##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5000202
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425959191248673	32.0	32.0	32.0	32.0	32.0
2	31.526287737975387	32.0	32.0	32.0	32.0	32.0
3	31.575248359966256	32.0	32.0	32.0	32.0	32.0
4	31.639180777096605	32.0	32.0	32.0	32.0	32.0
5	31.618950594395987	32.0	32.0	32.0	32.0	32.0
6	35.240336690397704	36.0	36.0	36.0	36.0	36.0
7	35.25791058041255	36.0	36.0	36.0	36.0	36.0
8	35.20044170215523	36.0	36.0	36.0	36.0	36.0
9	35.295561259325126	36.0	36.0	36.0	36.0	36.0
10	35.18587649058978	36.0	36.0	36.0	36.0	36.0
11	35.291756813024755	36.0	36.0	36.0	36.0	36.0
12	35.21939613639609	36.0	36.0	36.0	36.0	36.0
13	35.24465031612723	36.0	36.0	36.0	36.0	36.0
14	35.1986231756237	36.0	36.0	36.0	36.0	36.0
15	35.173104206590054	36.0	36.0	36.0	36.0	36.0
16	35.19438074701782	36.0	36.0	36.0	36.0	36.0
17	35.19423555288366	36.0	36.0	36.0	36.0	36.0
18	35.18767541791312	36.0	36.0	36.0	36.0	36.0
19	35.14293082559465	36.0	36.0	36.0	36.0	36.0
20	35.12687787413388	36.0	36.0	36.0	36.0	36.0
21	35.09923579087405	36.0	36.0	36.0	36.0	36.0
22	35.05124132985027	36.0	36.0	36.0	36.0	36.0
23	35.05110693527981	36.0	36.0	36.0	36.0	36.0
24	35.01822566368319	36.0	36.0	36.0	36.0	36.0
25	34.955558395440825	36.0	36.0	36.0	36.0	36.0
26	34.929083065044175	36.0	36.0	36.0	36.0	36.0
27	34.859165889698055	36.0	36.0	36.0	36.0	36.0
28	34.785846451803344	36.0	36.0	36.0	32.0	36.0
29	34.74667763422358	36.0	36.0	36.0	32.0	36.0
30	34.67512492495303	36.0	36.0	36.0	32.0	36.0
31	34.68307700368905	36.0	36.0	36.0	32.0	36.0
32	34.64015753763548	36.0	36.0	36.0	32.0	36.0
33	34.69932774715902	36.0	36.0	36.0	32.0	36.0
34	34.67778841734794	36.0	36.0	36.0	32.0	36.0
35	34.651061097131674	36.0	36.0	36.0	32.0	36.0
36	34.57066594509582	36.0	36.0	36.0	32.0	36.0
37	34.45150855905421	36.0	36.0	36.0	32.0	36.0
38	34.384985046604115	36.0	36.0	36.0	32.0	36.0
39	34.17238523563648	36.0	36.0	36.0	32.0	36.0
40	34.30380572624866	36.0	36.0	36.0	32.0	36.0
41	34.30274616905477	36.0	36.0	36.0	32.0	36.0
42	34.27680261717427	36.0	36.0	36.0	32.0	36.0
43	34.30216999233231	36.0	36.0	36.0	32.0	36.0
44	34.139602560056574	36.0	36.0	36.0	32.0	36.0
45	34.11750365285243	36.0	36.0	36.0	32.0	36.0
46	33.92241393447705	36.0	36.0	36.0	32.0	36.0
47	33.97671074088607	36.0	36.0	36.0	32.0	36.0
48	33.915611609290984	36.0	36.0	36.0	32.0	36.0
49	33.87214976514949	36.0	36.0	36.0	32.0	36.0
50	33.42935385410429	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	9.0
20	47.0
21	128.0
22	380.0
23	1203.0
24	3039.0
25	7523.0
26	15970.0
27	30985.0
28	54427.0
29	85867.0
30	127092.0
31	183439.0
32	266205.0
33	433017.0
34	895106.0
35	2895760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6426414173537	20.777320420661873	13.437798765535808	26.142239396448613
2	11.448277020648872	20.563456166342945	47.493408232030234	20.494858580977947
3	18.711512704819757	26.227208611514484	29.993746236392266	25.067532447273493
4	10.328622723641963	17.860158449598636	39.94742612398459	31.863792702774806
5	10.613371219802719	40.94350588236235	35.97472662104451	12.468396276790418
6	30.633020451639197	39.549288766457884	18.222282131104723	11.595408650798191
7	27.68792909559363	31.290507154018133	22.224384359256245	18.79717939113199
8	22.930302787888486	43.71521139154434	19.025118995240188	14.329366825326986
9	26.570606651256423	14.385142970054027	20.757067104947904	38.28718327374164
