##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2229912
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32161672747624	32.0	32.0	32.0	32.0	32.0
2	31.377132371142896	32.0	32.0	32.0	32.0	32.0
3	31.465501777648626	32.0	32.0	32.0	32.0	32.0
4	31.558536390673712	32.0	32.0	32.0	32.0	32.0
5	31.5034001341757	32.0	32.0	32.0	32.0	32.0
6	35.0828647049749	36.0	36.0	36.0	36.0	36.0
7	35.102834551318615	36.0	36.0	36.0	36.0	36.0
8	35.04393267537015	36.0	36.0	36.0	36.0	36.0
9	35.1594614495998	36.0	36.0	36.0	36.0	36.0
10	35.007159923799684	36.0	36.0	36.0	36.0	36.0
11	35.159510330452505	36.0	36.0	36.0	36.0	36.0
12	35.067400866043144	36.0	36.0	36.0	36.0	36.0
13	35.104699198892156	36.0	36.0	36.0	36.0	36.0
14	35.04816243869713	36.0	36.0	36.0	36.0	36.0
15	35.013475419657816	36.0	36.0	36.0	36.0	36.0
16	35.0317752449424	36.0	36.0	36.0	36.0	36.0
17	35.0014538690316	36.0	36.0	36.0	36.0	36.0
18	34.995199810575485	36.0	36.0	36.0	36.0	36.0
19	34.99266249071712	36.0	36.0	36.0	36.0	36.0
20	34.98102167260412	36.0	36.0	36.0	36.0	36.0
21	34.98434646748392	36.0	36.0	36.0	36.0	36.0
22	34.96065808874969	36.0	36.0	36.0	36.0	36.0
23	34.91017044618801	36.0	36.0	36.0	32.0	36.0
24	34.88384653744183	36.0	36.0	36.0	32.0	36.0
25	34.85649613078902	36.0	36.0	36.0	32.0	36.0
26	34.796635472610575	36.0	36.0	36.0	32.0	36.0
27	34.78636511216586	36.0	36.0	36.0	32.0	36.0
28	34.7618004656686	36.0	36.0	36.0	32.0	36.0
29	34.73908432261004	36.0	36.0	36.0	32.0	36.0
30	34.71543092283462	36.0	36.0	36.0	32.0	36.0
31	34.71171328734049	36.0	36.0	36.0	32.0	36.0
32	34.668323682728285	36.0	36.0	36.0	32.0	36.0
33	34.65074451368484	36.0	36.0	36.0	32.0	36.0
34	34.636364125579846	36.0	36.0	36.0	32.0	36.0
35	34.610261750239474	36.0	36.0	36.0	32.0	36.0
36	34.588042487775304	36.0	36.0	36.0	32.0	36.0
37	34.56798564248275	36.0	36.0	36.0	32.0	36.0
38	34.52611672568245	36.0	36.0	36.0	32.0	36.0
39	34.523593756166164	36.0	36.0	36.0	32.0	36.0
40	34.51136726471717	36.0	36.0	36.0	32.0	36.0
41	34.49302663064731	36.0	36.0	36.0	32.0	36.0
42	34.43057752951686	36.0	36.0	36.0	32.0	36.0
43	34.426274220686736	36.0	36.0	36.0	32.0	36.0
44	34.36382108352258	36.0	36.0	36.0	32.0	36.0
45	34.333834698409625	36.0	36.0	36.0	32.0	36.0
46	34.30926870656779	36.0	36.0	36.0	32.0	36.0
47	34.28996794492339	36.0	36.0	36.0	32.0	36.0
48	34.244889932876276	36.0	36.0	36.0	32.0	36.0
49	34.23386662792074	36.0	36.0	36.0	32.0	36.0
50	33.62475873487384	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	5.0
20	24.0
21	71.0
22	222.0
23	698.0
24	1784.0
25	4103.0
26	8334.0
27	15298.0
28	24932.0
29	38680.0
30	55940.0
31	79872.0
32	117151.0
33	189712.0
34	396844.0
35	1296237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95449652871213	18.41042821520191	12.374333997093807	26.26074125899216
2	15.83213783930318	20.55795302123278	37.24685535366817	26.36305378579587
3	17.90999261852293	24.79830988391439	28.855252194487125	28.43644530307556
4	12.478878090256476	16.51029278285421	35.90531823677347	35.105510890115845
5	14.513083924388049	36.945807726941695	33.119602926034744	15.421505422635512
