##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390923
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35597299024484	32.0	32.0	32.0	32.0	32.0
2	31.416940461343415	32.0	32.0	32.0	32.0	32.0
3	31.494051914478742	32.0	32.0	32.0	32.0	32.0
4	31.57471254876622	32.0	32.0	32.0	32.0	32.0
5	31.52646314882408	32.0	32.0	32.0	32.0	32.0
6	35.12264802238211	36.0	36.0	36.0	36.0	36.0
7	35.144040133025726	36.0	36.0	36.0	36.0	36.0
8	35.08767819263369	36.0	36.0	36.0	36.0	36.0
9	35.1994589673667	36.0	36.0	36.0	36.0	36.0
10	35.05829268314261	36.0	36.0	36.0	36.0	36.0
11	35.19389175159684	36.0	36.0	36.0	36.0	36.0
12	35.118047505059835	36.0	36.0	36.0	36.0	36.0
13	35.150130510188525	36.0	36.0	36.0	36.0	36.0
14	35.09467835158746	36.0	36.0	36.0	36.0	36.0
15	35.070289711680275	36.0	36.0	36.0	36.0	36.0
16	35.08785336617788	36.0	36.0	36.0	36.0	36.0
17	35.06314151043831	36.0	36.0	36.0	36.0	36.0
18	35.05679338634348	36.0	36.0	36.0	36.0	36.0
19	35.04636879103418	36.0	36.0	36.0	36.0	36.0
20	35.044827322826265	36.0	36.0	36.0	36.0	36.0
21	35.036926819040126	36.0	36.0	36.0	36.0	36.0
22	35.018971235855254	36.0	36.0	36.0	36.0	36.0
23	34.97416603090073	36.0	36.0	36.0	36.0	36.0
24	34.95238081195002	36.0	36.0	36.0	36.0	36.0
25	34.92394607603888	36.0	36.0	36.0	36.0	36.0
26	34.87032793136264	36.0	36.0	36.0	32.0	36.0
27	34.85731878901408	36.0	36.0	36.0	32.0	36.0
28	34.83838205703875	36.0	36.0	36.0	32.0	36.0
29	34.81274832840498	36.0	36.0	36.0	32.0	36.0
30	34.79288117129171	36.0	36.0	36.0	32.0	36.0
31	34.78425520131245	36.0	36.0	36.0	32.0	36.0
32	34.74959207271884	36.0	36.0	36.0	32.0	36.0
33	34.7444734073879	36.0	36.0	36.0	32.0	36.0
34	34.73076504538735	36.0	36.0	36.0	32.0	36.0
35	34.704787457574234	36.0	36.0	36.0	32.0	36.0
36	34.67686172761812	36.0	36.0	36.0	32.0	36.0
37	34.65776515715633	36.0	36.0	36.0	32.0	36.0
38	34.62989722857169	36.0	36.0	36.0	32.0	36.0
39	34.60973428178699	36.0	36.0	36.0	32.0	36.0
40	34.611821324164545	36.0	36.0	36.0	32.0	36.0
41	34.60095525613528	36.0	36.0	36.0	32.0	36.0
42	34.55341097394426	36.0	36.0	36.0	32.0	36.0
43	34.54719762141458	36.0	36.0	36.0	32.0	36.0
44	34.495537940554826	36.0	36.0	36.0	32.0	36.0
45	34.471864150262334	36.0	36.0	36.0	32.0	36.0
46	34.4441427894411	36.0	36.0	36.0	32.0	36.0
47	34.425968386778464	36.0	36.0	36.0	32.0	36.0
48	34.386531336748135	36.0	36.0	36.0	32.0	36.0
49	34.36818087582643	36.0	36.0	36.0	32.0	36.0
50	33.79943130528178	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	4.0
19	13.0
20	24.0
21	110.0
22	340.0
23	967.0
24	2410.0
25	5509.0
26	11460.0
27	21166.0
28	35145.0
29	54547.0
30	79304.0
31	113881.0
32	165575.0
33	269446.0
34	572872.0
35	2058147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63608690946081	18.259817041717284	12.716830516287784	26.387265532534126
2	15.400029903486057	20.016520643874546	38.19642461652613	26.387024836113266
3	18.56549168009475	23.79380247863107	28.609303005924087	29.0314028353501
4	12.512080044282929	16.622671762231107	35.75392304691082	35.11132514657513
5	14.384785499405325	36.8776288933721	33.62801219608939	15.109573411133193
6	34.243746671251465	35.61317096216536	16.855779887074696	13.287302479508476
7	30.27974979083866	30.355392912195295	20.453988486320686	18.91086881064536
