##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1921808
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01747312946975	32.0	32.0	32.0	32.0	32.0
2	30.720583950113642	32.0	32.0	32.0	32.0	32.0
3	31.101610046373	32.0	32.0	32.0	32.0	32.0
4	31.318606229134232	32.0	32.0	32.0	32.0	32.0
5	31.12818866400806	32.0	32.0	32.0	32.0	32.0
6	34.7091015335559	36.0	36.0	36.0	32.0	36.0
7	34.74164224521908	36.0	36.0	36.0	32.0	36.0
8	34.643560647057356	36.0	36.0	36.0	32.0	36.0
9	34.88594438154072	36.0	36.0	36.0	32.0	36.0
10	34.56403085011614	36.0	36.0	36.0	32.0	36.0
11	34.90335819186932	36.0	36.0	36.0	32.0	36.0
12	34.72442564501761	36.0	36.0	36.0	32.0	36.0
13	34.830683918476765	36.0	36.0	36.0	32.0	36.0
14	34.74351339988178	36.0	36.0	36.0	32.0	36.0
15	34.66416312139402	36.0	36.0	36.0	32.0	36.0
16	34.69531711804717	36.0	36.0	36.0	32.0	36.0
17	34.63950040794918	36.0	36.0	36.0	32.0	36.0
18	34.65279153796841	36.0	36.0	36.0	32.0	36.0
19	34.65761303938791	36.0	36.0	36.0	32.0	36.0
20	34.65635588987037	36.0	36.0	36.0	32.0	36.0
21	34.6446689783787	36.0	36.0	36.0	32.0	36.0
22	34.63011237334843	36.0	36.0	36.0	32.0	36.0
23	34.55628033601692	36.0	36.0	36.0	32.0	36.0
24	34.52621697901143	36.0	36.0	36.0	32.0	36.0
25	34.48448284115791	36.0	36.0	36.0	32.0	36.0
26	34.399907274816215	36.0	36.0	36.0	32.0	36.0
27	34.39931356306145	36.0	36.0	36.0	32.0	36.0
28	34.374454680176164	36.0	36.0	36.0	32.0	36.0
29	34.331787046364674	36.0	36.0	36.0	32.0	36.0
30	34.309764034700656	36.0	36.0	36.0	32.0	36.0
31	34.31816133557567	36.0	36.0	36.0	32.0	36.0
32	34.265831446221476	36.0	36.0	36.0	32.0	36.0
33	34.223376632837414	36.0	36.0	36.0	32.0	36.0
34	34.22021086393646	36.0	36.0	36.0	32.0	36.0
35	34.180013299975855	36.0	36.0	36.0	32.0	36.0
36	34.135481796308476	36.0	36.0	36.0	32.0	36.0
37	34.13752830667788	36.0	36.0	36.0	32.0	36.0
38	34.083569742658995	36.0	36.0	36.0	32.0	36.0
39	34.096008550281816	36.0	36.0	36.0	32.0	36.0
40	34.06654983224131	36.0	36.0	36.0	32.0	36.0
41	34.03744234595756	36.0	36.0	36.0	32.0	36.0
42	33.952351639705945	36.0	36.0	36.0	32.0	36.0
43	33.96158044924363	36.0	36.0	36.0	32.0	36.0
44	33.86446044558041	36.0	36.0	36.0	32.0	36.0
45	33.83719185267207	36.0	36.0	36.0	32.0	36.0
46	33.82332574325843	36.0	36.0	36.0	32.0	36.0
47	33.8135021812793	36.0	36.0	36.0	32.0	36.0
48	33.75643872853063	36.0	36.0	36.0	32.0	36.0
49	33.73130770607678	36.0	36.0	36.0	27.0	36.0
50	32.953621277463725	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	3.0
20	11.0
21	48.0
22	204.0
23	545.0
24	1664.0
25	3707.0
26	7907.0
27	14846.0
28	25840.0
29	41903.0
30	64094.0
31	98081.0
32	151531.0
33	251382.0
34	465962.0
35	794076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13917512361489	19.084724206871645	12.766184418539058	26.009916250974403
2	16.244631329619448	20.689011019888667	36.25578754895156	26.810570101540325
3	17.81512692494816	24.57705126170449	28.913079110523697	28.69474270282365
4	12.399105425724109	16.44898970136455	36.0977787583359	35.05412611457544
5	15.228472355198855	36.18665340138036	32.51646366338365	16.06841058003713
6	34.58989574379073	35.97801857005189	16.235578654222806	13.19650703193458
7	30.65755302171342	30.429746587456492	20.524329446193086	18.388370944637
8	28.550510769025834	32.30239441192877	18.889920325027266	20.25717449401813