10	16.436517042578497	28.9069354477753	33.43859677893856	21.217950730707642
11	34.627467975787376	19.448798158314034	29.262663640921634	16.661070224976957
12	27.517892305778535	22.801815289566765	33.10471553703716	16.575576867617535
13	31.062100401645782	22.828891857228253	26.003939460543098	20.105068280582866
14	19.01496123446534	26.598874562541535	25.86516229686518	28.521001906127946
15	20.05680853400022	40.50000070000658	21.082318173790835	18.36087259220237
16	18.963860742000552	27.243199114907966	34.48921014095955	19.303730002131932
17	18.772321598207434	32.315994433824876	29.88719255742068	19.024491410547014
18	19.257862865364274	26.287528391472232	37.67437618758201	16.780232555581488
19	25.853611026281154	24.16065840579545	26.52894414802291	23.45678641990049
20	25.550846601723503	28.286455232395234	28.437070201855256	17.725627964026003
21	22.707622612046475	25.87305472858896	25.466931135982108	25.952391523382456
22	24.336925462062258	32.278140519404054	26.09067811796027	17.294255900573418
23	18.848280624676136	32.07671996030526	29.56383986989214	19.511159545126468
24	25.117810735251382	26.682034110365205	30.24862499977501	17.951530154608403
25	21.303508015452515	29.878770230627755	30.14168539298877	18.676036360930958
26	16.88117009872158	32.60766172258756	31.80026880430087	18.710899374389992
27	19.36340408510213	30.736128403210078	29.813405335133382	20.08706217655441
28	17.933650760190456	31.963899178339506	27.408136444881865	22.69431361658817
29	21.00995224474498	27.397652596619494	26.755122620087125	24.8372725385484
30	18.04652556277955	28.772536981970415	30.223375821299342	22.957561633950693
31	24.14724861874917	26.084219899292293	28.239039196642434	21.5294922853161
32	20.029663827949797	30.912440707843896	28.40717523838362	20.650720225822692
33	17.760539844858602	31.540523864714782	32.01910817197355	18.679828118453067
34	20.361988235322354	30.68086091904644	31.303194446777756	17.653956398853452
35	19.029511569809756	31.717272605273678	30.647280951208582	18.605934873707987
36	17.237255700423457	30.19356358769264	32.3559883547435	20.2131923571404
37	19.104084716732224	29.88645609083513	26.989438408449274	24.020020783983373
38	20.54759423232583	32.69156727763147	26.93979375562648	19.82104473441622
39	23.908825198830876	26.78417785038254	28.27842442506747	21.028572525719113
40	25.80106654208209	26.945047323204967	29.721340681476548	17.532545453236388
41	23.91690433238267	25.4627981488074	32.197271210915154	18.42302630789477
42	21.77201585087664	31.89102942299977	29.98464831317441	16.352306412949176
43	18.09588550969589	31.914464264679033	31.169940495297205	18.81970973032788
44	17.326859105547793	31.740995689514662	29.39625805953562	21.535887145401933
45	18.129052270451425	32.175309877005176	28.727118840333922	20.968519012209484
46	19.71516488685553	27.036276468174194	29.774011050855215	23.474547594115062
47	24.17234840795579	27.16677989816565	28.70129503902037	19.95957665485819
48	19.3569838314975	29.443718235584466	27.806548359510185	23.39274957340785
49	22.635597955756044	27.812805363640475	28.18799140671967	21.363605273883813
50	19.343144815492725	33.347186312030416	26.40724397455425	20.902424897922614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	8.5
2	14.0
3	36.0
4	58.0
5	97.5
6	137.0
7	154.0
8	171.0
9	245.0
10	319.0
11	522.5