8	27.601637548725687	34.498522820636985	18.927300598480297	18.972539032157034
9	27.052441005674932	14.907470840139586	18.441217419564317	39.59887073462116
10	15.837032947653332	27.677597707271296	31.926034845088054	24.55933449998732
11	36.671029295277215	21.225849488723124	22.838095361674494	19.265025854325167
12	24.76479090181747	23.368561737983125	29.483541187506983	22.38310617269242
13	29.887790951259884	20.276929183760195	24.97249257793573	24.86278728704419
14	23.187853168575153	19.858509915777745	25.43114545391811	31.52249146172899
15	24.85290166596918	28.356689460973193	22.06851032467952	24.721898548378107
16	25.06471752253833	26.16720094134578	24.489293371356784	24.278788164759106
17	23.720296804144475	26.534014485141654	25.46701886182612	24.278669848887752
18	24.052113413736482	25.899993865957004	26.66978677122851	23.378105949078005
19	25.590849453084015	25.079454767920122	25.25067658569658	24.079019193299285
20	25.705528655102178	25.221349984974477	25.344768655609418	23.72835270431393
21	26.070364912444195	24.71893345853032	24.698142659093115	24.512558969932375
22	25.447342252064043	25.174672566350804	25.208586706364887	24.169398475220266
23	24.625814349418455	25.345977775202254	25.243320572698018	24.784887302681266
24	24.843788337768718	25.253589594775683	25.43404252307344	24.46857954438216
25	24.904449469694555	25.097762348136587	25.630898918155665	24.366889264013192
26	24.243337601813877	25.805442224218506	25.78055094660585	24.170669227361767
27	24.639881982862505	25.496520771263935	25.468178490893017	24.395418754980543
28	24.32096147590515	25.66923254100007	25.342818643577438	24.666987339517345
29	24.574302526772808	25.218713790389245	25.117913017013155	25.089070665824792
30	24.46988723565142	25.489384768378326	25.454525614694873	24.586202381275385
31	25.16618343040823	24.963480787487825	25.15075880037078	24.71957698173317
32	24.66701800961956	25.569430685805912	25.093554645969874	24.669996658604653
33	24.34170867313053	25.519442907582636	25.491012602529	24.647835816757837
34	24.738308292207613	25.398354735223492	25.60019369934488	24.263143273224014
35	24.741876139475654	25.452649422822955	25.623434372985365	24.182040064716023
36	24.383377090973223	25.646587700396918	25.498149910076133	24.471885298553726
37	24.84052464222371	25.262725145584852	25.073389630665982	24.823360581525456
38	24.70939269015041	25.584680023759397	25.1160394236163	24.58988786247389
39	25.069585882161416	24.975831476825057	25.23589009470994	24.718692546303586
40	25.541187077039773	25.068825270958495	25.330855279513916	24.059132372487824
41	24.844904772296967	24.94240331247309	25.946791631522757	24.265900283707186
42	25.025222252204827	25.61501450224206	25.401292594345676	23.95847065120744
43	24.449058133962474	25.21596932945036	25.757157075975968	24.5778154606112
44	24.20040502843958	25.477008428213026	25.412924636222	24.90966190712539
45	24.425059733862124	25.492896521663738	25.45544305096562	24.626600693508518
46	24.54521513384838	24.99214807680228	25.300267864857855	25.162368924491485
47	24.88469695034898	25.133749768125778	25.437567663772505	24.543985617752735
48	24.593007172841233	25.374228142475356	25.06616342942145	24.96660125526196