6	33.93611019677098	35.690492227909566	16.986890006825412	13.386507568494041
7	30.179079056589302	30.385589725858768	20.96767177494687	18.467659442605058
8	28.088642063005175	33.18897786100976	19.208291627651676	19.514088448333386
9	27.64989358181285	14.162049830439457	18.427880937197575	39.76017565055012
10	16.299257408315107	26.711416197996947	31.166697236145414	25.822629157542536
11	36.95338650135073	20.885667237092765	22.93216055162715	19.22878570992936
12	25.179547685578203	23.324673787136604	28.828889430017103	22.666889097268093
13	29.675421295785565	19.655761274242053	25.202228255361437	25.46658917461095
14	23.891141599690748	19.672622971551267	24.961904223765487	31.474331204992495
15	25.16804137396583	27.596277347646076	21.901616641432806	25.33406463695529
16	25.78149454637754	25.603728248966096	23.79090665296198	24.823870551694384
17	24.199385446600584	26.001833256200246	25.037266044579336	24.761515252619834
18	24.881508405066583	24.859624064702306	26.23327002719847	24.02559750303264
19	26.013280350650763	24.51541787990642	25.084812801945905	24.38648896749691
20	26.218440288802185	24.287546526750077	24.64258486927665	24.851428315171084
21	27.02550593924783	24.000588364025127	24.059245387261917	24.914660309465127
22	26.239680632707437	24.33027536101249	24.535261304385067	24.89478270189501
23	24.760156653256455	24.24775538557231	25.19708344750174	25.79500451366949
24	25.402506477863152	24.671846565454448	24.964325786514387	24.961321170168016
25	25.149593721256768	24.326676900433345	25.22399257715574	25.29973680115415
26	24.877263841369317	25.127243323677682	25.335514711521256	24.659978123431742
27	25.410710217436932	24.917929711955715	24.56955703571089	25.101803034896463
28	24.843889864546092	24.588400223155425	25.225980744772265	25.34172916752622
29	25.079792326931262	24.54060831867241	24.93682313302548	25.442776221370845
30	24.750036662037008	24.705190133584352	25.375286961723937	25.1694862426547
31	25.71039290468178	24.31938619182761	24.486131193065415	25.484089710425195
32	25.17297081067118	24.826177116369845	24.29578412238279	25.705067950576183
33	24.737377433833103	24.530813304774924	25.064938093546736	25.66687116784524
34	25.428963639274553	24.45118530341265	25.258010512810046	24.86184054450275
35	25.783785553384508	24.477196009957616	25.094995136446336	24.64402330021154
36	24.85696923218366	24.906256937390264	24.859525555383637	25.377248275042437
37	25.80381291760265	24.544889075050644	24.543050378928363	25.10824762841835
38	25.193350838993524	24.694686187495936	24.89331787573074	25.218645097779802
39	25.75078482375101	24.35429186474123	24.4563637994439	25.43855951206386
40	26.08361646174986	24.453191428294662	24.84281439811144	24.620377711844036
41	25.03005843961606	24.747031490840303	25.3413835986085	24.881526470935135
42	26.011483185783558	25.09438895289364	24.750960706660347	24.14316715466245
43	25.24928505025165	24.353092121224623	25.064566341551576	25.33305648697215
44	24.905297721898414	24.87009408116928	24.689053192476916	25.53555500445539
45	24.98904648103909	25.09456799635137	24.651673612490736	25.26471191011881
