FastQCFastQC Report
Thu 2 Feb 2017
SRR4063904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390923
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35900.029.1691361
GGTATCA14600.023.0541761
TAGGACG18600.019.7512864
TATCAAC55550.018.8105452
GTAGGAC21600.018.4341493
TCAACGC56350.018.231184
ATCAACG57200.018.0374453
TTAGGAC14800.017.9854743
CAACGCA57700.017.8808785
AACGCAG58500.017.6739586
GTCCTAC18250.017.4789831
TGTAGGA25450.016.9418222
GGACGTG21450.016.8193196
AGGACGT22300.016.276875
CGCGTAA2301.8189894E-1216.26067410
GTATAGA5300.016.1882441
GACGTGA12700.015.9358777
GATATAC11150.014.9951141
TAGCGTA2950.014.9141947
CTGTAGG28200.014.900311
Produced by FastQC (version 0.11.5)