##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413310
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294774677883833	32.0	32.0	32.0	32.0	32.0
2	31.403581662904813	32.0	32.0	32.0	32.0	32.0
3	31.486042694101084	32.0	32.0	32.0	32.0	32.0
4	31.57693570412719	32.0	32.0	32.0	32.0	32.0
5	31.526445012063878	32.0	32.0	32.0	32.0	32.0
6	35.110316208050605	36.0	36.0	36.0	36.0	36.0
7	35.13042786083732	36.0	36.0	36.0	36.0	36.0
8	35.08225371645287	36.0	36.0	36.0	36.0	36.0
9	35.180299438905834	36.0	36.0	36.0	36.0	36.0
10	35.05032866108638	36.0	36.0	36.0	36.0	36.0
11	35.18628114143394	36.0	36.0	36.0	36.0	36.0
12	35.102486361803145	36.0	36.0	36.0	36.0	36.0
13	35.134710007004834	36.0	36.0	36.0	36.0	36.0
14	35.090094883641946	36.0	36.0	36.0	36.0	36.0
15	35.057845058762766	36.0	36.0	36.0	36.0	36.0
16	35.06690464229362	36.0	36.0	36.0	36.0	36.0
17	35.04064996356072	36.0	36.0	36.0	36.0	36.0
18	35.03500788928119	36.0	36.0	36.0	36.0	36.0
19	35.029202368907036	36.0	36.0	36.0	36.0	36.0
20	35.019963065427966	36.0	36.0	36.0	36.0	36.0
21	35.00981101103084	36.0	36.0	36.0	36.0	36.0
22	34.99053569280625	36.0	36.0	36.0	36.0	36.0
23	34.94926378501532	36.0	36.0	36.0	36.0	36.0
24	34.922149422278196	36.0	36.0	36.0	36.0	36.0
25	34.896534376746786	36.0	36.0	36.0	32.0	36.0
26	34.83707608380327	36.0	36.0	36.0	32.0	36.0
27	34.822738818801255	36.0	36.0	36.0	32.0	36.0
28	34.79770538664553	36.0	36.0	36.0	32.0	36.0
29	34.76040146889218	36.0	36.0	36.0	32.0	36.0
30	34.74315330677629	36.0	36.0	36.0	32.0	36.0
31	34.72892359071966	36.0	36.0	36.0	32.0	36.0
32	34.691008342118856	36.0	36.0	36.0	32.0	36.0
33	34.66704473894616	36.0	36.0	36.0	32.0	36.0
34	34.656335128174284	36.0	36.0	36.0	32.0	36.0
35	34.6116909948985	36.0	36.0	36.0	32.0	36.0
36	34.585451882460326	36.0	36.0	36.0	32.0	36.0
37	34.55942503767751	36.0	36.0	36.0	32.0	36.0
38	34.51832860448168	36.0	36.0	36.0	32.0	36.0
39	34.49270931359716	36.0	36.0	36.0	32.0	36.0
40	34.47790435219449	36.0	36.0	36.0	32.0	36.0
41	34.458940359864435	36.0	36.0	36.0	32.0	36.0
42	34.39946650062619	36.0	36.0	36.0	32.0	36.0
43	34.391183816713955	36.0	36.0	36.0	32.0	36.0
44	34.309518789225294	36.0	36.0	36.0	32.0	36.0
45	34.27197147122712	36.0	36.0	36.0	32.0	36.0
46	34.23408735521577	36.0	36.0	36.0	32.0	36.0
47	34.197156320976994	36.0	36.0	36.0	32.0	36.0
48	34.135765684810835	36.0	36.0	36.0	32.0	36.0
49	34.11426297132264	36.0	36.0	36.0	32.0	36.0
50	33.53562488059944	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	6.0
20	13.0
21	51.0
22	156.0
23	454.0
24	1143.0
25	2708.0
26	5557.0
27	9901.0
28	16552.0
29	25056.0
30	35798.0
31	50704.0
32	72948.0
33	117237.0
34	242037.0
35	832987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50584607241445	18.319085234554063	12.226687357722456	25.948381335309033
2	15.30517807184007	20.865403529030516	38.480201739750626	25.349216659378786
3	17.899937805180922	25.673936408450853	28.981865858723655	27.44425992764457
4	11.942036778909086	16.79270648336175	36.763979594002734	34.501277143726426
5	13.690626967898053	37.73814662034515	33.68730144129738	14.883924970459416
6	32.93264247238219	36.717518957283936	17.34602977834288	13.003808791990995
