##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063908_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5000202
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387561742505603	32.0	32.0	32.0	32.0	32.0
2	31.074751180052324	32.0	32.0	32.0	32.0	32.0
3	31.0796841807591	32.0	32.0	32.0	32.0	32.0
4	31.103878803296347	32.0	32.0	32.0	32.0	32.0
5	31.08952638313412	32.0	32.0	32.0	32.0	32.0
6	34.77299577097085	36.0	36.0	36.0	36.0	36.0
7	34.7038771633626	36.0	36.0	36.0	32.0	36.0
8	34.68363078131644	36.0	36.0	36.0	32.0	36.0
9	34.75020369177085	36.0	36.0	36.0	32.0	36.0
10	34.51377104364984	36.0	36.0	36.0	32.0	36.0
11	34.69906055795346	36.0	36.0	36.0	32.0	36.0
12	34.55421880956009	36.0	36.0	36.0	32.0	36.0
13	34.61191447865506	36.0	36.0	36.0	32.0	36.0
14	34.56322164584551	36.0	36.0	36.0	32.0	36.0
15	34.52036517724684	36.0	36.0	36.0	32.0	36.0
16	34.51853465120009	36.0	36.0	36.0	32.0	36.0
17	34.4644772351197	36.0	36.0	36.0	32.0	36.0
18	34.47785169479153	36.0	36.0	36.0	32.0	36.0
19	34.39611719686525	36.0	36.0	36.0	32.0	36.0
20	34.360521234942105	36.0	36.0	36.0	32.0	36.0
21	34.33477607504657	36.0	36.0	36.0	32.0	36.0
22	34.272389995444186	36.0	36.0	36.0	32.0	36.0
23	34.2819212103831	36.0	36.0	36.0	32.0	36.0
24	34.21679144162576	36.0	36.0	36.0	32.0	36.0
25	33.881051005539376	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	44.0
5	71.0
6	201.0
7	57.0
8	300.0
9	367.0
10	452.0
11	111.0
12	297.0
13	301.0
14	378.0
15	630.0
16	1233.0
17	1552.0
18	1965.0
19	2603.0
20	3730.0
21	5546.0
22	8522.0
23	13206.0
24	19955.0
25	29835.0
26	43084.0
27	57733.0
28	80472.0
29	110301.0
30	148231.0
31	206380.0
32	297548.0
33	437981.0
34	1019755.0
35	2507345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.469339198904564	19.915345626010613	12.904990310523415	25.710324864561407
2	11.878506299088187	19.8984785787001	48.22239511353159	20.000620008680123
3	19.19087012450921	25.615526212212846	29.6492358571168	25.544367806161144
4	10.461802092360418	17.319123463824692	39.703407940681586	32.5156665031333
5	10.904473575310062	40.42864654680836	36.3677729448569	12.299106933024676
6	30.398574741464866	39.53334264090469	18.448313176040713	11.619769441589723
7	27.650279454773134	31.146404695320285	22.12285607979164	19.080459770114942
8	22.805864525789044	43.74598365372073	19.15920216456556	14.288949655924664
9	26.848660349292448	14.497345350190871	20.70100603364064	37.95298826687605
10	16.38117360690186	28.542755721584285	33.87519683282502	21.200873838688832
11	34.8038396635702	19.408626496448065	29.077041199483656	16.71049264049808
12	27.517906462336246	22.652247706045642	33.30937199753689	16.520473834081226
13	31.13695149907493	22.87697509363277	25.902241683118483	20.083831724173816
14	18.966189056697797	26.496636316969713	25.9349317707192	28.60224285561329
15	20.02009551875135	40.51692430100934	21.144419981533417	18.3185601987059
16	18.836615433877682	27.033741594620558	34.947106147934676	19.182536823567084
17	18.68956213674244	31.988178438498043	30.33121464983487	18.99104477492465
18	19.123945352264453	26.109128945134096	38.01603532912295	16.750890373478498
19	25.62221426098234	24.092642146492597	26.441559273321154	23.843584319203913
20	25.22514931059626	28.17411239781839	28.73572719999824	17.865011091587107
21	22.54114569073043	26.001662799876392	25.297814903234173	26.159376606159
22	24.07947110531919	32.640944744148136	25.960589092057596	17.31899505847508
