FastQCFastQC Report
Thu 2 Feb 2017
SRR4063901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302252
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28370.21785338014454958No Hit
TATCAACGCAGAGTACTTTTTTTTT20690.15887861949914456No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14960.11487791917386189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT802.0009593E-613.0611796
GTCTTAG1102.752131E-912.952241
GTATTAG1550.012.8686771
GCTCGTA1207.385097E-1012.6673339
GTCCTAA1551.8189894E-1212.2558831
CGTCTTA701.0885381E-412.21680615
ACGCGCG550.003063246812.09340413
GGCTAAG550.003072077212.08875751
AAGCTCG1201.0002623E-811.8751687
CGAACGA1459.640644E-1111.79553716
CGTCAAT1459.640644E-1111.7946319
CCGATAA1459.640644E-1111.7937239
CGATAAC1459.640644E-1111.79372310
ATTAACC1459.822543E-1111.7878343
CGAGCCG2100.011.76433315
CGGTCCA2100.011.76252310
GTGCGTA658.0183404E-411.69292310
ATTAGCC658.052013E-411.6870833
TCGCCAG1552.5465852E-1111.64711717
ACGAACG1501.7462298E-1011.40235215
Produced by FastQC (version 0.11.5)