##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1808575
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32264075307908	32.0	32.0	32.0	32.0	32.0
2	31.343610300927526	32.0	32.0	32.0	32.0	32.0
3	31.43939897433062	32.0	32.0	32.0	32.0	32.0
4	31.533877776702653	32.0	32.0	32.0	32.0	32.0
5	31.468314004119264	32.0	32.0	32.0	32.0	32.0
6	35.05157762326694	36.0	36.0	36.0	36.0	36.0
7	35.07910869054366	36.0	36.0	36.0	36.0	36.0
8	35.013320431831694	36.0	36.0	36.0	36.0	36.0
9	35.14726345327122	36.0	36.0	36.0	36.0	36.0
10	34.977912997802136	36.0	36.0	36.0	36.0	36.0
11	35.13662800823853	36.0	36.0	36.0	36.0	36.0
12	35.045318551898596	36.0	36.0	36.0	36.0	36.0
13	35.0932076358459	36.0	36.0	36.0	36.0	36.0
14	35.035678365564046	36.0	36.0	36.0	36.0	36.0
15	34.996535946808955	36.0	36.0	36.0	36.0	36.0
16	35.02817522082302	36.0	36.0	36.0	36.0	36.0
17	34.991324108759656	36.0	36.0	36.0	36.0	36.0
18	34.9917824806823	36.0	36.0	36.0	36.0	36.0
19	34.98509821268125	36.0	36.0	36.0	36.0	36.0
20	34.977023900031796	36.0	36.0	36.0	36.0	36.0
21	34.97861023181234	36.0	36.0	36.0	36.0	36.0
22	34.95894060240797	36.0	36.0	36.0	36.0	36.0
23	34.91025807610964	36.0	36.0	36.0	32.0	36.0
24	34.886430477033024	36.0	36.0	36.0	32.0	36.0
25	34.86155840924485	36.0	36.0	36.0	32.0	36.0
26	34.80996972754793	36.0	36.0	36.0	32.0	36.0
27	34.79493745075543	36.0	36.0	36.0	32.0	36.0
28	34.77816347124117	36.0	36.0	36.0	32.0	36.0
29	34.75418326583083	36.0	36.0	36.0	32.0	36.0
30	34.737670265264086	36.0	36.0	36.0	32.0	36.0
31	34.72999737362288	36.0	36.0	36.0	32.0	36.0
32	34.68873063046874	36.0	36.0	36.0	32.0	36.0
33	34.677898898303916	36.0	36.0	36.0	32.0	36.0
34	34.67403010657562	36.0	36.0	36.0	32.0	36.0
35	34.64404296200047	36.0	36.0	36.0	32.0	36.0
36	34.62246354173866	36.0	36.0	36.0	32.0	36.0
37	34.59880071326874	36.0	36.0	36.0	32.0	36.0
38	34.57391039907109	36.0	36.0	36.0	32.0	36.0
39	34.56097424768119	36.0	36.0	36.0	32.0	36.0
40	34.55724810969963	36.0	36.0	36.0	32.0	36.0
41	34.54004506310216	36.0	36.0	36.0	32.0	36.0
42	34.497791907993864	36.0	36.0	36.0	32.0	36.0
43	34.48251966327081	36.0	36.0	36.0	32.0	36.0
44	34.43548594888241	36.0	36.0	36.0	32.0	36.0
45	34.41075929944846	36.0	36.0	36.0	32.0	36.0
46	34.389880983647345	36.0	36.0	36.0	32.0	36.0
47	34.36615733381253	36.0	36.0	36.0	32.0	36.0
48	34.333870588723165	36.0	36.0	36.0	32.0	36.0
49	34.315841477406245	36.0	36.0	36.0	32.0	36.0
50	33.71964613024066	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	6.0
20	16.0
21	53.0
22	173.0
23	493.0
24	1241.0
25	2940.0
26	6090.0
27	11274.0
28	19187.0
29	30242.0
30	44262.0
31	64794.0
32	95747.0
33	155828.0
34	327619.0
35	1048607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.098013388586416	18.73301681149034	12.982704105162227	26.186265694761023
2	15.371830088777418	20.494944066244674	37.776185357697365	26.357040487280543
3	17.95649714608937	24.430061081373083	29.0951253779918	28.51831639454575