23	18.820072967059538	32.074621198397985	29.72764155307306	19.37766428146942
24	24.892043968202533	26.631869881533078	30.600228531535773	17.87585761872862
25	21.076903739126223	29.817997459666294	30.564705854096136	18.540392947111343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	142.0
2	481.0
3	820.0
4	820.0
5	820.0
6	1974.5
7	3129.0
8	3129.0
9	3129.0
10	3939.5
11	4750.0
12	4750.0
13	4750.0
14	7941.5
15	11133.0
16	11133.0
17	11133.0
18	23226.5
19	35320.0
20	35320.0
21	35320.0
22	80508.0
23	125696.0
24	125696.0
25	125696.0
26	225687.0
27	325678.0
28	325678.0
29	325678.0
30	464944.0
31	604210.0
32	604210.0
33	604210.0
34	635575.5
35	666941.0
36	666941.0
37	666941.0
38	661115.5
39	655290.0
40	655290.0
41	655290.0
42	657883.0
43	660476.0
44	660476.0
45	660476.0
46	654247.0
47	648018.0
48	648018.0
49	648018.0
50	561486.5
51	474955.0
52	474955.0
53	474955.0
54	360536.5
55	246118.0
56	246118.0
57	246118.0
58	213780.5
59	181443.0
60	181443.0
61	181443.0
62	159329.0
63	137215.0
64	137215.0
65	137215.0
66	115032.5
67	92850.0
68	92850.0
69	92850.0
70	70480.5
71	48111.0
72	48111.0
73	48111.0
74	40183.0
75	32255.0
76	32255.0
77	32255.0
78	28816.0
79	25377.0
80	25377.0
81	25377.0
82	17140.0
83	8903.0
84	8903.0
85	8903.0
86	7152.5
87	5402.0
88	5402.0
89	5402.0
90	3983.5
91	2565.0
92	2565.0
93	2565.0
94	1838.5
95	1112.0
96	1112.0
97	1112.0
98	1702.5
99	2293.0
100	2293.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03991838729715319
2	0.005439780232878591
3	0.0017999272829377693
4	0.004059835982626302
5	0.009299624295178475
6	0.013759444118457614
7	0.023639044982582704
8	0.03755848263730145
9	0.051117934835432643
10	0.05881762376799977
11	0.05569774981090764
12	0.06525736360251046
13	0.06573734421129386
14	0.06893721493651657
15	0.06135752115614529
16	0.06803725129504769
17	0.06461738945746591
18	0.07967678105804525
19	0.07543695234712518
20	0.08145670914895038
21	0.07619692164436556
22	0.0794167915616209
23	0.08497656694669535
24	0.07909680448909864
25	0.08015676166682867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5000202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.73684330375891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03807468645432	22.756967001495457
2	14.510353023242246	8.920048943152423
3	4.844063870973084	4.466736964664984
4	2.0978043696115343	2.579195367627618
5	1.0425626824504466	1.6022542902412968
6	0.6511325720883735	1.2008255902952303
7	0.4219167670901378	0.9077872690094675
8	0.3004628225182465	0.7388222955478774
9	0.223846445882615	0.6192299818077558
>10	1.4515173306580424	8.748206687316829
>50	0.1861762920931194	4.007807633492468
>100	0.17454323075312655	11.230482902990913
>500	0.031061710728231422	6.656841852539946
>1k	0.023541507072429035	13.998945060862814
>5k	0.001961792256035753	4.391658403388356
>10k+	9.808961280178764E-4	7.174189755566553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	47166	0.9432818914115869	No Hit
GTCCTACAGTGGACATTTCTAAATT	46700	0.9339622679243759	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	46186	0.9236826832195979	No Hit
CTTTAGGACGTGAAATATGGCGAGG	40005	0.8000676772658385	No Hit
GTCCTACAGTGTGCATTTCTCATTT	27596	0.5518977033327854	No Hit
CTGTAGGACCTGGAATATGGCGAGA	20306	0.40610359341482605	No Hit
CTGAAGGACCTGGAATATGGCGAGA	20019	0.40036382530145787	No Hit
ATTTAGAAATGTCCACTGTAGGACG	18767	0.3753248368765902	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	16367	0.32732677599824966	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	14464	0.28926831356013216	No Hit
