##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063906_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13507
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3048049159695	32.0	32.0	32.0	32.0	32.0
2	30.949137484267418	32.0	32.0	32.0	32.0	32.0
3	31.096690604871547	32.0	32.0	32.0	32.0	32.0
4	30.932035240986156	32.0	32.0	32.0	32.0	32.0
5	31.050196194565782	32.0	32.0	32.0	32.0	32.0
6	34.62952543125787	36.0	36.0	36.0	32.0	36.0
7	34.568964240764046	36.0	36.0	36.0	32.0	36.0
8	34.64736803139113	36.0	36.0	36.0	32.0	36.0
9	34.672984378470424	36.0	36.0	36.0	32.0	36.0
10	34.45058118012882	36.0	36.0	36.0	32.0	36.0
11	34.602280299104166	36.0	36.0	36.0	32.0	36.0
12	34.57503516695047	36.0	36.0	36.0	32.0	36.0
13	34.54978899829718	36.0	36.0	36.0	32.0	36.0
14	34.50795883615903	36.0	36.0	36.0	32.0	36.0
15	34.42363219071593	36.0	36.0	36.0	32.0	36.0
16	34.450729251499226	36.0	36.0	36.0	32.0	36.0
17	34.34974457688606	36.0	36.0	36.0	32.0	36.0
18	34.416006515140296	36.0	36.0	36.0	32.0	36.0
19	34.395572666025025	36.0	36.0	36.0	32.0	36.0
20	34.4448063966832	36.0	36.0	36.0	32.0	36.0
21	34.379432886651365	36.0	36.0	36.0	32.0	36.0
22	34.375731102391356	36.0	36.0	36.0	32.0	36.0
23	34.31287480565633	36.0	36.0	36.0	32.0	36.0
24	34.22906641000962	36.0	36.0	36.0	32.0	36.0
25	34.045605982083366	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	0.0
7	0.0
8	1.0
9	1.0
10	3.0
11	0.0
12	1.0
13	2.0
14	2.0
15	1.0
16	4.0
17	6.0
18	7.0
19	13.0
20	14.0
21	17.0
22	28.0
23	39.0
24	54.0
25	91.0
26	109.0
27	173.0
28	229.0
29	296.0
30	413.0
31	560.0
32	784.0
33	1181.0
34	2553.0
35	6923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77460928820088	18.450485149248202	13.554551514702615	25.22035404784831
2	15.947286592137411	20.078477826312284	40.97875175834753	22.995483823202783
3	19.345524542829644	25.068483008810244	28.888724365143997	26.69726808321611
4	13.951421800947866	16.64691943127962	37.01125592417062	32.3904028436019
5	14.380924170616113	37.040876777251185	34.38240521327014	14.195793838862558
6	31.343504665975413	37.32039697822545	17.612205599170494	13.723892756628647
7	28.037037037037038	30.955555555555552	22.0962962962963	18.91111111111111
8	26.546639994072756	35.20041490701637	19.878491516633325	18.374453582277543
9	27.80617915092243	15.536785952433874	18.441135067051935	38.21589982959176
10	15.747739736179042	28.93878760930784	33.60011857121684	21.71335408329628
11	35.944563847921145	23.575187134069516	22.811828355443563	17.668420662565776
12	22.196694582376047	25.124138442155193	30.80856740532128	21.870599570147483
13	28.59472279869552	22.168692558553214	26.126593536910764	23.1099911058405
14	23.841648750834015	20.40922232930536	25.52450144562236	30.224627474238268
15	24.18111753371869	28.827627093523045	23.039869571661477	23.951385801096784
16	24.608882627715577	27.641432490546453	25.090828204938088	22.658856676799882
17	22.50389148321103	26.5436216737084	27.040249054925507	23.912237788155068
18	22.169496552235486	28.76102913917105	27.596945206495143	21.472529102098317
19	25.192763938315537	26.3567615658363	26.141755634638198	22.308718861209965
20	26.002520198650952	26.52879697576162	24.72759617522793	22.7410866503595
21	24.36600919472045	26.39774581047012	25.671066290968415	23.56517870384102
22	23.873072360616845	25.904507710557535	26.67556346381969	23.54685646500593
23	24.601586242680305	26.32125120450671	25.646727447928246	23.43043510488474
24	24.13997627520759	25.467081850533805	26.445729537366546	23.94721233689205
25	23.750926612305413	26.523350630096367	26.360266864343956	23.36545589325426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	4.0
7	5.0
8	5.0
9	5.0
10	12.0
11	19.0
12	19.0
13	19.0
14	35.5