4	12.514161701892373	16.998907980039533	36.1557579862599	34.33117233180819
5	14.276709564159628	37.00172787968428	33.732634809172964	14.988927746983121
6	33.602348370787354	36.03349159807207	17.062512820881434	13.301647210259137
7	29.9378792695907	30.585903266383756	21.009081735620587	18.46713572840496
8	27.656469872689826	33.86577830612499	19.253832437139735	19.223919384045452
9	27.03963232403435	14.856771920473264	18.63698467666621	39.466611078826176
10	15.6244747202194	27.39975450081833	32.13606405095767	24.8397067280046
11	36.08476286579213	21.530376124849674	23.10891171225965	19.275949297098542
12	24.570655743868645	23.610063630311647	29.381467809962775	22.43781281585693
13	29.574353618085482	20.17735588452073	25.14327641718554	25.105014080208253
14	23.344478424655073	19.731732657144956	25.62711182155616	31.296677096643812
15	24.87499357204589	27.81482183922982	22.22938589676457	25.08079869195972
16	25.250572139949938	26.122536093612545	24.249831772331703	24.377059994105814
17	23.69202272507361	26.293628962028116	25.530873754198748	24.483474558699527
18	24.239579203691754	25.761775456976803	26.309721874341673	23.688923464989763
19	25.434831289827624	25.247003856627455	25.395795032000336	23.922369821544585
20	25.820401356654944	24.938763639258948	25.259238534839206	23.981596469246906
21	25.96701823258643	24.76939026581701	24.796151666367166	24.467439835229392
22	25.57140416383985	24.95041665491706	25.164674209638438	24.313504971604655
23	24.679427101908207	25.136144300517703	25.293451858634413	24.890976738939674
24	24.744093258261092	25.170452440575946	25.501929978894918	24.583524322268048
25	24.920170722685118	24.837618264828063	25.565716566945728	24.67649444554109
26	24.379509977937396	25.48529436159448	25.73041598239415	24.40477967807397
27	24.759504394087102	25.51440374729119	25.161892512550782	24.56419934607092
28	24.635209745065676	25.105045327494256	25.391912104196564	24.867832823243507
29	24.655950430542322	25.180850999124704	25.225859854368153	24.93733871596482
30	24.598274936370377	25.156578913173423	25.52140902498351	24.723737125472695
31	25.147655293112553	24.847285083511434	25.100598030894115	24.904461592481898
32	24.698578334797705	25.339049263123904	25.034655542979234	24.92771685909916
33	24.515037406897466	25.31703244142904	25.309899418852194	24.858030732821305
34	24.879141173835993	25.107505328957735	25.650768724616608	24.362584772589667
35	24.935983531276143	25.17380243171346	25.518559661247686	24.37165437576271
36	24.54168986810157	25.259879111077687	25.5378539589712	24.660577061849544
37	25.18632752173199	24.987489694549488	25.007008535760672	24.819174247957847
38	24.74629787328165	25.320832549960738	25.163181119430224	24.76968845732739
39	25.154189065598224	25.159220876596773	24.885567770752903	24.8010222870521
40	25.2900841402976	25.134598488161913	25.25757149326496	24.317745878275524
