##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063902_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1808575
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264733284491935	32.0	32.0	32.0	32.0	32.0
2	30.840487123840592	32.0	32.0	32.0	32.0	32.0
3	30.85987255159449	32.0	32.0	32.0	32.0	32.0
4	30.904109589041095	32.0	32.0	32.0	32.0	32.0
5	30.784420330923517	32.0	32.0	32.0	32.0	32.0
6	34.445515944873726	36.0	36.0	36.0	32.0	36.0
7	34.3912461468283	36.0	36.0	36.0	32.0	36.0
8	34.35781374839307	36.0	36.0	36.0	32.0	36.0
9	34.51379843246755	36.0	36.0	36.0	32.0	36.0
10	34.203588460528316	36.0	36.0	36.0	32.0	36.0
11	34.47461067414954	36.0	36.0	36.0	32.0	36.0
12	34.30139806201014	36.0	36.0	36.0	32.0	36.0
13	34.37640849840344	36.0	36.0	36.0	32.0	36.0
14	34.290256693805894	36.0	36.0	36.0	32.0	36.0
15	34.22020541033687	36.0	36.0	36.0	32.0	36.0
16	34.21365826686756	36.0	36.0	36.0	32.0	36.0
17	34.16112187772141	36.0	36.0	36.0	32.0	36.0
18	34.151132244999516	36.0	36.0	36.0	32.0	36.0
19	34.16302724520686	36.0	36.0	36.0	32.0	36.0
20	34.14366061678393	36.0	36.0	36.0	32.0	36.0
21	34.13241364057338	36.0	36.0	36.0	32.0	36.0
22	34.11131526201567	36.0	36.0	36.0	32.0	36.0
23	34.05563440830488	36.0	36.0	36.0	32.0	36.0
24	34.031921816900045	36.0	36.0	36.0	32.0	36.0
25	33.63217671371107	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	11.0
5	15.0
6	89.0
7	29.0
8	122.0
9	113.0
10	143.0
11	49.0
12	102.0
13	110.0
14	140.0
15	252.0
16	479.0
17	690.0
18	838.0
19	1153.0
20	1725.0
21	2483.0
22	3882.0
23	6011.0
24	8906.0
25	13442.0
26	19324.0
27	26186.0
28	35901.0
29	48986.0
30	65259.0
31	89135.0
32	125879.0
33	178165.0
34	376228.0
35	802724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95806137575502	17.78525123348895	12.437883045334868	25.818804345421153
2	16.101340184010578	19.70480231221416	38.367626347607356	25.826231156167907
3	18.719008355855955	23.808604894965697	28.75427413754208	28.718112611636272
4	12.862242511304785	16.218290475226787	36.10061453360361	34.81885247986482
5	14.61739145821675	36.46907701053069	34.067488448530995	14.846043082721568
6	33.5128911687075	35.63197224401948	17.391616910842096	13.463519676430918
7	29.92730723753123	30.467580819817737	21.005354620558602	18.59975732209243
8	27.65810574012779	33.648191496893645	19.412369240844647	19.28133352213392
9	27.04778964127205	14.965815545006814	18.784771529236437	39.2016232844847
10	15.835832125430144	27.152372838222117	32.12836230456864	24.883432731779102
11	35.85880491488573	21.526026654569392	23.312458162062878	19.302710268481995
12	24.578925062437825	23.547552890951522	29.433694183572186	22.439827863038467
13	29.415276410713503	20.251565645919197	25.145332994725578	25.187824948641723
14	23.32698153441314	19.78186992976504	25.691145437383323	31.2000030984385
15	24.824520394472952	27.71319105381817	22.340004702692983	25.122283849015894
16	25.1521135441778	26.06996808063885	24.355374420358455	24.422543954824892
17	23.650271821218762	26.228174908350937	25.608513265738576	24.513040004691725
18	24.12401517071481	25.704611529876058	26.399622392187545	23.771750907221584
19	25.341727086453925	25.22270430169703	25.44359642477909	23.991972187069955
20	25.601411746737767	24.952645724723773	25.41658758147619	24.029354947062266
21	25.862765408817562	24.684154377018302	24.898241047536203	24.554839166627936
22	25.48790994866494	24.873018077666067	25.20298570066519	24.436086273003802
23	24.466897095949115	25.117566525110924	25.436647995475532	24.97888838346443
24	24.62129701104775	25.114447847583634	25.599907036197784	24.664348105170834
25	24.689909718975063	24.88983944198967	25.663494979622442	24.756755859412827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	70.0
2	210.5
3	351.0