7	29.2625942294167	30.925716016205975	21.43336262635268	18.378327128024647
8	27.613403994877274	33.94853216916317	19.450297528496932	18.98776630746262
9	27.694733013796142	14.048740035689672	18.597192091666702	39.65933485884749
10	15.64503301511648	27.326483132997286	32.11049096718867	24.917992884697572
11	36.490154318585446	21.15225958919133	23.537865011922364	18.819721080300855
12	24.938212379599932	23.70429962591337	29.71763428945828	21.63985370502841
13	29.835569584158527	20.196235343730255	25.412455786197295	24.55573928591392
14	23.330724796010493	20.193616678034797	25.91961554027855	30.556042985676157
15	24.674703466975743	28.24979886771545	22.694797590516604	24.3807000747922
16	24.81822684497276	26.018891954998942	25.08158211278568	24.081299087242623
17	23.334583353970466	26.353029413221446	25.984886542938206	24.32750068986988
18	23.869794559560745	25.439660936598962	27.553571239046065	23.13697326479422
19	25.423598094967204	24.797974116063788	25.98193317950993	23.796494609459078
20	25.659344313796524	24.773402353926773	25.765054503721085	23.80219882855562
21	26.514635854837227	24.25334852226334	24.91059993914994	24.321415683749496
22	25.5242310947083	25.081227871065614	25.365313151839512	24.02922788238657
23	24.03134233734476	24.920681867068186	26.125454257282264	24.922521538304785
24	24.703530743649615	25.151984716620674	26.152196985777966	23.992287553951744
25	24.569637405052767	24.892431462868423	26.240354917797898	24.297576214280912
26	23.918241250015036	25.86215558532186	26.571947544315115	23.647655620347983
27	24.382919375302055	25.45818895336701	25.837887331617637	24.321004339713294
28	23.917761246940564	25.47219142247202	26.04003110558563	24.570016225001787
29	24.246980598990856	24.936300527072895	25.86371956806769	24.95299930586856
30	23.989357938383595	25.397237592516593	26.23197430055333	24.38143016854648
31	24.937517513494235	24.99716953628513	25.428815252802867	24.636497697417767
32	24.15926830321037	25.652125612854793	25.45414406235782	24.734462021577016
33	23.93318667384633	25.287015540763814	26.118078105021684	24.661719680368172
34	24.42272340678453	25.386089321674355	26.288320594590996	23.90286667695012
35	24.854132614433194	25.42833287340706	25.945219243315147	23.772315268844594
36	24.103124020404596	25.756521849886532	25.81086617638838	24.329487953320488
37	24.765508028201303	25.566771482832696	25.351737957698045	24.315982531267956
38	24.28190322534992	25.73543356118824	25.587540157658612	24.395123055803225
39	24.86672600962035	25.103413823700482	25.37576401694246	24.654096149736706
40	25.37497930343499	25.389343073337304	25.653126985631985	23.582550637595716
41	24.495635634955676	25.454833633348052	26.255037982995393	23.79449274870088
42	25.20296670313015	26.228095374892273	25.437031145854412	23.131906776123163
43	24.182690674848015	25.484116561569554	25.874126863728474	24.459065899853957
44	23.90548328551364	25.87074885285804	25.509677738900937	24.714090122727388
45	24.136062084658843	26.041678459431004	25.398925631997827	24.42333382391233
46	24.21331337061249	25.565042407809802	25.37852870690674	24.84311551467097