41	24.82891899364114	25.039590821122477	25.572131600774124	24.559358584462263
42	25.09332149316601	25.35955937768346	25.230117448783073	24.317001680367458
43	24.769940134394883	25.00139614531191	25.396187334501484	24.83247638579172
44	24.452920201022142	25.309072370956514	25.276947312138905	24.961060115882443
45	24.58931278800633	25.408862549860274	25.168836540130958	24.83298812200244
46	24.796881479638383	25.01119681512814	25.12432611760803	25.067595587625448
47	25.000649696412076	25.024425820428338	25.34910814652949	24.625816336630105
48	24.819993121531045	25.469577151097404	24.93649580711977	24.773933920251785
49	24.858424058786778	25.183542799006954	25.08611776154642	24.87191538065986
50	24.595823737735376	25.63952990094302	25.030037792602382	24.734608568719228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	18.0
2	29.0
3	69.5
4	110.0
5	119.0
6	128.0
7	133.0
8	138.0
9	191.5
10	245.0
11	400.0
12	555.0
13	1000.0
14	1445.0
15	1980.0
16	2515.0
17	2547.5
18	2580.0
19	2705.0
20	2830.0
21	3454.0
22	4078.0
23	5350.5
24	6623.0
25	8850.5
26	11078.0
27	14389.0
28	17700.0
29	21605.5
30	25511.0
31	30020.5
32	34530.0
33	40731.0
34	46932.0
35	54444.5
36	61957.0
37	67933.0
38	73909.0
39	79670.5
40	85432.0
41	91764.0
42	98096.0
43	101469.0
44	104842.0
45	112511.5
46	120181.0
47	124452.0
48	128723.0
49	132684.5
50	136646.0
51	137398.5
52	138151.0
53	137896.5
54	137642.0
55	133186.5
56	128731.0
57	122318.0
58	115905.0
59	105895.5
60	95886.0
61	84296.0
62	72706.0
63	62748.5
64	52791.0
65	44197.0
66	35603.0
67	29858.0
68	24113.0
69	20143.0
70	16173.0
71	12487.0
72	8801.0
73	7321.5
74	5842.0
75	4733.0
76	3624.0
77	3023.0
78	2422.0
79	1961.0
80	1500.0
81	1172.5
82	845.0
83	663.0
84	481.0
85	364.0
86	247.0
87	175.5
88	104.0
89	80.0
90	56.0
91	45.0
92	34.0
93	35.0
94	36.0
95	30.5
96	25.0
97	25.0
98	25.0
99	18.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009344373332596105
2	3.8704504927912866E-4
3	2.2116859958807347E-4
4	0.0
5	0.0
6	1.1058429979403673E-4
7	0.0
8	0.0
9	0.0014375958973224777
10	8.293822484552755E-4
11	0.0
12	1.6587644969105511E-4
13	4.4233719917614693E-4
14	3.3175289938211023E-4
15	0.003759866192997249
16	7.740900985582573E-4
17	0.0
18	2.2116859958807347E-4
19	0.0
20	4.976293490731653E-4
21	0.0
22	2.2116859958807347E-4
23	8.846743983522939E-4
24	3.3175289938211023E-4
25	0.0015481801971165146
26	0.004699832741246562
27	0.006745642287436241
28	0.0038704504927912857
29	0.0023222702956747715
30	0.003428113293615139
31	0.0077409009855825715
32	0.003428113293615139
33	0.004699832741246562
34	0.003317528993821102
35	0.0030963603942330293
36	0.007630316685788535
37	0.0036492818932032127
38	0.008570283234037848
39	0.004478664141658488
40	0.002654023195056882
41	0.004146911242276377
42	0.0023222702956747715
43	0.001327011597528441
44	9.952586981463306E-4
45	0.0011611351478373858
46	0.0013823037474254593
47	0.002101101696086698
