##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063907_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2229912
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209828908046596	32.0	32.0	32.0	32.0	32.0
2	30.755426223097594	32.0	32.0	32.0	32.0	32.0
3	30.789287200571145	32.0	32.0	32.0	32.0	32.0
4	30.81981082661558	32.0	32.0	32.0	32.0	32.0
5	30.7003155281464	32.0	32.0	32.0	32.0	32.0
6	34.367921693770874	36.0	36.0	36.0	32.0	36.0
7	34.30802067525535	36.0	36.0	36.0	32.0	36.0
8	34.26208478182099	36.0	36.0	36.0	32.0	36.0
9	34.42520153261653	36.0	36.0	36.0	32.0	36.0
10	34.073736542069824	36.0	36.0	36.0	32.0	36.0
11	34.385586516418584	36.0	36.0	36.0	32.0	36.0
12	34.18493420368158	36.0	36.0	36.0	32.0	36.0
13	34.280834849088215	36.0	36.0	36.0	32.0	36.0
14	34.17454186532922	36.0	36.0	36.0	32.0	36.0
15	34.10126991558411	36.0	36.0	36.0	32.0	36.0
16	34.106349936679116	36.0	36.0	36.0	32.0	36.0
17	34.031301683653886	36.0	36.0	36.0	32.0	36.0
18	34.0161979486186	36.0	36.0	36.0	32.0	36.0
19	34.027263856152175	36.0	36.0	36.0	32.0	36.0
20	34.016886316590075	36.0	36.0	36.0	32.0	36.0
21	34.00447327069409	36.0	36.0	36.0	32.0	36.0
22	33.97533221041907	36.0	36.0	36.0	32.0	36.0
23	33.91851741234632	36.0	36.0	36.0	32.0	36.0
24	33.89664973326302	36.0	36.0	36.0	32.0	36.0
25	33.488373532229076	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	5.0
4	21.0
5	23.0
6	94.0
7	25.0
8	133.0
9	159.0
10	195.0
11	53.0
12	139.0
13	107.0
14	240.0
15	383.0
16	643.0
17	856.0
18	1182.0
19	1697.0
20	2645.0
21	3773.0
22	5904.0
23	9144.0
24	13603.0
25	19639.0
26	27723.0
27	36054.0
28	47959.0
29	64521.0
30	84222.0
31	111953.0
32	155325.0
33	216044.0
34	451676.0
35	973769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.787438835474994	17.525074819573906	11.833313967651385	25.85417237729971
2	16.526257393630164	19.80293220306298	37.84910783118238	25.821702572124472
3	18.66126724876338	24.155488885002267	28.54646234982308	28.63678151641127
4	12.76844119116448	15.823354193032795	35.877726343584996	35.53047827221773
5	14.85471383408463	36.49930057949422	33.39830461725172	15.247680969169435
6	33.86581569953624	35.332164225293994	17.212933145558438	13.589086929611325
7	30.24960516300118	30.275531409557992	21.01371624037129	18.461147187069532
8	28.08752259327	32.98476106649655	19.428260732055595	19.499455608177854
9	27.6133073650292	14.37476641761681	18.559710274843184	39.452215942510804
10	16.419707939128934	26.568654945045083	31.117562465085285	25.894074650740695
11	36.65622556020552	20.982989566521333	23.023066006009806	19.33771886726334
12	25.25616673599491	23.264853183422787	28.821523076432932	22.65745700414937
13	29.5698256462263	19.696614112985554	25.198259827732816	25.535300413055328
14	23.900014270085144	19.76792713102182	24.95972515277517	31.37233344611786
15	25.140783577836828	27.50877457486725	21.974173897670195	25.37626794962573
16	25.75906748074229	25.53501120505749	23.916807047743326	24.789114266456895
17	24.168620473804776	25.889107515856686	25.187845936043757	24.754426074294777
18	24.77915265707941	24.889329496516527	26.280609414801663	24.050908431602398
19	26.00237222371066	24.607804463424625	25.05415590168062	24.335667411184097
20	25.986274332049508	24.367099685221568	24.659752970289475	24.986873012439446
21	26.92951506660216	24.023810015321057	24.070392486489737	24.976282431587045
22	26.215840084946233	24.275109470149665	24.463824025584398	25.045226419319704
23	24.590216908349156	24.337718176228496	25.22541323890437	25.84665167651798
24	25.258006782046426	24.699942016913827	25.015168949788087	25.026882251251664
25	25.15106201171875	24.405894559972428	25.133200252757355	25.30984317555147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	141.0
2	363.0
3	585.0
4	585.0
5	585.0
6	1441.0
7	2297.0
8	2297.0
9	2297.0
10	2538.0
11	2779.0
12	2779.0
13	2779.0
14	3704.5
15	4630.0
