FastQCFastQC Report
Thu 2 Feb 2017
SRR4063905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921808
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA27040.1407008400422935No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24920.12966956116323797No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24700.12852480580786427No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23580.12269696036232547No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23100.12019931231423743No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG22020.11457960420603931No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG21500.11187381882061058No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC21240.11052092612789623No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21040.10948023944119288No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC20750.107971243745473No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20410.10620207637807731No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG19770.10287187898062658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32050.023.406291
ATACCGT6950.020.5742246
TATACCG755.4774788E-520.5320225
TACCGTC6700.020.3569097
ACCGTCG6600.020.3320358
CGTCGTA6650.020.17968810
AATCACG1900.019.68551434
CCGTCGT6850.019.2693429
GGTATCA15550.019.0989551
GTCGTAG7400.017.83716211
TTCCGCG750.00129112317.6002529
TATAGGG1551.441731E-817.031172
AACGCAG44550.016.6902926
TCAACGC45900.016.19944
GTATAGG2051.05501385E-1016.096951
GGCGTTA7800.016.07589742
TATACAG2600.016.0758975
GCATTCG8350.016.0733422
TACGACG6850.016.0573675
TGTAGGA5550.015.8548432
Produced by FastQC (version 0.11.5)