FastQCFastQC Report
Thu 2 Feb 2017
SRR4063907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229912
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33650.024.646991
GGTATCA16500.020.1326351
GATATAC4750.018.062531
AACGCAG48550.016.855136
CGTCTTA2109.094947E-1216.76057215
TCAACGC48950.016.7173984
TTAGGAC6950.016.4580173
ATTTCGT5550.016.25132642
CAACGCA50250.016.1973535
ATCAACG50550.016.144023
TATCAAC51200.016.0679532
GTATAGG2900.015.9304821
GATTTCG5150.015.80563341
TATACTG3350.015.7595885
TAGGACG9600.015.5817444
GTGTATA5400.015.4809451
ACCGTCG5050.015.245978
CGTCGTA4950.015.11058710
ATACACT6000.015.0318024
TAGGACC13250.014.9418744
Produced by FastQC (version 0.11.5)