16	4630.0
17	4630.0
18	8194.5
19	11759.0
20	11759.0
21	11759.0
22	20449.5
23	29140.0
24	29140.0
25	29140.0
26	44937.5
27	60735.0
28	60735.0
29	60735.0
30	78799.5
31	96864.0
32	96864.0
33	96864.0
34	119585.0
35	142306.0
36	142306.0
37	142306.0
38	163857.0
39	185408.0
40	185408.0
41	185408.0
42	213357.0
43	241306.0
44	241306.0
45	241306.0
46	266603.0
47	291900.0
48	291900.0
49	291900.0
50	297880.0
51	303860.0
52	303860.0
53	303860.0
54	282888.5
55	261917.0
56	261917.0
57	261917.0
58	240146.0
59	218375.0
60	218375.0
61	218375.0
62	190786.0
63	163197.0
64	163197.0
65	163197.0
66	133380.5
67	103564.0
68	103564.0
69	103564.0
70	78469.0
71	53374.0
72	53374.0
73	53374.0
74	40410.5
75	27447.0
76	27447.0
77	27447.0
78	21756.5
79	16066.0
80	16066.0
81	16066.0
82	11036.5
83	6007.0
84	6007.0
85	6007.0
86	4592.5
87	3178.0
88	3178.0
89	3178.0
90	2274.5
91	1371.0
92	1371.0
93	1371.0
94	962.0
95	553.0
96	553.0
97	553.0
98	853.0
99	1153.0
100	1153.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03959797516673304
2	0.005426223097593089
3	0.0018834823975116507
4	0.0042154129849070274
5	0.00937256716856988
6	0.013094687144604808
7	0.022915702503058417
8	0.03672790675147719
9	0.04668345656689591
10	0.05681838565826813
11	0.05475552398480299
12	0.06202038466091935
13	0.06264821212675657
14	0.06610126318886127
15	0.05892609215072164
16	0.0652043668090938
17	0.06085441936722166
18	0.07515991662451253
19	0.07278314121812879
20	0.07524960626248929
21	0.07202077929532645
22	0.07529445108147766
23	0.07991346743728003
24	0.07515991662451253
25	0.07569805445237301
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2229912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.838404466482416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.185444432602	36.71937145191655
2	13.648944207735736	13.331853155157006
3	4.463941493630793	6.54035340541963
4	2.111606044582918	4.1250988031683855
5	1.1676510855218485	2.8513107995221643
6	0.7218954419713804	2.1153732946505017
7	0.470073716813651	1.607035521756546
8	0.3589809357109358	1.4025644907205603
9	0.2734776189305907	1.2020589509276463
>10	1.4095896143608722	12.2947348421528
>50	0.09522185240161607	3.223514830539932
>100	0.0785386943297084	8.125001671918234
>500	0.009940660579096745	3.2379668714750705
>1k	0.004694200829017894	3.2237619106750453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3880	0.17399789767488583	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2548	0.11426459878237348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.345344569651179E-4
2	0.0	0.0	0.0	0.0	1.345344569651179E-4
3	0.0	0.0	0.0	0.0	1.345344569651179E-4
4	0.0	0.0	0.0	0.0	1.345344569651179E-4
5	0.0	0.0	0.0	0.0	1.7937927595349055E-4
6	0.0	0.0	0.0	0.0	2.2422409494186318E-4
7	4.4844818988372636E-5	0.0	0.0	0.0	2.2422409494186318E-4
8	4.4844818988372636E-5	0.0	0.0	0.0	2.2422409494186318E-4
9	4.4844818988372636E-5	0.0	0.0	0.0	2.2422409494186318E-4
10	4.4844818988372636E-5	0.0	0.0	0.0	2.690689139302358E-4
11	4.4844818988372636E-5	0.0	0.0	0.0	2.690689139302358E-4
12	4.4844818988372636E-5	0.0	0.0	0.0	3.587585519069811E-4
13	4.4844818988372636E-5	0.0	0.0	0.0	4.036033708953537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	170	0.0	12.29496	7
GCACCGT	55	0.003069653	12.0906515	6
GTATCAA	3015	0.0	12.002026	1
TAACGAA	190	0.0	12.000554	13
GTATTAC	130	2.6266207E-9	11.689435	1
AACGGAC	90	7.4532327E-6	11.612689	15
CGAACGA	200	0.0	11.401805	16
CCGATAA	200	0.0	11.400781	9
CGATAAC	200	0.0	11.400781	10
TCAGGAC	350	0.0	11.395921	3
AATTCCG	235	0.0	11.318147	5
GGTATCA	1240	0.0	11.259331	1
AAATCGC	170	1.0913936E-11	11.176986	14
GTCTAAT	85	5.3383745E-5	11.173725	1
TAACCTG	145	1.2205419E-9	11.136945	5
CCGTATG	60	0.0058740187	11.084092	9
CTCGAAT	60	0.005875	11.083844	13
TAAGACG	60	0.0058867885	11.080859	4
CGGTCGG	200	0.0	10.925748	10
CGAGCCG	395	0.0	10.824255	15
>>END_MODULE