9	27.236894071807278	14.137236097936135	18.24540023509418	40.38046959516241
10	16.039673408815005	26.172825244784683	30.6567155325423	27.13078581385801
11	37.52024135605638	20.834859673807166	22.24587471797391	19.399024252162548
12	25.208384825723734	23.238830162269323	28.06564661867359	23.48713839333335
13	29.239380539722614	19.10441346194873	25.37186453130968	26.284341467018972
14	24.03512533015854	19.634208275141482	24.598762412816292	31.73190398188369
15	25.412921995846453	27.105926896116056	21.772141886663636	25.709009221373858
16	26.477922099546987	25.527139858444176	22.978260846941335	25.016677195067505
17	24.380219043733817	25.850084920033634	24.513687111303522	25.256008924929024
18	25.444191557316397	24.42452568053175	25.672986763492254	24.458295998659594
19	25.937866842057062	24.750027057853856	24.86434649038822	24.447759609700864
20	26.23378272269698	23.838415479710875	24.24241320475202	25.685388592840123
21	27.419284340579285	23.871791562944892	23.942558257640723	24.766365838835096
22	26.232564577277834	23.84507073034427	24.52562849736134	25.39673619501656
23	24.563430117598084	23.586793630970966	25.321989801228018	26.527786450202935
24	25.220339618046772	24.682408109871652	24.631570512320184	25.465681759761395
25	25.235445172066594	24.13745554263354	24.90793715217883	25.71916213312103
26	25.15011284156233	24.921151405432944	25.19538476188791	24.73335099111682
27	25.838921285074523	24.41611411598309	24.2177995039793	25.527165094963088
28	24.814737916790904	24.263257667412347	25.369496450346745	25.552507965450005
29	24.97542618462962	24.32409649274806	25.225665418332454	25.47481190428987
30	24.907649518278866	24.431001870685908	25.482124412321028	25.179224198714195
31	25.628848833784502	24.297986247493085	24.324005066384412	25.749159852338
32	25.320929438713012	24.326789685715504	24.120260469298486	26.232020406272994
33	24.686925327280505	24.116350443815154	25.001847278464478	26.19487695043986
34	25.619118684708425	24.069917013743623	25.16391190593642	25.14705239561153
35	26.408754854904444	23.91048243076167	24.777808767759687	24.902953946574197
36	24.96177827548991	24.678016446009277	24.534652455505128	25.82555282299569
37	26.15121873546244	24.400206895188024	24.382774830804905	25.065799538544635
38	25.176200725201802	24.095631304405746	25.03960093085605	25.6885670395364
39	25.770457817808484	24.106138841377142	24.281239934288955	25.842163406525415
40	26.008924041576147	24.456505053921504	24.77964381886537	24.754927085636975
41	24.786627660714824	24.864889335702	25.17382521628634	25.174657787296837
42	26.51196673925543	24.870888262734905	24.309379040839634	24.307765957170027
43	25.5288957621214	23.788536398674776	24.8644620865892	25.818105752614624
44	25.233713915227124	24.507309316191016	24.33684359509916	25.922133173482692
45	25.317815847629475	24.806365318981427	24.47927425955711	25.396544573831985
46	25.16085222409258	24.570570742456923	24.573848927695202	25.6947281057553
47	25.54532890409704	24.271715329986428	24.964095711466893	25.21886005444963
48	26.444143681453486	25.061726660557742	23.694920175524864	24.799209482463905
49	25.299367935980815	25.097369704115778	24.07905301230774	25.524209347595665
