FastQCFastQC Report
Thu 2 Feb 2017
SRR4063902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808575
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27720.15326983951453493No Hit
TATCAACGCAGAGTACTTTTTTTTT18370.10157167936082273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTT400.00528086914.249466
GGTATCA6250.013.6779681
GTATCAA18100.013.381991
CTATTAG655.4610537E-513.1518931
CGAACGA751.4773708E-512.6679416
ACCGTAT550.003067741212.09145458
TTAGACC550.003072830812.08877854
CCTACAC1850.011.8084033
GTGTACG700.001493454710.8555311
GTCCTAC6300.010.7047591
CCGATAA909.511768E-510.5560329
AATACTA909.533137E-510.5536965
CTAAGAC2100.010.4026413
TTAAGGT1106.0585426E-610.361814
ATTACAC2200.010.3615243
GTAGGAC6800.010.3361283
GTTCTAA1201.5266487E-610.2901371
TAGGACA3150.010.2521614
GGACCGG1409.6933945E-810.1781866
TAGGACG4500.010.1315474
Produced by FastQC (version 0.11.5)