FastQCFastQC Report
Thu 2 Feb 2017
SRR4063908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5000202
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA248070.49611995675374715No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC176540.35306573614425973No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA173330.3466459955017817No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA170600.3411862160768705No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA111050.2220910275224881No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA89840.17967274122125465No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG75840.15167387237555605No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT69340.1386743975543388No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA64040.1280748257770386No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA62870.1257349203092195No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA57180.11435538004264628No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA53680.10735566283122161No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG51970.10393580099363985No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT51160.10231586643899586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81700.029.4343621
GGTATCA28600.028.6945461
TAGGACC97500.026.037364
CTAGGAC14950.022.9548683
TGTAGGA277400.022.9346392
CTGTAGG268700.022.7140881
AGGACCT167000.022.4069945
GGACCTG164850.021.9519276
GTAGGAC285650.021.7558273
TTAGGAC164150.021.281443
TAGGACA65150.021.1038344
ACTGTTC56150.020.8822618
GTCCTAC249600.020.8381611
CTTTAGG169150.020.6164461
GATATAC63300.020.298581
TAGGACG311300.020.2460944
TCCTACA261500.020.240632
TTTAGGA176950.020.1899642
GGACATG65400.020.1485986
AGGACGT309950.020.0929645
Produced by FastQC (version 0.11.5)