49	24.64253727535917	25.278088821506184	25.391509602281154	24.687864300853487
50	24.51215423030679	25.74198356354418	25.049065477751036	24.69679672839799
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	20.0
2	35.0
3	103.0
4	171.0
5	207.0
6	243.0
7	243.0
8	243.0
9	339.5
10	436.0
11	683.5
12	931.0
13	1703.5
14	2476.0
15	3310.0
16	4144.0
17	4231.0
18	4318.0
19	4562.0
20	4806.0
21	5820.0
22	6834.0
23	8998.0
24	11162.0
25	15382.5
26	19603.0
27	26982.0
28	34361.0
29	41968.0
30	49575.0
31	58274.5
32	66974.0
33	80155.0
34	93336.0
35	110181.5
36	127027.0
37	137609.5
38	148192.0
39	155460.0
40	162728.0
41	171210.5
42	179693.0
43	185643.0
44	191593.0
45	206126.5
46	220660.0
47	232213.0
48	243766.0
49	251459.5
50	259153.0
51	258868.0
52	258583.0
53	255653.0
54	252723.0
55	244179.0
56	235635.0
57	223331.5
58	211028.0
59	192769.5
60	174511.0
61	153717.0
62	132923.0
63	115375.5
64	97828.0
65	81568.0
66	65308.0
67	55687.5
68	46067.0
69	38502.5
70	30938.0
71	24711.5
72	18485.0
73	15747.0
74	13009.0
75	10509.5
76	8010.0
77	6766.0
78	5522.0
79	4499.0
80	3476.0
81	2778.0
82	2080.0
83	1601.0
84	1122.0
85	834.5
86	547.0
87	418.5
88	290.0
89	220.0
90	150.0
91	111.0
92	72.0
93	58.0
94	44.0
95	44.5
96	45.0
97	42.5
98	40.0
99	31.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009260015635860796
2	4.1286693917850684E-4
3	5.60319417456545E-4
4	0.0
5	0.0
6	2.6541446090046873E-4
7	0.0
8	2.9490495655607633E-5
9	0.0013565628001579511
10	8.552243740126214E-4
11	2.9490495655607633E-5
12	1.1796198262243053E-4
13	5.898099131121526E-4
14	4.1286693917850684E-4
15	0.004836441287519652
16	9.731863566350519E-4
17	0.0
18	3.2439545221168395E-4
19	0.0
20	7.667528870457985E-4
21	0.0
22	2.0643346958925342E-4
23	7.667528870457985E-4
24	3.2439545221168395E-4
25	0.0013860532958135587
26	0.005219817731042551
27	0.0067533235051341475
28	0.004010707409162638
29	0.00224127766982618
30	0.0039812169135070305
31	0.007136699948657048
32	0.0036568214612953467
33	0.0051608367397313355
34	0.002978540061216371
35	0.003214464026461232
36	0.008168867296603315
37	0.003450387991706093
38	0.007814981348736023
39	0.004394083852685537
40	0.0026541446090046867
41	0.003037521052527586
42	0.002005353704581319
43	0.0014155437914691663
44	0.0014745247827803816
45	0.001445034287124774
46	0.0010026768522906596
47	0.00224127766982618
48	0.0022707681654817874
49	2.949049565560763E-4
50	0.0013565628001579511
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3390923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.50849674955882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.99072596254044	26.776410698310006
2	16.113523092055893	13.699232879651976
3	7.4577377426038245	9.510516617716105
4	4.1305704941159345	7.023373696918029
5	2.506002415210482	5.3263197760680665
6	1.6377087890216449	4.17699232409103
7	1.1033074343956155	3.2829958342139074
8	0.7889590963108715	2.6829972144852436
9	0.5907710754288629	2.2601511305641067
>10	2.5402567331996773	17.84986243423754
>50	0.09399223726589509	2.6997682478561433
>100	0.04233085263554771	3.349958263095196
>500	0.0034167743327760043	1.007475166722015
>1k	6.973008825581382E-4	0.35394571607056385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1796198262243053E-4
2	0.0	0.0	0.0	0.0	1.1796198262243053E-4
3	0.0	0.0	0.0	0.0	1.1796198262243053E-4
4	0.0	0.0	0.0	0.0	1.1796198262243053E-4
5	0.0	2.0643346958925342E-4	0.0	0.0	1.1796198262243053E-4
6	0.0	2.0643346958925342E-4	0.0	0.0	1.1796198262243053E-4
7	0.0	2.0643346958925342E-4	0.0	0.0	1.1796198262243053E-4
8	0.0	2.0643346958925342E-4	0.0	2.9490495655607633E-5	1.4745247827803817E-4
9	0.0	2.0643346958925342E-4	0.0	2.9490495655607633E-5	1.7694297393364578E-4
10	0.0	2.0643346958925342E-4	0.0	5.8980991311215265E-5	1.7694297393364578E-4
11	0.0	2.0643346958925342E-4	0.0	8.847148696682289E-5	1.7694297393364578E-4
12	0.0	2.654144609004687E-4	0.0	1.1796198262243053E-4	3.5388594786729156E-4
13	0.0	2.9490495655607634E-4	0.0	1.1796198262243053E-4	4.718479304897221E-4
14	0.0	2.9490495655607634E-4	0.0	1.4745247827803817E-4	5.308289218009373E-4
15	0.0	2.9490495655607634E-4	0.0	1.4745247827803817E-4	7.077718957345831E-4
16	0.0	3.2439545221168395E-4	0.0	2.0643346958925342E-4	7.667528870457985E-4
17	0.0	3.2439545221168395E-4	0.0	2.0643346958925342E-4	7.667528870457985E-4
18	0.0	4.1286693917850684E-4	0.0	2.654144609004687E-4	8.257338783570137E-4
19	0.0	4.1286693917850684E-4	0.0	2.9490495655607634E-4	8.257338783570137E-4
20	0.0	4.423574348341145E-4	0.0	4.423574348341145E-4	9.142053653238366E-4
21	0.0	4.423574348341145E-4	0.0	4.423574348341145E-4	9.731863566350519E-4
22	0.0	4.423574348341145E-4	0.0	6.487909044233679E-4	9.731863566350519E-4
23	0.0	4.718479304897221E-4	0.0	7.372623913901908E-4	0.0010911483392574825
24	0.0	4.718479304897221E-4	0.0	9.731863566350519E-4	0.00112063883491309
25	0.0	5.013384261453298E-4	0.0	0.0014155437914691663	0.00112063883491309
26	0.0	5.013384261453298E-4	0.0	0.0017399392436808503	0.0011501293305686976
27	0.0	5.013384261453298E-4	0.0	0.0021233156872037494	0.0011796198262243054
28	0.0	5.308289218009373E-4	0.0	0.0030965020438388016	0.0011796198262243054
29	0.0	5.308289218009373E-4	0.0	0.00560319417456545	0.001209110321879913
30	0.0	6.193004087677602E-4	0.0	0.009525430096761265	0.001209110321879913
31	0.0	6.193004087677602E-4	0.0	0.021616533315560394	0.0012386008175355205
32	0.0	6.193004087677602E-4	0.0	0.0359489142041857	0.0012680913131911282
33	0.0	6.193004087677602E-4	0.0	0.05290594920616009	0.0012680913131911282
34	0.0	6.193004087677602E-4	0.0	0.07095413254739197	0.001445034287124774
35	0.0	6.487909044233679E-4	0.0	0.09100766959320515	0.001445034287124774
36	0.0	6.487909044233679E-4	0.0	0.12052765574446839	0.0014745247827803816
37	0.0	6.487909044233679E-4	0.0	0.16747652482819575	0.0014745247827803816
38	0.0	6.487909044233679E-4	0.0	0.23542262681871573	0.001533505774091597
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3590	0.0	29.169136	1
GGTATCA	1460	0.0	23.054176	1
TAGGACG	1860	0.0	19.751286	4
TATCAAC	5555	0.0	18.810545	2
GTAGGAC	2160	0.0	18.434149	3
TCAACGC	5635	0.0	18.23118	4
ATCAACG	5720	0.0	18.037445	3
TTAGGAC	1480	0.0	17.985474	3
CAACGCA	5770	0.0	17.880878	5
AACGCAG	5850	0.0	17.673958	6
GTCCTAC	1825	0.0	17.478983	1
TGTAGGA	2545	0.0	16.941822	2
GGACGTG	2145	0.0	16.819319	6
AGGACGT	2230	0.0	16.27687	5
CGCGTAA	230	1.8189894E-12	16.260674	10
GTATAGA	530	0.0	16.188244	1
GACGTGA	1270	0.0	15.935877	7
GATATAC	1115	0.0	14.995114	1
TAGCGTA	295	0.0	14.914194	7
CTGTAGG	2820	0.0	14.90031	1
>>END_MODULE