15	52.0
16	52.0
17	52.0
18	93.5
19	135.0
20	135.0
21	135.0
22	243.0
23	351.0
24	351.0
25	351.0
26	514.5
27	678.0
28	678.0
29	678.0
30	799.0
31	920.0
32	920.0
33	920.0
34	979.0
35	1038.0
36	1038.0
37	1038.0
38	1162.5
39	1287.0
40	1287.0
41	1287.0
42	1401.0
43	1515.0
44	1515.0
45	1515.0
46	1657.0
47	1799.0
48	1799.0
49	1799.0
50	1717.0
51	1635.0
52	1635.0
53	1635.0
54	1490.5
55	1346.0
56	1346.0
57	1346.0
58	1135.0
59	924.0
60	924.0
61	924.0
62	822.0
63	720.0
64	720.0
65	720.0
66	622.5
67	525.0
68	525.0
69	525.0
70	415.0
71	305.0
72	305.0
73	305.0
74	204.5
75	104.0
76	104.0
77	104.0
78	75.5
79	47.0
80	47.0
81	47.0
82	29.5
83	12.0
84	12.0
85	12.0
86	6.5
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	2.0
98	42.5
99	83.0
100	83.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04442141112015992
2	0.0
3	0.0
4	0.02221070556007996
5	0.02221070556007996
6	0.037017842600133265
7	0.05182497964018657
8	0.07403568520026653
9	0.07403568520026653
10	0.0962463907603465
11	0.10364995928037314
12	0.10364995928037314
13	0.1110535278003998
14	0.13326423336047974
15	0.0962463907603465
16	0.14807137040053306
17	0.11845709632042645
18	0.14807137040053306
19	0.1406678018805064
20	0.11845709632042645
21	0.1554749389205597
22	0.1406678018805064
23	0.11845709632042645
24	0.1406678018805064
25	0.1258606648404531
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	13507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.587547197749316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.87357454596649	34.64129710520471
2	14.979586090384345	15.754793810616718
3	7.77136421230466	12.260309469164136
4	3.9842320146417003	8.380839564670172
5	2.5763761790792623	6.774265195824388
6	1.506405744051809	4.753090989857111
7	0.9010277347599606	3.3167986969719405
8	0.7461635928480923	3.1391130524913007
9	0.38012107560185837	1.7990671503664766
>10	1.2670702520061945	8.58073591471089
>50	0.014078558355624384	0.5996890501221589
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	81	0.5996890501221589	No Hit
GATATACACTGTTCTACAAATCCCG	26	0.192492781520693	No Hit
GTATCAACGCAGAGTACATGGGGGC	25	0.18508921300066633	No Hit
ATATTAACATGTATAGCAATTGAGG	21	0.1554749389205597	No Hit
GTCTAAACTCCATCCCCACAGTTCC	20	0.14807137040053306	No Hit
TTCCTAGACAGAGGCATAATTCAGC	20	0.14807137040053306	No Hit
GTATCAACGCAGAGTACTTTTTTTT	19	0.1406678018805064	No Hit
ATCATACACAGCGTGACAGGCACGA	19	0.1406678018805064	No Hit
TATCAACGCAGAGTACATGGGGGCC	17	0.1258606648404531	No Hit
GGTATCAACGCAGAGTACATGGGGG	17	0.1258606648404531	No Hit
ACATAAGAGGGATACCTGCACCAAT	17	0.1258606648404531	No Hit
GTGTACTACTGTGGACTAAAGTACA	17	0.1258606648404531	No Hit
TTATAATACAACGTAAATAAATGTC	17	0.1258606648404531	No Hit
GTTCTACAAATCCCGTTTCCAACGA	17	0.1258606648404531	No Hit
GGGTGGCCCAGGTCCGTTTTGTGAC	16	0.11845709632042645	No Hit
TTATTAAGCCACTTCCCAGCAGTCA	16	0.11845709632042645	No Hit
GTCTAGGAATGAAGAGATGATATTC	15	0.1110535278003998	No Hit
GTCCTACCTTATCCATGAGCACATG	15	0.1110535278003998	No Hit
GGTATCAACGCAGAGTACTTTTTTT	15	0.1110535278003998	No Hit
TCCTTATCCTTTCTGTTCCTCTCAA	15	0.1110535278003998	No Hit
GTGATAATCTGTTATAAAAACAGAA	15	0.1110535278003998	No Hit
CTCCCGGGAAGGGCCTGTCCCAGTC	15	0.1110535278003998	No Hit
GTCTTATAGTATCGAGCCAATCGGT	14	0.10364995928037314	No Hit
CCTGTGGAGTATGCAAAAGTGTGGA	14	0.10364995928037314	No Hit
GTGTAAAACTATACACCTGCATCCA	14	0.10364995928037314	No Hit
GATTAGATGAGTGAGTTACACTGAA	14	0.10364995928037314	No Hit
ACAATATTCTGTGCCCTTGGAAATC	14	0.10364995928037314	No Hit
GCACTAGAGTGTAAAACTATACACC	14	0.10364995928037314	No Hit
GTACATGGGGGCCCAAAAGAAGGTC	14	0.10364995928037314	No Hit
GTACATGGGGTGTGATGAGCGGACG	14	0.10364995928037314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE