526318
CCAACGA13700.012.96882119
ACGGTAT7350.012.9254089
CACCTTT44550.012.83929714
AAATGTC52850.012.6544097
AAGACGG8850.012.4495915
ATTTAGA54650.012.3586761
AGGACCT131550.012.339335
GTCCTAG9800.012.3103431
TTTAGAA57600.012.2830052
Produced by FastQC (version 0.11.5)