46	24.974841830904815	24.65675467690812	24.85196592480712	25.516437567379946
47	25.480601705666857	24.540229189173676	25.00873372824584	24.970435376913628
48	25.757128660299784	25.095610869144057	24.215784583150963	24.931475887405195
49	25.27833011795116	25.04500189694095	24.48816228128002	25.188505703827875
50	25.208105928855172	25.381613490268958	24.234059861678283	25.17622071919759
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	29.5
2	29.0
3	105.0
4	181.0
5	222.5
6	264.0
7	253.5
8	243.0
9	334.0
10	425.0
11	600.0
12	775.0
13	1514.5
14	2254.0
15	2982.5
16	3711.0
17	3804.5
18	3898.0
19	3898.5
20	3899.0
21	4411.5
22	4924.0
23	5847.0
24	6770.0
25	8524.0
26	10278.0
27	13754.0
28	17230.0
29	21197.5
30	25165.0
31	29566.5
32	33968.0
33	41010.0
34	48052.0
35	57054.0
36	66056.0
37	75960.0
38	85864.0
39	92333.5
40	98803.0
41	106444.5
42	114086.0
43	116897.0
44	119708.0
45	131816.0
46	143924.0
47	154437.5
48	164951.0
49	170190.0
50	175429.0
51	171404.0
52	167379.0
53	165616.5
54	163854.0
55	163713.5
56	163573.0
57	159914.0
58	156255.0
59	141670.5
60	127086.0
61	111701.0
62	96316.0
63	83481.5
64	70647.0
65	59945.0
66	49243.0
67	42092.0
68	34941.0
69	31118.0
70	27295.0
71	21142.0
72	14989.0
73	12643.0
74	10297.0
75	8198.0
76	6099.0
77	5248.0
78	4397.0
79	3598.0
80	2799.0
81	2252.0
82	1705.0
83	1409.5
84	1114.0
85	794.0
86	474.0
87	326.0
88	178.0
89	138.5
90	99.0
91	92.5
92	86.0
93	72.0
94	58.0
95	58.5
96	59.0
97	43.5
98	28.0
99	26.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008610205245767546
2	4.93293008872099E-4
3	2.690689139302358E-4
4	0.0
5	0.0
6	4.4844818988372636E-4
7	1.345344569651179E-4
8	0.0
9	0.0013453445696511791
10	8.5205156077908E-4
11	0.0
12	1.345344569651179E-4
13	2.690689139302358E-4
14	2.690689139302358E-4
15	0.004663861174790754
16	8.072067417907074E-4
17	0.0
18	3.139137329186085E-4
19	4.4844818988372636E-5
20	5.381378278604716E-4
21	0.0
22	1.345344569651179E-4
23	8.5205156077908E-4
24	4.4844818988372636E-4
25	0.0017489479405465328
26	0.00578498164950007
27	0.005201999002651226
28	0.00340820624311632
29	0.0027355339582907307
30	0.0038118096140116737
31	0.007175171038139622
32	0.002511309863348868
33	0.004305102622883773
34	0.0027355339582907307
35	0.0027355339582907307
36	0.006592188391290777
37	0.003183982148174457
38	0.007040636581174504
39	0.005022619726697736
40	0.0028700684152558487
41	0.003991188889965165
42	0.0019283272165000235
43	0.0015695686645930424
44	0.0013004997506628065
45	0.0015695686645930424
46	8.968963797674527E-4
47	0.002287085768407004
48	0.0017937927595349055
49	2.690689139302358E-4
50	0.00170410312155816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2229912.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.94193504919984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97683354814053	39.46383753401383
2	13.382695441800236	13.902461948424211
3	4.460403394193198	6.95045950183241
4	1.9681058631575266	4.089089076563105
5	1.0939406901279132	2.8410698137150456
6	0.6672939810120683	2.0796324372270503
7	0.46288434699978465	1.6830176081007866