48	0.0018246409466016063
49	2.7646074948509185E-4
50	0.0011058429979403674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1808575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.676174111878694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.27462896141608	33.41946181599342
2	16.293405856527173	16.187881324086955
3	6.70794133619193	9.996745852468157
4	3.303232307725553	6.563677730022296
5	1.8806504764138439	4.671176025496085
6	1.154205256431349	3.4401900767597566
7	0.735214486939385	2.5565850003943513
8	0.5335916111148188	2.120543182270208
9	0.38887856307755025	1.7386199287035826
>10	1.6246461453898347	13.532255761498678
>50	0.06822375887953441	2.3000482744499355
>100	0.0339213716066781	3.0406476128126863
>500	0.001459868286294481	0.4321674150437631
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.5292149897018373E-5	0.0
5	0.0	0.0	0.0	5.5292149897018373E-5	0.0
6	0.0	0.0	0.0	1.1058429979403675E-4	0.0
7	0.0	0.0	0.0	1.1058429979403675E-4	0.0
8	0.0	0.0	0.0	1.1058429979403675E-4	0.0
9	0.0	0.0	0.0	1.1058429979403675E-4	0.0
10	0.0	0.0	0.0	1.6587644969105511E-4	0.0
11	0.0	0.0	0.0	1.6587644969105511E-4	0.0
12	0.0	0.0	0.0	1.6587644969105511E-4	0.0
13	0.0	0.0	0.0	1.6587644969105511E-4	0.0
14	0.0	0.0	0.0	1.6587644969105511E-4	0.0
15	0.0	0.0	0.0	1.6587644969105511E-4	0.0
16	0.0	0.0	0.0	1.6587644969105511E-4	0.0
17	0.0	0.0	0.0	1.6587644969105511E-4	0.0
18	0.0	0.0	0.0	1.6587644969105511E-4	0.0
19	0.0	0.0	0.0	1.6587644969105511E-4	0.0
20	0.0	0.0	0.0	2.211685995880735E-4	0.0
21	0.0	0.0	0.0	2.211685995880735E-4	0.0
22	0.0	0.0	0.0	2.211685995880735E-4	0.0
23	0.0	0.0	0.0	3.3175289938211023E-4	0.0
24	0.0	0.0	0.0	4.42337199176147E-4	0.0
25	0.0	0.0	0.0	4.976293490731653E-4	0.0
26	0.0	0.0	0.0	8.84674398352294E-4	0.0
27	0.0	0.0	0.0	0.001050550848043349	0.0
28	0.0	0.0	0.0	0.0014375958973224777	0.0
29	0.0	0.0	0.0	0.002985776094438992	0.0
30	0.0	0.0	0.0	0.004865709190937617	0.0
31	0.0	0.0	0.0	0.012938363075902298	0.0
32	0.0	0.0	0.0	0.02200627565901331	0.0
33	0.0	0.0	0.0	0.03179298619078556	0.0
34	0.0	0.0	0.0	0.042962000469983275	0.0
35	0.0	0.0	0.0	0.05534744204691539	0.0
36	0.0	0.0	0.0	0.07381502011251953	0.0
37	0.0	0.0	0.0	0.10289869095835119	0.0
38	0.0	0.0	0.0	0.14381488188214478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	30	0.0057484056	29.330566	5
GTATCAA	2110	0.0	29.194792	1
GGTATCA	850	0.0	23.812155	1
ATTCCGC	60	2.873224E-4	22.00036	23
TATCAAC	3110	0.0	19.522816	2
TATACCG	125	2.2922904E-8	19.358177	5
TCAACGC	3130	0.0	18.97585	4
ATCAACG	3135	0.0	18.945581	3
CAACGCA	3195	0.0	18.520945	5
GTATTAG	230	0.0	18.17421	1
AACGCAG	3265	0.0	18.123865	6
GTCCTAC	645	0.0	17.395634	1
GTAGGAC	855	0.0	16.980856	3
TACCGTA	105	3.7790647E-5	16.760326	7
GCGAATC	120	6.4141313E-6	16.4989	15
TAGGACG	655	0.0	16.456465	4
GTATGCG	125	9.448859E-6	15.839384	11
GTATAAT	295	0.0	15.661273	1
TAACGTC	85	0.0029825785	15.527947	5
TTAGGAC	585	0.0	15.417352	3
>>END_MODULE