GATATACACTGTTCTACAAATCCCG	13236	0.26470930574404794	No Hit
GAATATGGCAAGAAAACTGAAAATC	13107	0.26212940997183715	No Hit
TTGTAGAACAGTGTATATCAATGAG	11825	0.23649044578599024	No Hit
GGAATATGGCGAGAAAACTGAAAAT	10996	0.21991111559093013	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	10189	0.20377176762058813	No Hit
GCCATATTCCACGTCCTACAGTGGA	9786	0.19571209323143343	No Hit
GAAATATGGCGAGGAAAACTGAAAA	9526	0.19051230330294655	No Hit
CTGTAGGACATGGAATATGGCAAGA	9339	0.18677245439284254	No Hit
GTGTATATCAATGAGTTACAATGAA	9284	0.18567249883104722	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	9121	0.1824126305297266	No Hit
ACCTGGAATATGGCGAGAAAACTGA	8441	0.16881317994753012	No Hit
GACCTGGAATATGGCGAGAAAACTG	8417	0.16833319933874671	No Hit
ACAGTGGACATTTCTAAATTTTCCA	8375	0.16749323327337576	No Hit
CCATATTTCACGTCCTAAAGTGTGT	8321	0.1664132769036131	No Hit
GTCCACTGTAGGACGTGGAATATGG	8121	0.16241343849708473	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	8115	0.16229344334488888	No Hit
GATATACACTGTTCTACAATGCCGG	8009	0.16017352898942883	No Hit
ATACACACTTTAGGACGTGAAATAT	7411	0.14821401215390897	No Hit
CCACTGTAGGACGTGGAATATGGCA	7187	0.1437341931385972	No Hit
CACTTTAGGACGTGAAATATGGCGA	7094	0.1418742682795615	No Hit
GTGTATATCAATGAGTTACAATGAG	7071	0.14141428686281074	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	7071	0.14141428686281074	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	7039	0.1407743127177662	No Hit
GTTCTACAGTGTGGTTTTTATCATT	6808	0.13615449935822593	No Hit
GTAGGACGTGGAATATGGCAAGAAA	6520	0.13039473205282506	No Hit
ATTCCACGTCCTACAGTGGACATTT	6323	0.12645489122239462	No Hit
GAAATACACACTTTAGGACGTGAAA	6103	0.1220550689752134	No Hit
GTGTATTTCTCATTTTCCGTGATTT	6029	0.1205751287647979	No Hit
CTACAGTGGACATTTCTAAATTTTC	6025	0.12049513199666732	No Hit
GTTCTACAAATCCCGTTTCCAACGA	5888	0.1177552426881954	No Hit
GTCCTACAGTGGACATTTCTAAATA	5749	0.11497535499565817	No Hit
CTGTAGAACATATTAGATGAGTGAG	5678	0.11355541236134059	No Hit
GTATCAACGCAGAGTACATGGGAAA	5617	0.11233546164734945	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	5022	0.10043594238792752	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	5003	0.10005595773930732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.999919203264188E-5	0.0
3	0.0	0.0	0.0	1.999919203264188E-5	0.0
4	0.0	0.0	0.0	1.999919203264188E-5	0.0
5	0.0	0.0	0.0	3.999838406528376E-5	0.0
6	0.0	0.0	0.0	3.999838406528376E-5	0.0
7	0.0	0.0	0.0	5.9997576097925646E-5	0.0
8	0.0	0.0	0.0	5.9997576097925646E-5	0.0
9	0.0	0.0	0.0	5.9997576097925646E-5	0.0
10	0.0	0.0	0.0	5.9997576097925646E-5	0.0
11	0.0	0.0	0.0	5.9997576097925646E-5	0.0
12	0.0	0.0	0.0	5.9997576097925646E-5	0.0
13	0.0	0.0	0.0	5.9997576097925646E-5	1.999919203264188E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2275	0.0	15.282422	1
GCGTTAT	390	0.0	14.614337	1
CCGCTAT	40	0.0052806237	14.250263	9
CGTCGTG	40	0.0052806237	14.250263	9
TAGGACC	8340	0.0	13.825194	4
GTATCAA	7825	0.0	13.511493	1
CCACCTT	4255	0.0	13.286212	13
TAGAAAT	5180	0.0	13.164771	4
CCAACGT	735	0.0	13.0561	19
ACCTTTT	4560	0.0	13.002192	15
TCCAACG	2100	0.0	12.985263	18
CCAACGA	1370	0.0	12.968821	19
ACGGTAT	735	0.0	12.925408	9
CACCTTT	4455	0.0	12.839297	14
AAATGTC	5285	0.0	12.654409	7
AAGACGG	885	0.0	12.449591	5
ATTTAGA	5465	0.0	12.358676	1
AGGACCT	13155	0.0	12.33933	5
GTCCTAG	980	0.0	12.310343	1
TTTAGAA	5760	0.0	12.283005	2
>>END_MODULE