##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13507
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362182571999703	32.0	32.0	32.0	32.0	32.0
2	31.471015029244096	32.0	32.0	32.0	32.0	32.0
3	31.513067298437846	32.0	32.0	32.0	32.0	32.0
4	31.58036573628489	32.0	32.0	32.0	32.0	32.0
5	31.573110239135264	32.0	32.0	32.0	32.0	32.0
6	35.19012363959428	36.0	36.0	36.0	36.0	36.0
7	35.18686606944547	36.0	36.0	36.0	36.0	36.0
8	35.079218183164286	36.0	36.0	36.0	36.0	36.0
9	35.19116013918709	36.0	36.0	36.0	36.0	36.0
10	35.1093507070408	36.0	36.0	36.0	36.0	36.0
11	35.15488265343896	36.0	36.0	36.0	36.0	36.0
12	35.079736432960686	36.0	36.0	36.0	36.0	36.0
13	35.162212186273784	36.0	36.0	36.0	36.0	36.0
14	35.06826090175465	36.0	36.0	36.0	36.0	36.0
15	35.09321092766714	36.0	36.0	36.0	36.0	36.0
16	35.090397571629524	36.0	36.0	36.0	36.0	36.0
17	35.055082549789	36.0	36.0	36.0	36.0	36.0
18	35.021100170282075	36.0	36.0	36.0	36.0	36.0
19	35.051750943954985	36.0	36.0	36.0	36.0	36.0
20	35.065447545717035	36.0	36.0	36.0	36.0	36.0
21	35.0542681572518	36.0	36.0	36.0	36.0	36.0
22	35.04294069741616	36.0	36.0	36.0	36.0	36.0
23	34.99163396757237	36.0	36.0	36.0	36.0	36.0
24	34.96039090841786	36.0	36.0	36.0	36.0	36.0
25	34.987265862145556	36.0	36.0	36.0	36.0	36.0
26	34.89220404234841	36.0	36.0	36.0	32.0	36.0
27	34.86903087288073	36.0	36.0	36.0	32.0	36.0
28	34.926482564596135	36.0	36.0	36.0	36.0	36.0
29	34.832901458502995	36.0	36.0	36.0	32.0	36.0
30	34.84215591915303	36.0	36.0	36.0	32.0	36.0
31	34.833271636929	36.0	36.0	36.0	32.0	36.0
32	34.73280521211224	36.0	36.0	36.0	32.0	36.0
33	34.818908714000145	36.0	36.0	36.0	32.0	36.0
34	34.759309987413936	36.0	36.0	36.0	32.0	36.0
35	34.76449248537795	36.0	36.0	36.0	32.0	36.0
36	34.76634337750796	36.0	36.0	36.0	32.0	36.0
37	34.71281557710817	36.0	36.0	36.0	32.0	36.0
38	34.63315317983268	36.0	36.0	36.0	32.0	36.0
39	34.68357148145406	36.0	36.0	36.0	32.0	36.0
40	34.69008662175168	36.0	36.0	36.0	32.0	36.0
41	34.61205300955061	36.0	36.0	36.0	32.0	36.0
42	34.51780558229066	36.0	36.0	36.0	32.0	36.0
43	34.565188420818835	36.0	36.0	36.0	32.0	36.0
44	34.534241504405124	36.0	36.0	36.0	32.0	36.0
45	34.47604945583771	36.0	36.0	36.0	32.0	36.0
46	34.54060857333235	36.0	36.0	36.0	32.0	36.0
47	34.42311394091952	36.0	36.0	36.0	32.0	36.0
48	34.39868216480343	36.0	36.0	36.0	32.0	36.0
49	34.38009920781817	36.0	36.0	36.0	32.0	36.0
50	33.78381579921522	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	3.0
24	12.0
25	36.0
26	50.0
27	110.0
28	139.0
29	230.0
30	323.0
31	437.0
32	600.0
33	957.0
34	1979.0
35	8626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5449429883015	19.798608026062492	14.090034058936768	25.566414926699245
2	15.007033390094026	20.65595617087436	39.964462871103876	24.37254756792774
3	17.590878803583326	25.571925668172057	30.39905234322944	26.43814318501518
4	12.800769971126083	17.16147182942178	37.032649737173315	33.00510846227882
5	13.718812467609387	37.04745687421337	34.13045087732287	15.103279780854372
6	30.539720145109943	38.55778485229881	17.85000370178426	13.05249130080699
7	27.437624935218775	31.65765899163397	21.707262900718145	19.19745317242911
8	26.201228992374325	36.173835788850226	19.37513881690975	18.2497964018657
9	26.948989412897017	15.88065447545717	19.389945953949802	37.78041015769601
10	14.563897527025027	28.964904486894717	34.0515326521546	22.41966533392566
11	35.1669504701266	23.06951950840305	23.299030132523875	18.464499888946474
12	22.29954838232028	24.66869030872881	30.495298733989785	22.53646257496113
13	28.555563781742798	20.974309617235505	27.14148219441771	23.32864440660398
