FastQCFastQC Report
Thu 2 Feb 2017
SRR4063903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413310
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39670.2806885962739951No Hit
TATCAACGCAGAGTACTTTTTTTTT28460.20137124905364007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20440.14462502918680262No Hit
GTACATGGGGTGGTATCAACGCAAA15910.11257261322710516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG250.00604643518.9920371
GCTCGTA707.253044E-613.5729479
CTAGACA1351.8189894E-1213.3666594
AATCGCT655.4406573E-513.15671415
CGCCAGT1950.013.15671418
TCTAGAC1102.757588E-912.9504913
ATACCGT1850.012.3230856
ACGTCTG550.003066452512.0918337
ACCGTCG1650.012.0918328
CGAACGT550.003074593812.0875534
ACTAGAC550.00307540912.0871253
CGAGCCG2050.012.05140815
GTAAGAC1201.0055373E-811.87128453
GTCCTAA3850.011.8391911
CGCATCG2200.011.65998213
TTAGGGT907.4821855E-611.6078884
ACCGACC752.0720888E-411.400878
ACCGTGG752.0720888E-411.400878
TACACCC752.0789134E-411.3968355
GTCCTAT3550.011.2347261
Produced by FastQC (version 0.11.5)