12	726.0
13	1417.5
14	2109.0
15	3138.5
16	4168.0
17	4792.0
18	5416.0
19	6344.0
20	7272.0
21	9244.0
22	11216.0
23	14871.5
24	18527.0
25	29815.0
26	41103.0
27	73043.5
28	104984.0
29	144099.5
30	183215.0
31	236028.0
32	288841.0
33	377507.0
34	466173.0
35	561557.0
36	656941.0
37	642357.0
38	627773.0
39	550840.0
40	473907.0
41	404025.0
42	334143.0
43	285382.0
44	236621.0
45	225047.0
46	213473.0
47	212228.5
48	210984.0
49	205440.5
50	199897.0
51	175013.0
52	150129.0
53	139736.5
54	129344.0
55	131388.5
56	133433.0
57	128109.5
58	122786.0
59	110096.5
60	97407.0
61	85004.0
62	72601.0
63	63010.0
64	53419.0
65	46495.0
66	39571.0
67	35803.5
68	32036.0
69	32187.0
70	32338.0
71	23884.5
72	15431.0
73	13963.5
74	12496.0
75	9332.0
76	6168.0
77	5731.5
78	5295.0
79	4475.5
80	3656.0
81	3005.5
82	2355.0
83	2084.5
84	1814.0
85	1301.5
86	789.0
87	584.5
88	380.0
89	294.5
90	209.0
91	172.5
92	136.0
93	106.0
94	76.0
95	73.0
96	70.0
97	48.5
98	27.0
99	36.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00923962671908055
2	5.199789928486889E-4
3	2.5998949642434447E-4
4	0.0
5	0.0
6	3.1998707252227013E-4
7	9.999596016320942E-5
8	3.9998384065283766E-5
9	0.0012799482900890805
10	9.799604095994522E-4
11	1.9999192032641883E-5
12	1.5999353626113506E-4
13	4.999798008160469E-4
14	2.5998949642434447E-4
15	0.004979798816127828
16	8.799644494362428E-4
17	0.0
18	4.199830326854795E-4
19	5.999757609792564E-5
20	6.999717211424658E-4
21	0.0
22	1.9999192032641884E-4
23	8.599652574036009E-4
24	4.199830326854795E-4
25	0.002199911123590607
26	0.005639772153205011
27	0.006539735794673895
28	0.004099834366691586
29	0.0024798998120475935
30	0.003959840022463092
31	0.007719688124599767
32	0.0034598602216470453
33	0.004939800432062545
34	0.002999878804896282
35	0.0032598683013206264
36	0.007959678428991469
37	0.003959840022463092
38	0.008099672773219962
39	0.004479819015311782
40	0.0023599046598517417
41	0.003639852949940823
42	0.0019999192032641878
43	0.001859924859035695
44	0.0013799442502522897
45	0.0014599410183828575
46	9.199628335015266E-4
47	0.0024199022359496676
48	0.002199911123590607
49	3.1998707252227013E-4
50	0.0014799402104154994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5000202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.47432509597016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6355738869814	32.1256995409592
2	13.830724274768619	11.749013583184968
3	4.53920538144878	5.783990551470981
4	2.023857350865877	3.4384790027418477
5	1.028275322096282	2.1837650159440454
6	0.6080008738534721	1.5494656064811794
7	0.4027119163271488	1.1973441797870317
8	0.2892284510499794	0.9827826605520574
9	0.2031462231712104	0.7765648852493248
>10	1.178013862786846	9.474213289963984
>50	0.12471201076770043	3.7279796504804277
>100	0.10735797312258878	9.501188983981573
>500	0.01647120040574831	4.818007367314274
>1k	0.012056728276117512	9.756983227303403
>5k	4.272069074214836E-4	1.1611395126417121
>10k+	2.373371707897131E-4	1.7733829419442344
