FastQCFastQC Report
Thu 2 Feb 2017
SRR4063904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390923
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44540.13135066765007639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.014.57511
GTATCAA33650.013.0938991
CGCGCTA1001.4402758E-712.35268716
TCCAACG3250.012.27941318
CGCAATA951.0464082E-611.994822
GATATAC8350.011.7135041
GCGTTAT1354.7675712E-911.2543981
CGTCGTA2400.011.08410710
CGACCAT2750.011.05531910
GTCTTAG4850.010.9643361
GTCCTAA9650.010.9227441
GTCCTAC16100.010.7344931
CGAAATT803.7696844E-410.68856213
AGGACGT15300.010.6160395
GGACGTG15200.010.5619926
ATACCGT3250.010.522576
CCGTCGT2550.010.4317949
TAGAAAT6150.010.1929774
GTTCTAG2151.8189894E-1210.1584031
TTAGCGC750.002653579710.1312014
Produced by FastQC (version 0.11.5)