14	23.461908639964463	19.686088694750868	25.660768490412377	31.19123417487229
15	23.12476860422066	29.33728248796742	23.6653091447612	23.87263976305072
16	24.365143999407714	27.541274894499146	25.29799363293107	22.795587473162062
17	23.106537351003183	27.215517879617977	26.09017546457392	23.587769304804915
18	22.44761975272081	28.62219589842304	27.45243207225883	21.47775227659732
19	23.78026208632561	27.348782112978455	26.097579033093954	22.773376767601984
20	24.801954542089288	26.948989412897017	25.223957947730806	23.02509809728289
21	24.639076034648703	26.682460946176057	25.483082845931737	23.195380173243503
22	23.876508477085956	25.993929073813575	26.800918042496484	23.32864440660398
23	24.542829643888354	25.668172058932402	26.01613977937366	23.77285851780558
24	23.550751462204783	26.245650403494487	26.10498260161398	24.098615532686754
25	24.357740430887688	25.919893388613314	26.61582882949582	23.106537351003183
26	24.390966308774527	25.812661977045536	25.760829322473157	24.035542391706773
27	23.152672886124687	27.247149415074784	27.26195764845254	22.338220050347992
28	24.144824522434476	26.728861246853253	24.826003257811344	24.30031097290093
29	23.011994669035985	26.625203613208942	25.38131200947727	24.981489708277802
30	23.72278987116837	26.551162446320152	25.721901377165707	24.00414630534577
31	24.29110831420745	25.764418449692748	25.512697120011847	24.431776116087956
32	23.111670616113745	25.955272511848342	27.073459715639807	23.859597156398106
33	23.920929888206118	26.216036129414377	26.312282520174723	23.550751462204783
34	23.696682464454977	26.081161137440755	26.947571090047397	23.27458530805687
35	23.64699785296513	26.304878951654697	26.216036129414377	23.832087065965794
36	24.087375046279156	26.486486486486488	26.064420584968527	23.36171788226583
37	22.699341082401716	27.37839638705856	27.074850077737466	22.847412452802253
38	24.44839330667851	24.944469124833407	26.099511328298536	24.507626240189545
39	25.364625749611314	25.083290145850302	26.127193307174057	23.424890797364327
40	23.49152291404457	26.0013326423336	26.23084326645443	24.276301177167394
41	22.728955356481826	25.89768268305323	27.98548900570075	23.387872954764198
42	23.174885236191322	26.602991263142307	26.499333629498	23.72278987116837
43	22.995483823202783	25.46087214037166	26.948989412897017	24.59465462352854
44	24.268897608647368	25.231361516250832	26.73428592581624	23.765454949285555
45	21.80350929147849	26.067964759013844	27.000814392537205	25.12771155697046
46	24.439179684607982	25.697786333012512	26.164211149774193	23.698822832605316
47	24.083808395646702	26.03094691641371	26.58621455541571	23.299030132523875
48	22.471494150747816	24.98889382496668	27.43225233229676	25.107359691988744
49	24.083808395646702	24.83897238468942	27.222921448138003	23.854297771525875
50	23.88391204560598	26.164211149774193	25.794032723772858	24.157844080846967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.0
11	3.0
12	3.0
13	5.0
14	7.0
15	15.5
16	24.0
17	24.5
18	25.0
19	26.5
20	28.0
21	42.0
22	56.0
23	62.0
24	68.0
25	104.0
26	140.0
27	179.5
28	219.0
29	275.0
30	331.0
31	384.0
32	437.0
33	468.5
34	500.0
35	582.0
36	664.0
37	783.0
38	902.0
39	865.0
40	828.0
41	775.5
42	723.0
43	735.0
44	747.0
45	740.0
46	733.0
47	778.0
48	823.0
49	913.0
50	1003.0
51	973.0
52	943.0
53	835.0
54	727.0
55	689.5
56	652.0
57	704.5
58	757.0
59	637.0
60	517.0
61	525.0
62	533.0
63	450.0
64	367.0
65	310.0
66	253.0
67	217.5
68	182.0
69	165.5
70	149.0
71	101.0
72	53.0
73	52.0
74	51.0
75	38.0
76	25.0
77	21.5
78	18.0
79	10.5
80	3.0
81	5.5
82	8.0
83	4.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007403568520026653
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.007403568520026653
