FastQCFastQC Report
Thu 2 Feb 2017
SRR4063907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229912
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38800.17399789767488583No Hit
TATCAACGCAGAGTACTTTTTTTTT25480.11426459878237348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1700.012.294967
GCACCGT550.00306965312.09065156
GTATCAA30150.012.0020261
TAACGAA1900.012.00055413
GTATTAC1302.6266207E-911.6894351
AACGGAC907.4532327E-611.61268915
CGAACGA2000.011.40180516
CCGATAA2000.011.4007819
CGATAAC2000.011.40078110
TCAGGAC3500.011.3959213
AATTCCG2350.011.3181475
GGTATCA12400.011.2593311
AAATCGC1701.0913936E-1111.17698614
GTCTAAT855.3383745E-511.1737251
TAACCTG1451.2205419E-911.1369455
CCGTATG600.005874018711.0840929
CTCGAAT600.00587511.08384413
TAAGACG600.005886788511.0808594
CGGTCGG2000.010.92574810
CGAGCCG3950.010.82425515
Produced by FastQC (version 0.11.5)