8	0.33706669102267917	1.400631693787897
9	0.2387349374395433	1.1160319153013591
>10	1.25496822451457	11.519602524259076
>50	0.08175461962218479	2.9481143403310264
>100	0.06401581316798217	6.945002303279328
>500	0.008433365644554963	2.992797953255919
>1k	0.00286908315742587	2.068251349908954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.345344569651179E-4
7	0.0	0.0	0.0	0.0	1.345344569651179E-4
8	0.0	0.0	0.0	0.0	1.345344569651179E-4
9	0.0	0.0	0.0	0.0	1.345344569651179E-4
10	0.0	0.0	0.0	0.0	1.345344569651179E-4
11	0.0	0.0	0.0	0.0	1.345344569651179E-4
12	0.0	0.0	0.0	0.0	2.690689139302358E-4
13	0.0	0.0	0.0	0.0	2.690689139302358E-4
14	4.4844818988372636E-5	0.0	0.0	0.0	3.1391373291860843E-4
15	4.4844818988372636E-5	0.0	0.0	0.0	4.93293008872099E-4
16	4.4844818988372636E-5	0.0	0.0	0.0	6.278274658372169E-4
17	4.4844818988372636E-5	0.0	0.0	0.0	6.278274658372169E-4
18	4.4844818988372636E-5	0.0	0.0	4.4844818988372636E-5	6.278274658372169E-4
19	4.4844818988372636E-5	0.0	0.0	8.968963797674527E-5	6.278274658372169E-4
20	4.4844818988372636E-5	0.0	0.0	1.7937927595349055E-4	6.278274658372169E-4
21	4.4844818988372636E-5	0.0	0.0	2.2422409494186318E-4	6.278274658372169E-4
22	4.4844818988372636E-5	0.0	0.0	3.1391373291860843E-4	6.278274658372169E-4
23	4.4844818988372636E-5	0.0	0.0	4.036033708953537E-4	7.623619228023348E-4
24	4.4844818988372636E-5	0.0	0.0	7.175171038139622E-4	7.623619228023348E-4
25	4.4844818988372636E-5	0.0	0.0	8.968963797674527E-4	7.623619228023348E-4
26	4.4844818988372636E-5	0.0	0.0	0.0013004997506628063	8.520515607790801E-4
27	4.4844818988372636E-5	0.0	0.0	0.0016592583025697874	8.520515607790801E-4
28	4.4844818988372636E-5	0.0	0.0	0.0027803787772791035	8.520515607790801E-4
29	8.968963797674527E-5	0.0	0.0	0.004619016355802382	8.520515607790801E-4
30	8.968963797674527E-5	0.0	0.0	0.008296291512848938	8.520515607790801E-4
31	8.968963797674527E-5	0.0	0.0	0.02009047890679094	8.968963797674527E-4
32	8.968963797674527E-5	0.0	0.0	0.03251249376657016	9.86586017744198E-4
33	8.968963797674527E-5	0.0	0.0	0.04488966380736101	9.86586017744198E-4
34	8.968963797674527E-5	0.0	0.0	0.05937454034060537	0.0011211204747093159
35	8.968963797674527E-5	0.0	0.0	0.0773573127549428	0.0011211204747093159
36	8.968963797674527E-5	0.0	0.0	0.10287401475932682	0.0011211204747093159
37	8.968963797674527E-5	0.0	0.0	0.13816688730317608	0.0011211204747093159
38	8.968963797674527E-5	0.0	0.0	0.18485034387007201	0.0011659652936976885
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3365	0.0	24.64699	1
GGTATCA	1650	0.0	20.132635	1
GATATAC	475	0.0	18.06253	1
AACGCAG	4855	0.0	16.85513	6
CGTCTTA	210	9.094947E-12	16.760572	15
TCAACGC	4895	0.0	16.717398	4
TTAGGAC	695	0.0	16.458017	3
ATTTCGT	555	0.0	16.251326	42
CAACGCA	5025	0.0	16.197353	5
ATCAACG	5055	0.0	16.14402	3
TATCAAC	5120	0.0	16.067953	2
GTATAGG	290	0.0	15.930482	1
GATTTCG	515	0.0	15.805633	41
TATACTG	335	0.0	15.759588	5
TAGGACG	960	0.0	15.581744	4
GTGTATA	540	0.0	15.480945	1
ACCGTCG	505	0.0	15.24597	8
CGTCGTA	495	0.0	15.110587	10
ATACACT	600	0.0	15.031802	4
TAGGACC	1325	0.0	14.941874	4
>>END_MODULE