47	24.7567549973926	25.43984342882939	25.536143755656145	24.26725781812187
48	24.8499771808234	25.826425667858928	24.8017915707023	24.521805580615375
49	24.457529630942553	25.85991821994293	25.14443116585757	24.538120983256952
50	24.445582847350074	26.430531399872354	24.666911014472653	24.456974738304915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	33.0
2	33.0
3	85.0
4	137.0
5	171.0
6	205.0
7	212.0
8	219.0
9	315.0
10	411.0
11	636.5
12	862.0
13	1517.0
14	2172.0
15	3134.0
16	4096.0
17	4561.0
18	5026.0
19	5103.5
20	5181.0
21	5478.0
22	5775.0
23	6497.0
24	7219.0
25	8086.0
26	8953.0
27	11723.5
28	14494.0
29	17923.5
30	21353.0
31	25092.0
32	28831.0
33	34353.5
34	39876.0
35	46788.0
36	53700.0
37	59703.0
38	65706.0
39	67721.5
40	69737.0
41	73449.5
42	77162.0
43	78010.5
44	78859.0
45	85114.5
46	91370.0
47	95599.5
48	99829.0
49	102116.5
50	104404.0
51	101939.0
52	99474.0
53	97925.0
54	96376.0
55	95337.0
56	94298.0
57	91273.5
58	88249.0
59	79425.5
60	70602.0
61	62394.5
62	54187.0
63	46871.0
64	39555.0
65	33545.5
66	27536.0
67	23656.0
68	19776.0
69	17166.5
70	14557.0
71	11510.0
72	8463.0
73	6995.0
74	5527.0
75	4371.0
76	3215.0
77	2789.5
78	2364.0
79	1916.0
80	1468.0
81	1183.5
82	899.0
83	735.0
84	571.0
85	414.5
86	258.0
87	176.0
88	94.0
89	77.0
90	60.0
91	56.0
92	52.0
93	45.0
94	38.0
95	30.5
96	23.0
97	19.0
98	15.0
99	12.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009835068031783543
2	2.830235404829797E-4
3	6.368029660867042E-4
4	0.0
5	0.0
6	7.075588512074492E-5
7	2.830235404829797E-4
8	0.0
9	0.0011320941619319188
10	0.0012736059321734085
11	0.0
12	7.075588512074492E-5
13	4.952911958452144E-4
14	1.4151177024148985E-4
15	0.00431610899236544
16	7.075588512074492E-4
17	0.0
18	3.537794256037246E-4
19	7.075588512074492E-5
20	8.490706214489391E-4
21	0.0
22	1.4151177024148985E-4
23	9.905823916904288E-4
24	7.075588512074492E-4
25	0.001768897128018623
26	0.005589714924538848
27	0.005872738465021828
28	0.004174597222123951
29	0.0023349442089845825
30	0.003537794256037246
31	0.008066170903764922
32	0.0033255266006750116
33	0.004174597222123951
34	0.002547211864346817
35	0.002830235404829797
36	0.0070048326269537475
37	0.0029717471750712867
38	0.00919826506569684
39	0.003962329566761715
40	0.0022641883238638375
41	0.0032547707155542666
42	0.0016981412428978783
43	9.905823916904288E-4
44	0.0010613382768111738
45	9.905823916904288E-4
46	6.368029660867042E-4
47	0.0016273853577771333
48	0.001768897128018623
49	4.952911958452144E-4
50	0.0016981412428978783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1413310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.279948332166505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75316159703308	38.762846908916835
2	15.143204210418432	16.136582758290807
3	5.385639373663665	8.60839762693445
4	2.484760226043907	5.295515858457669
5	1.304773993410182	3.4759145477024536
6	0.7475744141400456	2.389843569589877
7	0.4661964923741234	1.7387247518431386
8	0.3355279434928684	1.4301529194638491
9	0.2265085341542026	1.086152669687762
>10	1.01553891601188	9.381502150779209
>50	0.06710873521003181	2.5190858693091442