4	351.0
5	351.0
6	871.0
7	1391.0
8	1391.0
9	1391.0
10	1793.0
11	2195.0
12	2195.0
13	2195.0
14	3405.5
15	4616.0
16	4616.0
17	4616.0
18	8593.0
19	12570.0
20	12570.0
21	12570.0
22	19776.0
23	26982.0
24	26982.0
25	26982.0
26	40892.0
27	54802.0
28	54802.0
29	54802.0
30	70679.0
31	86556.0
32	86556.0
33	86556.0
34	104207.0
35	121858.0
36	121858.0
37	121858.0
38	141521.5
39	161185.0
40	161185.0
41	161185.0
42	180385.0
43	199585.0
44	199585.0
45	199585.0
46	217030.0
47	234475.0
48	234475.0
49	234475.0
50	240510.5
51	246546.0
52	246546.0
53	246546.0
54	229942.5
55	213339.0
56	213339.0
57	213339.0
58	193135.5
59	172932.0
60	172932.0
61	172932.0
62	147913.5
63	122895.0
64	122895.0
65	122895.0
66	99242.5
67	75590.0
68	75590.0
69	75590.0
70	56764.0
71	37938.0
72	37938.0
73	37938.0
74	27956.5
75	17975.0
76	17975.0
77	17975.0
78	13202.0
79	8429.0
80	8429.0
81	8429.0
82	5807.0
83	3185.0
84	3185.0
85	3185.0
86	2328.5
87	1472.0
88	1472.0
89	1472.0
90	1023.0
91	574.0
92	574.0
93	574.0
94	413.0
95	252.0
96	252.0
97	252.0
98	532.0
99	812.0
100	812.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03842804417842777
2	0.00508687779052569
3	0.0012717194476314225
4	0.0027093153449539
5	0.0081279460348617
6	0.013491284574872482
7	0.02305682650705666
8	0.03710103258089932
9	0.0487676762091702
10	0.057227375143414014
11	0.05556861064650346
12	0.06507886042879062
13	0.06491298397909957
14	0.06706937782508328
15	0.06082136488672021
16	0.06679291707559819
17	0.06286717443290989
18	0.07790663920489889
19	0.07508673956015094
20	0.08028420165047068
21	0.07536320030963603
22	0.07895719005294224
23	0.0836570227941888
24	0.07851485285376608
25	0.07962069585170646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1808575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.513994100093335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.6328099201689	31.65990937419546
2	16.425713126077092	15.60902473124505
3	6.779091684014996	9.663051668348409
4	3.403640224138239	6.468821661141785
5	1.9250294309564497	4.5732918512485385
6	1.2137121310454382	3.4600986620222796
7	0.7918453560210897	2.6336614901920656
8	0.5420238667512141	2.0602975045541574
9	0.3817767785710627	1.63257656441203
>10	1.7723336120658881	14.289259080555228
>50	0.08480769417925026	2.7769088820244576
>100	0.04394418656275217	3.9631899315693144
>500	0.002921419149667577	0.8980341274836996
>1k	3.505702979601093E-4	0.3118744710075393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2772	0.15326983951453493	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1837	0.10157167936082273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.5292149897018373E-5	0.0
5	0.0	0.0	0.0	5.5292149897018373E-5	0.0
6	0.0	0.0	0.0	5.5292149897018373E-5	0.0
7	0.0	0.0	0.0	5.5292149897018373E-5	0.0
8	0.0	0.0	0.0	5.5292149897018373E-5	0.0
9	0.0	0.0	0.0	5.5292149897018373E-5	0.0
10	0.0	0.0	0.0	5.5292149897018373E-5	0.0
11	0.0	0.0	0.0	5.5292149897018373E-5	0.0
12	0.0	0.0	0.0	5.5292149897018373E-5	0.0
13	0.0	0.0	0.0	5.5292149897018373E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTT	40	0.005280869	14.24946	6
GGTATCA	625	0.0	13.677968	1
GTATCAA	1810	0.0	13.38199	1
CTATTAG	65	5.4610537E-5	13.151893	1
CGAACGA	75	1.4773708E-5	12.66794	16
ACCGTAT	55	0.0030677412	12.0914545	8
TTAGACC	55	0.0030728308	12.0887785	4
CCTACAC	185	0.0	11.808403	3
GTGTACG	70	0.0014934547	10.855531	1
GTCCTAC	630	0.0	10.704759	1
CCGATAA	90	9.511768E-5	10.556032	9
AATACTA	90	9.533137E-5	10.553696	5
CTAAGAC	210	0.0	10.402641	3
TTAAGGT	110	6.0585426E-6	10.36181	4
ATTACAC	220	0.0	10.361524	3
GTAGGAC	680	0.0	10.336128	3
GTTCTAA	120	1.5266487E-6	10.290137	1
TAGGACA	315	0.0	10.252161	4
GGACCGG	140	9.6933945E-8	10.178186	6
TAGGACG	450	0.0	10.131547	4
>>END_MODULE