50	25.620750697528237	24.959022492618846	23.96583178355967	25.454395026293252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	16.5
2	24.0
3	62.0
4	100.0
5	122.5
6	145.0
7	146.0
8	147.0
9	220.0
10	293.0
11	438.5
12	584.0
13	1173.5
14	1763.0
15	2401.5
16	3040.0
17	3377.5
18	3715.0
19	3824.0
20	3933.0
21	4432.5
22	4932.0
23	5657.5
24	6383.0
25	7986.0
26	9589.0
27	12242.5
28	14896.0
29	17400.0
30	19904.0
31	22488.0
32	25072.0
33	29442.0
34	33812.0
35	39708.0
36	45604.0
37	56912.0
38	68220.0
39	75291.0
40	82362.0
41	91947.5
42	101533.0
43	102115.5
44	102698.0
45	114154.5
46	125611.0
47	135240.5
48	144870.0
49	148437.0
50	152004.0
51	146691.0
52	141378.0
53	139860.5
54	138343.0
55	140244.5
56	142146.0
57	139105.5
58	136065.0
59	123571.0
60	111077.0
61	98575.0
62	86073.0
63	75075.0
64	64077.0
65	54850.0
66	45623.0
67	39161.0
68	32699.0
69	30255.0
70	27811.0
71	21422.5
72	15034.0
73	12958.5
74	10883.0
75	8573.0
76	6263.0
77	5545.0
78	4827.0
79	3965.0
80	3103.0
81	2611.0
82	2119.0
83	1803.5
84	1488.0
85	1087.0
86	686.0
87	491.5
88	297.0
89	236.0
90	175.0
91	150.0
92	125.0
93	102.0
94	79.0
95	80.5
96	82.0
97	69.0
98	56.0
99	56.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0059319141142091195
2	5.203433433516772E-4
3	1.5610300300550315E-4
4	0.0
5	0.0
6	2.0813733734067086E-4
7	5.2034334335167716E-5
8	0.0
9	0.0013008583583791929
10	8.845836836978511E-4
11	0.0
12	1.5610300300550315E-4
13	3.122060060110063E-4
14	2.0813733734067086E-4
15	0.0040066437438079135
16	0.0011447553553736898
17	0.0
18	1.0406866867033543E-4
19	0.0
20	7.805150150275157E-4
21	0.0
22	1.0406866867033543E-4
23	4.162746746813417E-4
24	1.0406866867033543E-4
25	0.0011967896897088575
26	0.004526987087159592
27	0.0059319141142091195
28	0.0036944377377969083
29	0.0024456137137528824
30	0.002757819719763889
31	0.006348188788890461
32	0.002393579379417715
33	0.003226128728780399
34	0.0022895107107473796
35	0.002081373373406709
36	0.006556326126231133
37	0.003226128728780399
38	0.007076669469582809
39	0.0031740943944452305
40	0.0017171330330605346
41	0.0026017167167583857
42	0.0011967896897088575
43	0.0010927210210385221
44	7.284806806923481E-4
45	9.886523523681865E-4
46	6.764463463571804E-4
47	0.001665098698725367
48	0.0015089956957198638
49	4.6830900901650947E-4
50	0.0011447553553736898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1921808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.880950156283596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55024525817535	38.1839896817433
2	13.132718172984967	13.101449212063656
3	4.443745021254802	6.6497467173733265
4	1.9917072412616244	3.9739299850912078
5	1.0305538440635895	2.5702502464551182
6	0.6150214136910881	1.8406711488823342
7	0.4304767279279479	1.5030811746449817
8	0.2905415174000603	1.159398955821073
9	0.21225856943102983	0.9528893209829933
>10	1.1183135846350283	9.877905541675094
>50	0.08550152843397071	3.008520859130661
>100	0.08236205654174887	9.050901066750917
>500	0.01068744650814628	3.77811096364994
