##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063904_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390923
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260574775658426	32.0	32.0	32.0	32.0	32.0
2	30.809345420111278	32.0	32.0	32.0	32.0	32.0
3	30.83127366796592	32.0	32.0	32.0	32.0	32.0
4	30.865289775084836	32.0	32.0	32.0	32.0	32.0
5	30.7483304693147	32.0	32.0	32.0	32.0	32.0
6	34.4293255848039	36.0	36.0	36.0	32.0	36.0
7	34.38235253351374	36.0	36.0	36.0	32.0	36.0
8	34.33044041401117	36.0	36.0	36.0	32.0	36.0
9	34.49766184605195	36.0	36.0	36.0	32.0	36.0
10	34.15056077652014	36.0	36.0	36.0	32.0	36.0
11	34.470124800828565	36.0	36.0	36.0	32.0	36.0
12	34.27371721504735	36.0	36.0	36.0	32.0	36.0
13	34.351081991540354	36.0	36.0	36.0	32.0	36.0
14	34.24998090490406	36.0	36.0	36.0	32.0	36.0
15	34.18211560687164	36.0	36.0	36.0	32.0	36.0
16	34.1860632046201	36.0	36.0	36.0	32.0	36.0
17	34.120385511555405	36.0	36.0	36.0	32.0	36.0
18	34.10994970985776	36.0	36.0	36.0	32.0	36.0
19	34.124076247086705	36.0	36.0	36.0	32.0	36.0
20	34.108831725167455	36.0	36.0	36.0	32.0	36.0
21	34.09966991288213	36.0	36.0	36.0	32.0	36.0
22	34.066511979186785	36.0	36.0	36.0	32.0	36.0
23	33.99795689846098	36.0	36.0	36.0	32.0	36.0
24	33.99933587403783	36.0	36.0	36.0	32.0	36.0
25	33.57653653592252	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	31.0
5	39.0
6	150.0
7	53.0
8	180.0
9	215.0
10	341.0
11	91.0
12	207.0
13	170.0
14	222.0
15	494.0
16	836.0
17	1164.0
18	1526.0
19	2167.0
20	3286.0
21	4798.0
22	7721.0
23	12289.0
24	18638.0
25	27042.0
26	38663.0
27	51023.0
28	69743.0
29	93745.0
30	122777.0
31	167506.0
32	234613.0
33	330556.0
34	700598.0
35	1500024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54311156635662	17.406886427521165	12.132909136824615	25.917092869297598
2	16.07663974712163	19.352698142961337	38.84221084542196	25.72845126449507
3	19.310116017765406	23.130002418265573	28.372595742672953	29.187285821296072
4	12.807498672137177	15.949365147383187	35.80844211093177	35.43469406954787
5	14.75038171743796	36.39158531668612	33.95635045981577	14.901682506060144
6	34.12373855426484	35.33236689470882	17.092720593548655	13.451173957477684
7	30.30284438154339	30.22370350399701	20.501675881087515	18.971776233372083
8	27.547406490242572	34.358358461125896	19.115649208640477	18.978585839991055
9	27.074581366076977	15.0497545465972	18.528914168907583	39.34674991841824
10	15.943576224443968	27.428217671321235	31.970128589411512	24.65807751482328
11	36.515023698178766	21.29413330288258	22.8778674088152	19.31297559012345
12	24.777754858555447	23.3588681012526	29.48969121905573	22.373685821136224
13	29.85380910786374	20.26821336669893	25.011656318609976	24.866321206827358
14	23.191478750437863	19.8910113908029	25.496139863796703	31.42136999496254
15	24.773427626234444	28.267362040418746	22.141815287855284	24.817395045491526
16	24.99522671411131	26.096225312078463	24.61359992516196	24.29494804864827
17	23.731255328271704	26.41503699137414	25.587862001562222	24.265845678791937
18	23.923856329422698	25.8822546785551	26.780616354202238	23.41327263781997
19	25.528914046579438	25.08717228312436	25.21764665085785	24.16626701943835
20	25.552508730967556	25.249016668599833	25.44956403452307	23.748910565909544
21	25.96391082002512	24.741086016224845	24.703870635677657	24.59113252807238
22	25.35690632780487	25.21199521645765	25.24021008864664	24.190888367090835
23	24.59482824011232	25.376291530640344	25.312834716249167	24.716045512998168
24	24.7916878793379	25.158744564022424	25.53748850826593	24.512079048373746
25	24.838177291342266	25.180239154071277	25.60372885797969	24.37785469660677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	316.5
3	541.0
4	541.0
5	541.0
6	1320.0
7	2099.0
8	2099.0
9	2099.0
10	2854.0
11	3609.0
12	3609.0
13	3609.0
14	5824.0
15	8039.0
16	8039.0
17	8039.0
18	14589.5
19	21140.0