>100	0.06359289404137364	6.693641044824061
>500	0.005744683547185204	2.0793979341978663
>1k	6.679864589750236E-4	0.4022413900028118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.075588512074492E-5
2	0.0	0.0	0.0	0.0	7.075588512074492E-5
3	0.0	0.0	0.0	7.075588512074492E-5	7.075588512074492E-5
4	0.0	0.0	0.0	7.075588512074492E-5	7.075588512074492E-5
5	0.0	0.0	0.0	7.075588512074492E-5	7.075588512074492E-5
6	0.0	0.0	0.0	7.075588512074492E-5	1.4151177024148985E-4
7	0.0	0.0	0.0	7.075588512074492E-5	1.4151177024148985E-4
8	0.0	0.0	0.0	7.075588512074492E-5	1.4151177024148985E-4
9	0.0	0.0	0.0	7.075588512074492E-5	1.4151177024148985E-4
10	0.0	0.0	0.0	1.4151177024148985E-4	2.1226765536223476E-4
11	0.0	0.0	0.0	1.4151177024148985E-4	2.1226765536223476E-4
12	0.0	0.0	0.0	1.4151177024148985E-4	3.537794256037246E-4
13	0.0	0.0	0.0	1.4151177024148985E-4	3.537794256037246E-4
14	0.0	0.0	0.0	1.4151177024148985E-4	3.537794256037246E-4
15	0.0	0.0	0.0	2.1226765536223476E-4	4.245353107244695E-4
16	0.0	0.0	0.0	2.1226765536223476E-4	5.660470809659594E-4
17	0.0	0.0	0.0	2.1226765536223476E-4	5.660470809659594E-4
18	0.0	0.0	0.0	2.1226765536223476E-4	6.368029660867042E-4
19	0.0	0.0	0.0	3.537794256037246E-4	7.075588512074492E-4
20	0.0	0.0	0.0	4.245353107244695E-4	7.783147363281941E-4
21	0.0	0.0	0.0	5.660470809659594E-4	7.783147363281941E-4
22	0.0	0.0	0.0	9.905823916904288E-4	8.49070621448939E-4
23	0.0	0.0	0.0	0.0019104088982601127	8.49070621448939E-4
24	0.0	0.0	0.0	0.003184014830433521	9.19826506569684E-4
25	0.0	0.0	0.0	0.003679306026278736	9.19826506569684E-4
26	0.0	0.0	0.0	0.004386864877486185	9.905823916904288E-4
27	0.0	0.0	0.0	0.004811400188210654	9.905823916904288E-4
28	0.0	0.0	0.0	0.005872738465021828	9.905823916904288E-4
29	0.0	0.0	0.0	0.00792465913352343	9.905823916904288E-4
30	7.075588512074492E-5	0.0	0.0	0.01308983874733781	9.905823916904288E-4
31	7.075588512074492E-5	0.0	0.0	0.02660421280540009	9.905823916904288E-4
32	7.075588512074492E-5	0.0	0.0	0.040613878059307586	9.905823916904288E-4
33	7.075588512074492E-5	0.0	0.0	0.053279181495920924	0.0010613382768111738
34	7.075588512074492E-5	0.0	0.0	0.06566146139205128	0.0011320941619319188
35	7.075588512074492E-5	0.0	0.0	0.08108624434837368	0.0011320941619319188
36	7.075588512074492E-5	0.0	0.0	0.10330359227628758	0.0011320941619319188
37	7.075588512074492E-5	0.0	0.0	0.1357805435467095	0.0011320941619319188
38	1.4151177024148985E-4	0.0	0.0	0.1803567511727788	0.0011320941619319188
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGT	55	0.0044847955	19.999372	44
TAGGACG	845	0.0	19.787062	4
GTATCAA	3930	0.0	19.595034	1
ATATCCG	70	8.125964E-4	18.856552	19
GTCCTAC	1195	0.0	18.780312	1
CCTAGTA	95	1.5978534E-5	18.525734	2
GATATAC	495	0.0	18.22423	1
TAACGGC	145	6.3064363E-9	18.207613	36
GTAGGAC	1105	0.0	18.11772	3
CTGTAGG	1155	0.0	18.097233	1
AGGACGT	920	0.0	17.934853	5
TAGGACC	1025	0.0	17.60007	4
CATACGG	75	0.0012912884	17.599447	16
AACGCAG	4380	0.0	17.52975	6
TCCTACA	1350	0.0	17.43649	2
GGACGTG	930	0.0	17.268887	6
TGTAGGA	1200	0.0	17.049465	2
ACCGTCG	340	0.0	16.823597	8
ATACCGT	385	0.0	16.571495	6
TTAGGAC	605	0.0	16.363703	3
>>END_MODULE