>1k	0.005867617690746976	4.349155125735477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2704	0.1407008400422935	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2492	0.12966956116323797	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2470	0.12852480580786427	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2358	0.12269696036232547	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2310	0.12019931231423743	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2202	0.11457960420603931	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2150	0.11187381882061058	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	2124	0.11052092612789623	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2104	0.10948023944119288	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2075	0.107971243745473	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2041	0.10620207637807731	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1977	0.10287187898062658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5610300300550315E-4
2	0.0	0.0	0.0	0.0	1.5610300300550315E-4
3	0.0	0.0	0.0	0.0	1.5610300300550315E-4
4	0.0	0.0	0.0	0.0	1.5610300300550315E-4
5	0.0	0.0	0.0	0.0	1.5610300300550315E-4
6	0.0	0.0	0.0	0.0	2.601716716758386E-4
7	0.0	0.0	0.0	0.0	2.601716716758386E-4
8	0.0	0.0	0.0	0.0	2.601716716758386E-4
9	0.0	0.0	0.0	0.0	2.601716716758386E-4
10	0.0	0.0	0.0	0.0	2.601716716758386E-4
11	0.0	0.0	0.0	0.0	2.601716716758386E-4
12	0.0	0.0	0.0	0.0	7.805150150275158E-4
13	0.0	0.0	0.0	0.0	7.805150150275158E-4
14	0.0	0.0	0.0	0.0	7.805150150275158E-4
15	0.0	0.0	0.0	0.0	9.886523523681865E-4
16	0.0	0.0	0.0	0.0	0.0010406866867033544
17	0.0	0.0	0.0	0.0	0.0010406866867033544
18	0.0	0.0	0.0	0.0	0.0010927210210385221
19	0.0	0.0	0.0	5.2034334335167716E-5	0.0011447553553736898
20	0.0	0.0	0.0	1.0406866867033543E-4	0.0012488240240440252
21	0.0	0.0	0.0	1.5610300300550315E-4	0.0013008583583791929
22	0.0	0.0	0.0	2.0813733734067086E-4	0.0013008583583791929
23	0.0	0.0	0.0	3.6424034034617404E-4	0.0013008583583791929
24	0.0	0.0	0.0	6.244120120220126E-4	0.0013008583583791929
25	0.0	0.0	0.0	6.244120120220126E-4	0.0013008583583791929
26	0.0	0.0	0.0	7.284806806923481E-4	0.0014569613613846961
27	0.0	0.0	0.0	9.366180180330189E-4	0.0014569613613846961
28	0.0	0.0	0.0	0.0013008583583791929	0.0014569613613846961
29	0.0	0.0	0.0	0.0018732360360660379	0.0014569613613846961
30	0.0	0.0	0.0	0.0035903690691265725	0.0014569613613846961
31	0.0	0.0	0.0	0.007805150150275157	0.0015089956957198638
32	0.0	0.0	0.0	0.013372823924138104	0.0015610300300550315
33	0.0	0.0	0.0	0.017483536336616354	0.0015610300300550315
34	0.0	0.0	0.0	0.022322729429786952	0.0015610300300550315
35	0.0	0.0	0.0	0.029711604905380766	0.0015610300300550315
36	0.0	0.0	0.0	0.04058678078143082	0.0015610300300550315
37	0.0	0.0	0.0	0.057289802103019656	0.0015610300300550315
38	0.0	0.0	0.0	0.08226628258390016	0.0015610300300550315
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3205	0.0	23.40629	1
ATACCGT	695	0.0	20.574224	6
TATACCG	75	5.4774788E-5	20.532022	5
TACCGTC	670	0.0	20.356909	7
ACCGTCG	660	0.0	20.332035	8
CGTCGTA	665	0.0	20.179688	10
AATCACG	190	0.0	19.685514	34
CCGTCGT	685	0.0	19.269342	9
GGTATCA	1555	0.0	19.098955	1
GTCGTAG	740	0.0	17.837162	11
TTCCGCG	75	0.001291123	17.60025	29
TATAGGG	155	1.441731E-8	17.03117	2
AACGCAG	4455	0.0	16.690292	6
TCAACGC	4590	0.0	16.1994	4
GTATAGG	205	1.05501385E-10	16.09695	1
GGCGTTA	780	0.0	16.075897	42
TATACAG	260	0.0	16.075897	5
GCATTCG	835	0.0	16.07334	22
TACGACG	685	0.0	16.057367	5
TGTAGGA	555	0.0	15.854843	2
>>END_MODULE