FastQCFastQC Report
Thu 2 Feb 2017
SRR4063905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921808
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30980.1612023677703496No Hit
CTATTGGAGCTGGAATTACCGCGGC27770.14449934644876075No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27000.14049270270495284No Hit
GTATCTGATCGTCTTCGAACCTCCG26400.13737064264484278No Hit
GAATAGGACCGCGGTTCTATTTTGT25950.13502909759976023No Hit
GATTAAGAGGGACGGCCGGGGGCAT22650.11785776726915488No Hit
TATCAACGCAGAGTACTTTTTTTTT22580.11749352692880871No Hit
GTCCTATTCCATTATTCCTAGCTGC22450.11681708058245152No Hit
GAACTACGACGGTATCTGATCGTCT22260.11582842823008334No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21930.1141112951970228No Hit
GCGCAAGACGGACCAGAGCGAAAGC21890.11390315785968212No Hit
GTACATGGGGAATAATTGCAATCCC21450.11161364714893475No Hit
ATCAGATACCGTCGTAGTTCCGACC20670.10755496907079168No Hit
GAATAACGCCGCCGCATCGCCAGTC19900.10354832532698377No Hit
ATACAGGACTCTTTCGAGGCCCTGT19790.1029759476492969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA250.006039034618.9964851
GTCCTAA1800.012.1366421
GTATCAA26450.011.922181
TTAATAC1603.6379788E-1211.8709483
CGGTCGG3150.011.76279210
GTTCTAG1052.7275019E-711.7597281
CGCCGGT4050.011.7283647
GTAATAC1001.9381077E-611.39611153
GGTATCA9650.011.319151
CGGTCCA4750.011.20084510
GCGGTCG3400.011.1770229
GCCGGTC4600.011.1524328
CGTCTTA1303.2583557E-810.96350815
AGAATTT4450.010.88931317
GTATTAC1408.376446E-910.8551351
GATCTAA700.001493983110.8551351
GCGTTAT1053.47469E-610.8551341
CCGGTCC4400.010.7959879
GGTCGGC3350.010.77580911
ATAGATT1158.842744E-710.7368634
Produced by FastQC (version 0.11.5)