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	24807	0.49611995675374715	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	17654	0.35306573614425973	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	17333	0.3466459955017817	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	17060	0.3411862160768705	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	11105	0.2220910275224881	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	8984	0.17967274122125465	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	7584	0.15167387237555605	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	6934	0.1386743975543388	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	6404	0.1280748257770386	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	6287	0.1257349203092195	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5718	0.11435538004264628	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	5368	0.10735566283122161	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	5197	0.10393580099363985	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	5116	0.10231586643899586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.9997576097925646E-5
2	0.0	0.0	0.0	0.0	5.9997576097925646E-5
3	0.0	0.0	0.0	0.0	5.9997576097925646E-5
4	0.0	0.0	0.0	0.0	5.9997576097925646E-5
5	0.0	0.0	0.0	0.0	7.999676813056752E-5
6	0.0	0.0	0.0	0.0	7.999676813056752E-5
7	0.0	0.0	0.0	0.0	7.999676813056752E-5
8	0.0	0.0	0.0	0.0	7.999676813056752E-5
9	0.0	0.0	0.0	0.0	7.999676813056752E-5
10	0.0	0.0	0.0	0.0	7.999676813056752E-5
11	0.0	0.0	0.0	0.0	7.999676813056752E-5
12	0.0	0.0	0.0	0.0	9.99959601632094E-5
13	0.0	0.0	0.0	0.0	9.99959601632094E-5
14	0.0	0.0	0.0	0.0	9.99959601632094E-5
15	0.0	0.0	0.0	1.999919203264188E-5	9.99959601632094E-5
16	0.0	0.0	0.0	1.999919203264188E-5	9.99959601632094E-5
17	0.0	0.0	0.0	1.999919203264188E-5	9.99959601632094E-5
18	0.0	0.0	0.0	3.999838406528376E-5	9.99959601632094E-5
19	0.0	0.0	0.0	3.999838406528376E-5	9.99959601632094E-5
20	0.0	0.0	0.0	7.999676813056752E-5	1.1999515219585129E-4
21	0.0	0.0	0.0	1.5999353626113504E-4	1.1999515219585129E-4
22	0.0	0.0	0.0	2.9998788048962824E-4	1.1999515219585129E-4
23	0.0	0.0	0.0	3.7998464862019573E-4	1.3999434422849318E-4
24	0.0	0.0	0.0	5.599773769139727E-4	1.3999434422849318E-4
25	0.0	0.0	0.0	6.999717211424658E-4	1.3999434422849318E-4
26	0.0	0.0	0.0	7.999676813056752E-4	1.5999353626113504E-4
27	0.0	0.0	0.0	0.001019958793664736	1.7999272829377692E-4
28	0.0	0.0	0.0	0.001519938594480783	1.7999272829377692E-4
29	0.0	0.0	0.0	0.002639893348308728	1.7999272829377692E-4
30	0.0	0.0	0.0	0.004879802855964619	1.7999272829377692E-4
31	0.0	0.0	0.0	0.013699446542359689	1.7999272829377692E-4
32	0.0	0.0	0.0	0.0242590199355946	1.7999272829377692E-4
33	0.0	0.0	0.0	0.03469859817663366	1.7999272829377692E-4
34	0.0	0.0	0.0	0.04697810208467578	1.7999272829377692E-4
35	0.0	0.0	0.0	0.06181750257289605	1.7999272829377692E-4
36	0.0	0.0	0.0	0.08123671803659133	1.7999272829377692E-4
37	0.0	0.0	0.0	0.11639529762997575	1.7999272829377692E-4
38	0.0	0.0	0.0	0.16119348778309356	1.7999272829377692E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8170	0.0	29.434362	1
GGTATCA	2860	0.0	28.694546	1
TAGGACC	9750	0.0	26.03736	4
CTAGGAC	1495	0.0	22.954868	3
TGTAGGA	27740	0.0	22.934639	2
CTGTAGG	26870	0.0	22.714088	1
AGGACCT	16700	0.0	22.406994	5
GGACCTG	16485	0.0	21.951927	6
GTAGGAC	28565	0.0	21.755827	3
TTAGGAC	16415	0.0	21.28144	3
TAGGACA	6515	0.0	21.103834	4
ACTGTTC	5615	0.0	20.882261	8
GTCCTAC	24960	0.0	20.838161	1
CTTTAGG	16915	0.0	20.616446	1
GATATAC	6330	0.0	20.29858	1
TAGGACG	31130	0.0	20.246094	4
TCCTACA	26150	0.0	20.24063	2
TTTAGGA	17695	0.0	20.189964	2
GGACATG	6540	0.0	20.148598	6
AGGACGT	30995	0.0	20.092964	5
>>END_MODULE