20	21140.0
21	21140.0
22	34081.0
23	47022.0
24	47022.0
25	47022.0
26	73294.0
27	99566.0
28	99566.0
29	99566.0
30	134557.5
31	169549.0
32	169549.0
33	169549.0
34	200573.0
35	231597.0
36	231597.0
37	231597.0
38	265565.0
39	299533.0
40	299533.0
41	299533.0
42	337570.0
43	375607.0
44	375607.0
45	375607.0
46	408198.0
47	440789.0
48	440789.0
49	440789.0
50	447888.5
51	454988.0
52	454988.0
53	454988.0
54	427123.5
55	399259.0
56	399259.0
57	399259.0
58	362973.5
59	326688.0
60	326688.0
61	326688.0
62	278675.5
63	230663.0
64	230663.0
65	230663.0
66	185922.0
67	141181.0
68	141181.0
69	141181.0
70	106911.0
71	72641.0
72	72641.0
73	72641.0
74	53954.5
75	35268.0
76	35268.0
77	35268.0
78	26591.0
79	17914.0
80	17914.0
81	17914.0
82	12371.0
83	6828.0
84	6828.0
85	6828.0
86	4912.5
87	2997.0
88	2997.0
89	2997.0
90	2118.0
91	1239.0
92	1239.0
93	1239.0
94	896.5
95	554.0
96	554.0
97	554.0
98	1037.0
99	1520.0
100	1520.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03830815385663431
2	0.005957080122432742
3	0.001857901226303281
4	0.004010707409162638
5	0.009613901583728088
6	0.013801551966824373
7	0.022589719672195446
8	0.036863119569509536
9	0.04969148517969886
10	0.05889251982424844
11	0.05438047398894048
12	0.06517399539889286
13	0.06496756192930361
14	0.06818202595576485
15	0.060455516093995645
16	0.06700240612954055
17	0.06369947061611249
18	0.07797287051342658
19	0.07425706806082003
20	0.07950637628751818
21	0.07487636846958777
22	0.07782541803514854
23	0.08233746387045651
24	0.07720611762638079
25	0.07806134200039341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3390923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.7304290983168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.55361230364896	24.455514293754057
2	16.54026949791146	13.143040091076461
3	7.753480866458146	9.241473654899137
4	4.272977194783478	6.790688699042782
5	2.60089112188792	5.166726015530481
6	1.6975323061947558	4.046621216002376
7	1.1847662395834802	3.2949889755895607
8	0.8382535634917421	2.664333901657604
9	0.6242757522066915	2.232246915976171
>10	2.7499891176778823	18.299234925934055
>50	0.11713545174517283	3.169579055784588
>100	0.05859980561020441	4.459984929690838
>500	0.006125235108135702	1.6548952454008774
>1k	0.002091543691912745	1.3806720796610432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4454	0.13135066765007639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9490495655607633E-5
2	0.0	0.0	0.0	0.0	5.8980991311215265E-5
3	0.0	0.0	0.0	0.0	5.8980991311215265E-5
4	0.0	0.0	0.0	0.0	5.8980991311215265E-5
5	0.0	5.8980991311215265E-5	0.0	0.0	5.8980991311215265E-5
6	0.0	5.8980991311215265E-5	0.0	0.0	5.8980991311215265E-5
7	0.0	1.4745247827803817E-4	0.0	0.0	5.8980991311215265E-5
8	0.0	1.4745247827803817E-4	0.0	2.9490495655607633E-5	5.8980991311215265E-5
9	0.0	1.4745247827803817E-4	0.0	2.9490495655607633E-5	5.8980991311215265E-5
10	0.0	1.4745247827803817E-4	0.0	2.9490495655607633E-5	5.8980991311215265E-5
11	0.0	1.4745247827803817E-4	0.0	5.8980991311215265E-5	5.8980991311215265E-5
12	0.0	1.7694297393364578E-4	0.0	8.847148696682289E-5	2.654144609004687E-4
13	0.0	2.3592396524486106E-4	0.0	8.847148696682289E-5	2.654144609004687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1075	0.0	14.5751	1
GTATCAA	3365	0.0	13.093899	1
CGCGCTA	100	1.4402758E-7	12.352687	16
TCCAACG	325	0.0	12.279413	18
CGCAATA	95	1.0464082E-6	11.99482	2
GATATAC	835	0.0	11.713504	1
GCGTTAT	135	4.7675712E-9	11.254398	1
CGTCGTA	240	0.0	11.084107	10
CGACCAT	275	0.0	11.055319	10
GTCTTAG	485	0.0	10.964336	1
GTCCTAA	965	0.0	10.922744	1
GTCCTAC	1610	0.0	10.734493	1
CGAAATT	80	3.7696844E-4	10.688562	13
AGGACGT	1530	0.0	10.616039	5
GGACGTG	1520	0.0	10.561992	6
ATACCGT	325	0.0	10.52257	6
CCGTCGT	255	0.0	10.431794	9
TAGAAAT	615	0.0	10.192977	4
GTTCTAG	215	1.8189894E-12	10.158403	1
TTAGCGC	75	0.0026535797	10.131201	4
>>END_MODULE