11	0.0
12	0.0
13	0.0
14	0.0
15	0.014807137040053306
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.014807137040053306
27	0.007403568520026653
28	0.007403568520026653
29	0.007403568520026653
30	0.007403568520026653
31	0.0
32	0.02221070556007996
33	0.0
34	0.02221070556007996
35	0.0
36	0.014807137040053306
37	0.0
38	0.007403568520026653
39	0.0
40	0.0
41	0.0
42	0.007403568520026653
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.007403568520026653
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	13507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.00799585400162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60541969596827	38.42452061893833
2	14.738929279576999	16.509957799659436
3	6.926635822868474	11.638409713481899
4	3.423661599471249	7.6700969867476125
5	2.16787838730998	6.070926186421855
6	1.0971579643093192	3.686977122973273
7	1.0707204230006608	4.197823350855112
8	0.5287508261731658	2.369141926408529
9	0.39656311962987445	1.9989635004071964
>10	1.0442828816920027	7.4331827941067585
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	40	0.2961427408010661	No Hit
CTTTTATACTGGGCCAGGCCTACCTGGATGTTTTCAGGTAACTGTGGGGA	27	0.19989635004071965	No Hit
TTCCTAGACAGAGGCATAATTCAGCCATCCTTTTAACCAGAAAAAAAGTG	23	0.170282075960613	No Hit
GTCTTATAGTATCGAGCCAATCGGTGAATTCTGCTCTCTATCAGAATCAG	21	0.1554749389205597	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	21	0.1554749389205597	No Hit
ATCATACACAGCGTGACAGGCACGAATAGCATGATCACATGCTTCGCCCC	19	0.1406678018805064	No Hit
GTGTACTACTGTGGACTAAAGTACATCAAAGATGACGTCATCCTGAACGA	17	0.1258606648404531	No Hit
GTGTATGATCGTGGTGGTGGCCACTATCAAGTCTGTGCGTTTCTACACTG	16	0.11845709632042645	No Hit
TTATAATACAACGTAAATAAATGTCAACTCCCAACAACTGGCACCATTAT	16	0.11845709632042645	No Hit
GTACATGGGAGAGCAGGTCCAGTTTCACACCAAGACAGGAAAGGCTTCCA	15	0.1110535278003998	No Hit
GTGTAAAACTATACACCTGCATCCAGTGACGCCCCCATAGCTCTCGGAGC	15	0.1110535278003998	No Hit
CCCTAATATCTGCCTTTTTTTTCTTTCTCCAAAAATCATATCCAGCATAT	15	0.1110535278003998	No Hit
GTCTAAACTCCATCCCCACAGTTCCTGACAGCAGCCAGGTATGTTCCTCC	14	0.10364995928037314	No Hit
ACAATATTCTGTGCCCTTGGAAATCAATCATGACAGAAGTCAGTAGAACT	14	0.10364995928037314	No Hit
CTGTAGAACAGATTAGATGGGTGAGTTACACTGAAAAACACATTCGTAGG	14	0.10364995928037314	No Hit
CTATTACTATGTCCTATCATAACATTCCATACATACTTAAAACCAAGTAA	14	0.10364995928037314	No Hit
TTGATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	14	0.10364995928037314	No Hit
GGGTATGCTGTGGCATTGAATTCCTCCCCACGTTTTTTCTTGACTTCTCA	14	0.10364995928037314	No Hit
ATATTAACATGTATAGCAATTGAGGATTCGTAGAGGTCCACTAAAGGAAA	14	0.10364995928037314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.007403568520026653	0.0
16	0.0	0.0	0.0	0.007403568520026653	0.0
17	0.0	0.0	0.0	0.007403568520026653	0.0
18	0.0	0.0	0.0	0.014807137040053306	0.0
19	0.0	0.0	0.0	0.014807137040053306	0.0
20	0.0	0.0	0.0	0.014807137040053306	0.0
21	0.0	0.0	0.0	0.014807137040053306	0.0
22	0.0	0.0	0.0	0.014807137040053306	0.0
23	0.0	0.0	0.0	0.014807137040053306	0.0
24	0.0	0.0	0.0	0.014807137040053306	0.0
25	0.0	0.0	0.0	0.014807137040053306	0.0
26	0.0	0.0	0.0	0.014807137040053306	0.0
27	0.0	0.0	0.0	0.02221070556007996	0.0
28	0.0	0.0	0.0	0.029614274080106612	0.0
29	0.0	0.0	0.0	0.029614274080106612	0.0
30	0.0	0.0	0.0	0.04442141112015992	0.0
31	0.0	0.0	0.0	0.11105352780039979	0.0
32	0.0	0.0	0.0	0.18508921300066633	0.0
33	0.0	0.0	0.0	0.24431776116087955	0.0
34	0.0	0.0	0.0	0.3479677204412527	0.0
35	0.0	0.0	0.0	0.46642481676167913	0.0
36	0.0	0.0	0.0	0.5848819130821056	0.0
37	0.0	0.0	0.0	0.7921818316428518	0.0
38	0.0	0.0	0.0	1.0290960242837048	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE