##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063903_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413310
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199083711287685	32.0	32.0	32.0	32.0	32.0
2	30.728138200394817	32.0	32.0	32.0	32.0	32.0
3	30.767895932244166	32.0	32.0	32.0	32.0	32.0
4	30.79247581917626	32.0	32.0	32.0	32.0	32.0
5	30.68132398412238	32.0	32.0	32.0	32.0	32.0
6	34.345870332764925	36.0	36.0	36.0	32.0	36.0
7	34.270292434073205	36.0	36.0	36.0	32.0	36.0
8	34.2188705945617	36.0	36.0	36.0	32.0	36.0
9	34.38060368921185	36.0	36.0	36.0	32.0	36.0
10	34.03359984716729	36.0	36.0	36.0	32.0	36.0
11	34.3793138094261	36.0	36.0	36.0	32.0	36.0
12	34.1575719410462	36.0	36.0	36.0	32.0	36.0
13	34.255175439217155	36.0	36.0	36.0	32.0	36.0
14	34.1448599387254	36.0	36.0	36.0	32.0	36.0
15	34.079404376959054	36.0	36.0	36.0	32.0	36.0
16	34.06978086902378	36.0	36.0	36.0	32.0	36.0
17	33.99571007068513	36.0	36.0	36.0	32.0	36.0
18	33.992970402813256	36.0	36.0	36.0	32.0	36.0
19	33.99513553289795	36.0	36.0	36.0	32.0	36.0
20	33.974614203536376	36.0	36.0	36.0	32.0	36.0
21	33.952403931196976	36.0	36.0	36.0	32.0	36.0
22	33.933006912849976	36.0	36.0	36.0	32.0	36.0
23	33.88942694808641	36.0	36.0	36.0	32.0	36.0
24	33.86544848617784	36.0	36.0	36.0	32.0	36.0
25	33.46286377369438	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	15.0
5	15.0
6	69.0
7	27.0
8	91.0
9	89.0
10	130.0
11	38.0
12	92.0
13	76.0
14	119.0
15	263.0
16	410.0
17	567.0
18	775.0
19	1112.0
20	1615.0
21	2518.0
22	3844.0
23	6091.0
24	8873.0
25	12685.0
26	17888.0
27	23364.0
28	31377.0
29	41420.0
30	53821.0
31	71804.0
32	99542.0
33	138513.0
34	289147.0
35	606914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30677654057168	17.47622226107416	11.701684137061138	25.51531706129302
2	15.996787523704395	19.9523789306841	39.08522260904022	24.965610936571284
3	18.656521043865148	25.04156978842216	28.712022902653818	27.589886265058876
4	12.280710444031058	16.06883956704265	36.704152181804155	34.94629780712213
5	14.107665363454633	37.337909317671205	33.885399897394166	14.66902542148
6	32.87894581529689	36.32750718096455	17.65530096361857	13.138246040119991
7	29.428600546940025	30.757591199134858	21.432787525690895	18.38102072823422
8	27.690185327928436	33.82413417960027	19.568613377897538	18.917067114573754
9	27.801363312329347	14.15153310694052	18.717404708659068	39.32969887207107
10	15.807654049155548	27.110598213748403	32.07833169906298	25.003416038033073
11	36.39260468030428	21.19142097606043	23.63720031291703	18.778774030718257
12	25.05970439861935	23.55479245950969	29.793291441720505	21.592211700150457
13	29.730807083129772	20.145924934684253	25.47165402833535	24.65161395385062
14	23.399191966681723	20.231137746598147	25.872006162917984	30.49766412380215
15	24.670320878710083	28.273391889461735	22.675749804946637	24.380537426881542
16	24.722674783744036	25.937970631530426	25.253924186327424	24.08543039839811
17	23.211366370310476	26.289227309705552	26.002901491598912	24.496504828385053
18	23.80332266189674	25.56825222829085	27.512903799158888	23.115521310653524
19	25.304110582318923	24.85708803009135	25.838724912956994	24.000076474632728
20	25.64356765170125	24.80620209932678	25.625014605213124	23.925215643758847
21	26.432673835907146	24.416652445729717	24.814744267428203	24.335929450934934
22	25.574134540554283	25.130136690438608	25.2174491863226	24.07827958268451
23	24.055284331762643	25.03693034151537	26.01524801751695	24.892537309205036
24	24.609633628121816	25.193474857297023	26.072741872781386	24.124149641799775
25	24.42705441814618	25.1590453314601	26.0332281528648	24.38067209752892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	155.0
1	155.0
2	433.0
3	711.0
4	711.0
5	711.0
6	1765.0
7	2819.0
8	2819.0
9	2819.0
10	2816.0
11	2813.0
12	2813.0
13	2813.0
14	3158.0
15	3503.0
16	3503.0
17	3503.0
18	6288.5
19	9074.0
20	9074.0
21	9074.0
22	15535.5
23	21997.0
24	21997.0
25	21997.0
26	34492.0
27	46987.0
28	46987.0
29	46987.0
30	60853.0
31	74719.0
32	74719.0
33	74719.0
34	88074.0
35	101429.0
36	101429.0
37	101429.0
38	113760.0
39	126091.0
40	126091.0
41	126091.0
42	140866.5
43	155642.0
44	155642.0
45	155642.0
46	170398.0
47	185154.0
48	185154.0
49	185154.0
50	187479.0
51	189804.0
52	189804.0
53	189804.0
54	172941.5
55	156079.0
56	156079.0
57	156079.0
58	140855.0
59	125631.0
60	125631.0
61	125631.0
62	109224.5
63	92818.0
64	92818.0
65	92818.0
66	75756.5
67	58695.0
68	58695.0
69	58695.0
70	43935.5
71	29176.0
72	29176.0
73	29176.0
74	21971.5
75	14767.0
76	14767.0
77	14767.0
78	11638.5
79	8510.0
80	8510.0
81	8510.0
82	5814.5
83	3119.0
84	3119.0
85	3119.0
86	2431.0
87	1743.0
88	1743.0
89	1743.0
90	1238.5
91	734.0
92	734.0
93	734.0
94	532.0
95	330.0
96	330.0
97	330.0
98	570.0
99	810.0
100	810.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03813742208008151
2	0.004952911958452144
3	0.0016981412428978783
4	0.003750061911399481
5	0.009552044491300564
6	0.014080421139028238
7	0.02533060687322668
8	0.036580792607425125
9	0.05101499317205708
10	0.060496281778236904
11	0.056250928670992205
12	0.0661567525878965
13	0.06651053201350023
14	0.07018983803977896
15	0.06198215536577255
16	0.06927001153320927
17	0.066015240817655
18	0.07988339430132102
19	0.07620408827504227
20	0.08087397669301144
21	0.0759210647345593
22	0.08073246492276995
23	0.08434101506392794
24	0.0786097883691476
25	0.08023717372692474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1413310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.85031065677541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92664903144444	35.855657987183605
2	15.450348664815758	15.404093613931213
3	5.578651654863575	8.342925541226505
4	2.5109046423889607	5.0067750581051715
5	1.3523910292874122	3.370855646970688
6	0.7635469342751128	2.283783112478571
7	0.5109064056471688	1.782819012663352
8	0.32626550868486826	1.3011549571625232
9	0.237787565856323	1.0668405625430528
>10	1.166675461790957	10.238646682420237
>50	0.0883833107975965	3.105662944355696
>100	0.07780040162891749	7.965285131106898
>500	0.007409532396860995	2.5513349174328153
>1k	0.002279856122111076	1.724164832419587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3967	0.2806885962739951	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2846	0.20137124905364007	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2044	0.14462502918680262	No Hit
GTACATGGGGTGGTATCAACGCAAA	1591	0.11257261322710516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.075588512074492E-5
2	0.0	0.0	0.0	0.0	7.075588512074492E-5
3	0.0	0.0	0.0	0.0	7.075588512074492E-5
4	0.0	0.0	0.0	0.0	7.075588512074492E-5
5	0.0	0.0	0.0	0.0	7.075588512074492E-5
6	0.0	0.0	0.0	0.0	1.4151177024148985E-4
7	0.0	0.0	0.0	0.0	1.4151177024148985E-4
8	0.0	0.0	0.0	0.0	1.4151177024148985E-4
9	0.0	0.0	0.0	0.0	1.4151177024148985E-4
10	0.0	0.0	0.0	0.0	1.4151177024148985E-4
11	0.0	0.0	0.0	0.0	1.4151177024148985E-4
12	0.0	0.0	0.0	0.0	3.537794256037246E-4
13	0.0	0.0	0.0	0.0	4.952911958452144E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	25	0.006046435	18.992037	1
GCTCGTA	70	7.253044E-6	13.572947	9
CTAGACA	135	1.8189894E-12	13.366659	4
AATCGCT	65	5.4406573E-5	13.156714	15
CGCCAGT	195	0.0	13.156714	18
TCTAGAC	110	2.757588E-9	12.950491	3
ATACCGT	185	0.0	12.323085	6
ACGTCTG	55	0.0030664525	12.091833	7
ACCGTCG	165	0.0	12.091832	8
CGAACGT	55	0.0030745938	12.087553	4
ACTAGAC	55	0.003075409	12.087125	3
CGAGCCG	205	0.0	12.051408	15
GTAAGAC	120	1.0055373E-8	11.8712845	3
GTCCTAA	385	0.0	11.839191	1
CGCATCG	220	0.0	11.659982	13
TTAGGGT	90	7.4821855E-6	11.607888	4
ACCGACC	75	2.0720888E-4	11.40087	8
ACCGTGG	75	2.0720888E-4	11.40087	8
TACACCC	75	2.0789134E-4	11.396835	5
GTCCTAT	355	0.0	11.234726	1
>>END_MODULE